Human IL-5 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-5 Quantikine ELISA Kit Summary

• Background: The Quantikine Human IL-5 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-5 levels in cell culture supernates, serum, plasma, and urine. It contains Sf 21-expressed recombinant human IL-5 and antibodies raised against the recombinant factor and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-5 showed linear c...urves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-5.
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• Specificity: Natural and recombinant human IL-5
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL5

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-5 Quantikine ELISA Kit

• BCDF mu
• B-cell differentiation factor I
• BCGFII
• EDF
• Eo-CSF
• Eosinophil differentiation factor
• IL5
• IL-5
• IL-5T-cell replacing factor
• interleukin 5 (colony-stimulating factor, eosinophil)
• interleukin-5
• TRF
• TRFB cell differentiation factor I

Background

IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-5 (D5000B)(44)

Publications using D5000B

• Dolna-Michno, J;Kopi?ski, P;Przybylski, G;Wypasek, E;Szyma?ska, M;W?drowska, E;Miko?ajczyk, K;Senderek, T;Gagat, M; Cytoimmunological Profile of Lower Airways in Post-COVID-19 Syndrome (PCS): Predictive Value of Bronchoalveolar Lavage Journal of clinical medicine 2025-05-12 [PMID: 40429360] (Human)
• Scovino, AM;Dahab, EC;Diniz-Lima, I;de Senna Silveira, E;Barroso, SPC;Cardoso, KM;Nico, D;Makhoul, GJ;da Silva-Junior, EB;Freire-de-Lima, CG;Freire-de-Lima, L;Fonseca, LMD;Valente, N;Nacife, V;Machado, A;Araújo, M;Vieira, GF;Pauvolid-Corrêa, A;Siqueira, M;Morrot, A; A Comparative Analysis of Innate Immune Responses and the Structural Characterization of Spike from SARS-CoV-2 Gamma Variants and Subvariants Microorganisms 2024-04-02 [PMID: 38674664] (Human)
• Irie, M;Kabata, H;Sasahara, K;Kurihara, M;Shirasaki, Y;Kamatani, T;Baba, R;Matsusaka, M;Koga, S;Masaki, K;Miyata, J;Araki, Y;Kikawada, T;Kabe, Y;Suematsu, M;Yamagishi, M;Uemura, S;Moro, K;Fukunaga, K; Annexin A1 is a cell-intrinsic metalloregulator of zinc in human ILC2s Cell reports 2023-06-07 [PMID: 37294636] (Human)
• MP Crawford, N Borcherdin, NJ Karandikar IL-17 cytokines preferentially act on na�ve CD4+ T cells with the IL-17AF heterodimer inducing the greatest functional changes PLoS ONE, 2023-04-28;18(4):e0285166. 2023-04-28 [PMID: 37115755] (Human)
• MP Crawford, N Borcherdin, NJ Karandikar IL-17 cytokines preferentially act on na�ve CD4+ T cells with the IL-17AF heterodimer inducing the greatest functional changes PLoS ONE, 2023;18(4):e0285166. 2023 [PMID: 37115755] (Human)
• MK Šoki?, M Rijavec, P Korošec, U Bidovec-St, I Kern, R Vantur, S Škrgat Heterogeneous Response of Airway Eosinophilia to Anti-IL-5 Biologics in Severe Asthma Patients Journal of personalized medicine, 2022-01-07;12(1):. 2022-01-07 [PMID: 35055384] (Human)
• Y Jiang, AK Farrell, ET Tobin, HE Mair-Meije, DE Wildman, F Luca, RB Slatcher, S Zilioli Socioeconomic status, financial stress, and glucocorticoid resistance among youth with asthma: Testing the moderation effects of maternal involvement and warmth Brain, Behavior, and Immunity, 2021-05-17;0(0):. 2021-05-17 [PMID: 34015429] (Human)
