IL-4R alpha Antibody (129801)

• Reactivity: Mu
• Applications: WB
• Clone: 129801
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IL-4R alpha Antibody (129801) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL-4 R alpha
  Ile26-Arg233
  Accession # P16382
• Specificity: Detects mouse IL-4 R alpha in direct ELISAs and Western blots. Shows less than 1% cross‑reactivity with recombinant human IL-4 R alpha .
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: IL4R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-4R alpha Antibody (129801)

• CD124 antigen
• CD124
• IL-4 R alpha
• IL-4 receptor subunit alpha
• IL4R alpha
• IL-4R alpha
• IL-4R subunit alpha
• IL4R
• IL-4Ra
• IL4RACD124
• IL-4R-alpha
• interleukin 4 receptor
• interleukin-4 receptor alpha chain
• interleukin-4 receptor subunit alpha

Background

Interleukin 4 is a pleiotropic cytokine produced primarily by activated T cells, mast cells and basophils. The diverse biological effects of IL-4 on a variety of cell types are mediated by the binding of IL-4 to specific cell surface receptors. As is the case with many other cytokines, the functional high-affinity receptor for IL-4 is a complex consisting of a ligand binding subunit ( alpha chain) and a second subunit ( beta chain) that can modulate the ligand binding affinity of the receptor complex. It has been shown that in certain cell types, the gamma chain of the IL-2 receptor is a functional component ( beta chain) of the IL-4 receptor complex.

cDNA clones for the ligand binding chain (IL-4 R alpha ) of both the mouse and human high affinity IL-4 receptors have been isolated. The human or mouse IL-4 R alpha is an approximately 140 kDa transmembrane protein containing an extracellular domain, a transmembrane domain, and a large cytoplasmic domain that is essential for IL-4 signal transduction. In addition to the cDNA clone encoding the full-length transmembrane protein, a second cDNA clone that arises from alternate splicing and that encodes a soluble secreted form of IL-4 R alpha has been isolated from mouse cells, but not yet from human sources. A naturally-occurring soluble form of the IL-4 R alpha has also been identified in mouse biological fluids and murine cell culture supernatants.

Native or recombinant murine soluble IL-4 R alpha , as well as recombinant human soluble IL-4 R alpha , can bind IL-4 with the same affinity as the membrane bound IL-4 R alpha . Soluble IL-4 R alpha is a competitive inhibitor of IL-4 and has been shown to neutralize effectively many IL-4-mediated responses both in vivo and in vitro.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-4R alpha Antibody (MAB530)(7)

Publications using MAB530

• Shuhua He, Xiang Gu, Jintong Yang, Fei Xu, Jiachun Hu, Wei Wang et al. Sphingomyelin synthase 2 is a positive regulator of the CSF1R-STAT3 pathway in pancreatic cancer-associated macrophage Frontiers in Pharmacology 2022-10-17 [PMID: 36324684] (Western Blot, Human, Mouse)
• Shuhua He, Xiang Gu, Jintong Yang, Fei Xu, Jiachun Hu, Wei Wang et al. Sphingomyelin synthase 2 is a positive regulator of the CSF1R-STAT3 pathway in pancreatic cancer-associated macrophage Frontiers in Pharmacology 10/17/2022 [PMID: 36324684]
• Multivalent Targeting Based Delivery of Therapeutic Peptide using AP1-ELP Carrier for Effective Cancer Therapy Theranostics, 2016-09-25;6(12):2235-2249. 2016-09-25 [PMID: 27924160] (IHC-P, Mouse)
• ES Kim, YE Choi, SJ Hwang, YH Han, MJ Park, IH Bae IL-4, a direct target of miR-340/429, is involved in radiation-induced aggressive tumor behavior in human carcinoma cells Oncotarget, 2016-12-27;7(52):86836-86856. 2016-12-27 [PMID: 27895317] (Western Blot, Human)
• XIN LIU, JINYUAN MAO, CHENG HAN, SHIQIAO PENG, CHENYAN LI, TING JIN et al. CXCR4 antagonist AMD3100 ameliorates thyroid damage in autoimmune thyroiditis in NOD.H-2h4 mice Molecular Medicine Reports 4/1/2016 [PMID: 26935473]
• XIN LIU, JINYUAN MAO, CHENG HAN, SHIQIAO PENG, CHENYAN LI, TING JIN et al. CXCR4 antagonist AMD3100 ameliorates thyroid damage in autoimmune thyroiditis in NOD.H-2h4 mice Molecular Medicine Reports 2016-04-01 [PMID: 26935473] (Immunohistochemistry, Mouse)
• Hiromura Y, Kishida T, Nakano H, Hama T, Imanishi J, Hisa Y, Mazda O IL-21 administration into the nostril alleviates murine allergic rhinitis. J. Immunol., 2007-11-15;179(10):7157-65. 2007-11-15 [PMID: 17982108] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: IL4R
• Uniprot:
  • P16382()