IL-4R alpha Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-4R alpha Antibody [Biotin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑4 R alpha
• Specificity: Detects human IL-4 R alpha in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human (rh) IL‑1 RII, rhIL-2 R beta , and rhIL‑5 R alpha is observed and less than 1% cross-reactivity with rhIL-1 RI, rhIL-2 R gamma , and rhIL-3 R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL4R
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-4R alpha Antibody [Biotin]

• CD124 antigen
• CD124
• IL-4 R alpha
• IL-4 receptor subunit alpha
• IL4R alpha
• IL-4R alpha
• IL-4R subunit alpha
• IL4R
• IL-4Ra
• IL4RACD124
• IL-4R-alpha
• interleukin 4 receptor
• interleukin-4 receptor alpha chain
• interleukin-4 receptor subunit alpha

Background

Interleukin 4 (IL‑4) is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. The biological functions of IL‑4 are mediated by the binding of IL‑4 to high‑affinity cell surface receptor complexes. Two types of IL‑4 receptor complexes have been described. The type I IL‑4 receptor complex is composed of a high‑affinity IL‑4‑binding subunit (referred to as IL‑4 R alpha ) and the common gamma chain that does not bind IL‑4 by itself. The type II IL‑4 receptor complex is composed of IL‑4 R alpha and IL‑13 R alpha 1. Besides IL‑4 signals, the type II IL‑4 receptor complex can also transduce IL‑13 signals. In the type II complex, the IL‑4 R alpha subunit binds only IL‑4 and not IL‑13. Similarly, the IL‑13 R alpha 1 subunit binds only IL‑13 and not IL‑4. The cDNA clones for both the human and mouse IL‑4 R alpha have been isolated and shown to encode an approximately 140 kDa type I transmembrane protein with a large cytoplasmic domain that is essential for signal transduction. In mouse cells, an alternatively spliced variant encoding a soluble secreted IL‑4 R alpha isoform has also been identified. Naturally occurring soluble IL‑4 R alpha that binds IL‑4 with high‑affinity has been found in mouse and human biological fluids.

1. Keegan, A.D. (2001) in Cytokine Reference, Academic Press, Vol. 1:127.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-4R alpha Antibody (BAF230)(1)

Publication using BAF230

• AB Guimarães-, NC Rochael, F Oliveira, J Echevarria, EM Saraiva Neutrophil Extracellular Traps Reprogram IL-4/GM-CSF-Induced Monocyte Differentiation to Anti-inflammatory Macrophages Front Immunol, 2017-05-17;8(0):523. 2017-05-17 [PMID: 28567039] (Western Blot, Human)

Bioinformatics

• Gene Symbol: IL4R