• T Tanosaki, H Kabata, M Matsusaka, J Miyata, K Masaki, T Mochimaru, S Okuzumi, M Kuwae, R Watanabe, Y Suzuki, K Sayama, K Izuhara, K Asano, K Fukunaga Clinical characteristics of patients with not well-controlled severe asthma in Japan: Analysis of the Keio Severe Asthma Research Program in Japanese population (KEIO-SARP) registry Allergol Int, 2020-07-08;0(0):. 2020-07-08 [PMID: 32651123] (Human)
• S Firouzmand, L Ajori, JS Young New participant stratification and combination of urinary biomarkers and confounders could improve diagnostic accuracy for overactive bladder Sci Rep, 2020-02-20;10(1):3085. 2020-02-20 [PMID: 32080289] (Human)
• M Al-Ayed, K Alshaybari, D Alshehri, A Jamaan, I Nasser, H Alaamri, W Alaseeri, AA Mahfouz, S Ali Alsare, AM Asaad, A Ali Magzou, MA Qureshi, MH Shalayel Obesity and childhood asthma in male schoolchildren in Saudi Arabia: Is there a role for leptin, interleukin-4, interleukin-5, and interleukin-21? Ann Saudi Med, 2019-10-03;39(5):295-301. 2019-10-03 [PMID: 31580718] (Human)
• M Watanabe, K Nakamoto, T Inui, M Sada, K Honda, M Tamura, Y Ogawa, T Yokoyama, T Saraya, D Kurai, H Ishii, H Takizawa Serum sST2 levels predict severe exacerbation of asthma Respir. Res., 2018-09-03;19(1):169. 2018-09-03 [PMID: 30176857] (Human)
• Schiavoni I, Fedele G, Quattrini A, Bianco M, Schnoeller C, Openshaw P, Locht C, Ausiello C Live attenuated B. pertussis BPZE1 rescues the immune functions of Respiratory Syncytial virus infected human dendritic cells by promoting Th1/Th17 responses. PLoS ONE, 2014-06-26;9(6):e100166. 2014-06-26 [PMID: 24967823] (Human)
• Arndts , Kathrin, Specht , Sabine, Debrah , Alexande, Tamarozzi , Francesc, Klarmann Schulz , Ute, Mand , Sabine, Batsa , Linda, Kwarteng , Alexande, Taylor , Mark, Adjei , Ohene, Martin , Coralie, Layland , Laura E, Hoerauf , Achim Immunoepidemiological profiling of onchocerciasis patients reveals associations with microfilaria loads and ivermectin intake on both individual and community levels. PLoS Negl Trop Dis, 2014-02-20;8(2):e2679. 2014-02-20 [PMID: 24587458] (Human)
• Biancheri P, Di Sabatino A, Ammoscato F, Facciotti F, Caprioli F, Curciarello R, Hoque S, Ghanbari A, Joe-Njoku I, Giuffrida P, Rovedatti L, Geginat J, Corazza G, MacDonald T Absence of a role for interleukin-13 in inflammatory bowel disease. Eur J Immunol, 2014-02-01;44(2):370-85. 2014-02-01 [PMID: 24338958] (Human)
• Ishigami , Tomoaki, Abe , Kaito, Aoki , Ichiro, Minegishi , Shintaro, Ryo , Akihide, Matsunaga , Satoko, Matsuoka , Kazuhiro, Takeda , Hiroyuki, Sawasaki , Tatsuya, Umemura , Satoshi, Endo , Yaeta Anti-interleukin-5 and multiple autoantibodies are associated with human atherosclerotic diseases and serum interleukin-5 levels. FASEB J, 2013-05-22;27(9):3437-45. 2013-05-22 [PMID: 23699176] (Human)
• Bachert C, Zhang N, Holtappels G, De Lobel L, Van Cauwenberge P, Liu S, Lin P, Bousquet J, Van Steen K Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma. J. Allergy Clin. Immunol., 2010-11-01;126(5):962. 2010-11-01 [PMID: 20810157] (Human)
• Niederfuhr A, Kirsche H, Deutschle T, Poppert S, Riechelmann H, Wellinghausen N Staphylococcus aureus in nasal lavage and biopsy of patients with chronic rhinosinusitis. Allergy, 2008-10-01;63(10):1359-67. 2008-10-01 [PMID: 18782116] (Human)
