Recombinant Human IL-4 Protein

SDS-Page: Recombinant Mouse IL-4 Protein [NBP2-52393] - 15% SDS Page (3 ug)

Product Discontinued

Recombinant Human IL-4 Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 25-153 of Human IL-4
  
  

  Source: E.coli

  Amino Acid Sequence: MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS

• Details of Functionality: Human IL-4 stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range is less than or equal to 0.6 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL4
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 15 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human IL-4 Protein

• B cell growth factor 1
• BCDF
• B-cell stimulatory factor 1
• BCGF1
• BCGF-1
• binetrakin
• BSF1
• BSF-1
• IL4
• IL-4
• IL-4B_cell stimulatory factor 1
• IL4E12
• interleukin 4
• interleukin-4
• Lymphocyte stimulatory factor 1
• MGC79402
• pitrakinra

Background

IL-4 (Interleukin 4, B cell stimulatory factor) is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. IL-4 has many different functions including promoting proliferation and differentiation of immunologically competent cells. One of its main functions is regulating differentiation of naive CD4+ T cells into helper Th2 cells, the other main function is regulating B cells IgE and IgG1 production. Human IL-4 shows 50% homology with mouse IL-4, but its activities are species-specific and human IL-4 shows no activity on murine and rat cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for IL-4 Recombinant Protein (NBP2-52393)(1)

Publication using NBP2-52393

• Shimizu S, Yonezawa K, Haruna M et al. Relationship between facial skin problems with a focus on inflammatory cytokines and the presence of Malassezia in 1-month-old infants Scientific reports 2023-03-28 [PMID: 36977695] (WB, Human)

Bioinformatics

• Gene Symbol: IL4