• Hothersall EJ, Chaudhuri R, McSharry C, Donnelly I, Lafferty J, McMahon AD, Weir CJ, Meiklejohn J, Sattar N, McInnes I, Wood S, Thomson NC Effects of atorvastatin added to inhaled corticosteroids on lung function and sputum cell counts in atopic asthma. Thorax, 2008-08-29;63(12):1070-5. 2008-08-29 [PMID: 18757458] (Human)
• Yokoi T, Amakawa R, Tanijiri T, Sugimoto H, Torii Y, Amuro H, Son Y, Tajima K, Liu YJ, Ito T, Fukuhara S Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells. Int. Immunol., 2008-08-13;20(10):1321-9. 2008-08-13 [PMID: 18703465] (Human)
• Ingvarsson J, Wingren C, Carlsson A, Ellmark P, Wahren B, Engstrom G, Harmenberg U, Krogh M, Peterson C, Borrebaeck CA Detection of pancreatic cancer using antibody microarray-based serum protein profiling. Proteomics, 2008-06-01;8(11):2211-9. 2008-06-01 [PMID: 18528842] (Human)
• Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. Eur. J. Immunol., 2008-04-01;38(4):1012-23. 2008-04-01 [PMID: 18350544] (Human)
• Makowska JS, Grzegorczyk J, Bienkiewicz B, Wozniak M, Kowalski ML Systemic responses after bronchial aspirin challenge in sensitive patients with asthma. J. Allergy Clin. Immunol., 2007-12-20;121(2):348-54. 2007-12-20 [PMID: 18086495] (Human)
• Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema. J. Allergy Clin. Immunol., 2007-11-01;120(5):1118-25. 2007-11-01 [PMID: 17983873] (Human)
• Vidyarani M, Selvaraj P, Jawahar MS, Narayanan PR 1, 25 Dihydroxyvitamin D3 modulated cytokine response in pulmonary tuberculosis. Cytokine, 2007-10-29;40(2):128-34. 2007-10-29 [PMID: 17911026] (Human)
• Lin YL, Liang YC, Chiang BL Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells. J. Leukoc. Biol., 2007-08-30;82(6):1473-80. 2007-08-30 [PMID: 17761954] (Human)
• Emad A, Emad Y Relationship between eosinophilia and levels of chemokines (CCL5 and CCL11) and IL-5 in bronchoalveolar lavage fluid of patients with mustard gas-induced pulmonary fibrosis. J. Clin. Immunol., 2007-07-10;27(6):605-12. 2007-07-10 [PMID: 17620002] (Human)
• Kocak AK, Bor O, Yildiz B, Erdogan L, Us T T-lymphocyte activation and the levels of eosinophilic cationic protein and interleukin-5 in asthmatic children with acute exacerbation and effect of glucocorticoid treatment. Allergy Asthma Proc, 2006-07-01;27(4):371-7. 2006-07-01 [PMID: 16948352] (Human)
• Benson M, Langston MA, Adner M, Andersson B, Torinssson-Naluai A, Cardell LO A network-based analysis of the late-phase reaction of the skin. J. Allergy Clin. Immunol., 2006-05-11;118(1):220-5. 2006-05-11 [PMID: 16815159] (Human)
• Hartl D, Latzin P, Zissel G, Krane M, Krauss-Etschmann S, Griese M Chemokines indicate allergic bronchopulmonary aspergillosis in patients with cystic fibrosis. Am. J. Respir. Crit. Care Med., 2006-03-16;173(12):1370-6. 2006-03-16 [PMID: 16543550] (Human)
• Kristjansson S, Bjarnarson SP, Wennergren G, Palsdottir AH, Arnadottir T, Haraldsson A, Jonsdottir I Respiratory syncytial virus and other respiratory viruses during the first 3 months of life promote a local TH2-like response. J. Allergy Clin. Immunol., 2005-10-01;116(4):805-11. 2005-10-01 [PMID: 16210054] (Human)
• Kalomenidis I, Stathopoulos GT, Barnette R, Guo Y, Peebles RS, Blackwell TS, Light RW Eotaxin-3 and interleukin-5 pleural fluid levels are associated with pleural fluid eosinophilia in post-coronary artery bypass grafting pleural effusions. Chest, 2005-06-01;127(6):2094-100. 2005-06-01 [PMID: 15947325] (Human)
• Ciprandi G, Tosca MA, Marseglia GL, Klersy C Relationships between allergic inflammation and nasal airflow in children with seasonal allergic rhinitis. Ann. Allergy Asthma Immunol., 2005-02-01;94(2):258-61. 2005-02-01 [PMID: 15765742] (Human)
• Pitzurra L, Bellocchio S, Nocentini A, Bonifazi P, Scardazza R, Gallucci L, Stracci F, Simoncelli C, Bistoni F, Romani L Antifungal immune reactivity in nasal polyposis. Infect. Immun., 2004-12-01;72(12):7275-81. 2004-12-01 [PMID: 15557653] (Human)
• Saeki S, Matsuse H, Kondo Y, Machida I, Kawano T, Tomari S, Obase Y, Fukushima C, Kohno S Effects of antiasthmatic agents on the functions of peripheral blood monocyte-derived dendritic cells from atopic patients. J. Allergy Clin. Immunol., 2004-09-01;114(3):538-44. 2004-09-01 [PMID: 15356554] (Human)
• Chen LL, Tager IB, Peden DB, Christian DL, Ferrando RE, Welch BS, Balmes JR Effect of ozone exposure on airway responses to inhaled allergen in asthmatic subjects. Chest, 2004-06-01;125(6):2328-35. 2004-06-01 [PMID: 15189958] (Human)
• Hellman C, 107334, Hallden G, Hylander B, Lundahl J Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects. Lagging strand gap suppression connects BRCA-mediated fork protection to nucleosome assembly through PCNA-dependent CAF-1 recycling, 2003-01-01;131(1):75-81. 2003-01-01 [PMID: 12519389] (Human)
• Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta. J. Allergy Clin. Immunol., 2002-10-01;110(4):624-33. 2002-10-01 [PMID: 12373272] (Human)
• Jang AS, Choi IS Relationship between the perception of dyspnoea and airway inflammatory markers. Respir Med, 2002-03-01;96(3):150-4. 2002-03-01 [PMID: 11905549] (Human)
• Tangri S, Ishioka GY, Huang X, Sidney J, Southwood S, Fikes J, Sette A Structural features of peptide analogs of human histocompatibility leukocyte antigen class I epitopes that are more potent and immunogenic than wild-type peptide. J. Exp. Med., 2001-09-17;194(6):833-46. 2001-09-17 [PMID: 11560998] (Human)
• Black , K P, Merrill , K W, Jackson , S, Katz , J Cytokine profiles in parotid saliva from HIV-1-infected individuals: changes associated with opportunistic infections in the oral cavity. Oral Microbiol Immunol, 2000-04-01;15(2):74-81. 2000-04-01 [PMID: 11155169] (Human)
• Sato E, Koyama S Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity. Eur. Respir. J., 2000-11-01;16(5):951-8. 2000-11-01 [PMID: 11153598] (Human)
• Kadowaki N, Antonenko S, Lau JY, Liu YJ Natural interferon alpha/beta-producing cells link innate and adaptive immunity. J. Exp. Med., 2000-07-17;192(2):219-26. 2000-07-17 [PMID: 10899908] (Human)
• Alvarez MJ, Olaguibel JM, Garcia BE, Rodriquez A, Tabar AI, Urbiola E Airway inflammation in asthma and perennial allergic rhinitis. Relationship with nonspecific bronchial responsiveness and maximal airway narrowing. Allergy, 2000-04-01;55(4):355-62. 2000-04-01 [PMID: 10782520] (Human)
• Benson M, Strannegard IL, Wennergren G, Strannegard O Low levels of interferon-gamma in nasal fluid accompany raised levels of T-helper 2 cytokines in children with ongoing allergic rhinitis. Pediatr Allergy Immunol, 2000-02-01;11(1):20-8. 2000-02-01 [PMID: 10768732] (Human)

FAQs for IL-5 (D5000B). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL5