Recombinant Human IL-4 Protein

Product Discontinued

Recombinant Human IL-4 Protein Summary

• Description: Recombinant protein corresponding to amino acids His 25-Ser 153 of mature human IL-4. Accession #: AF 395008.

  Source: E. coli

• Details of Functionality: The ED50 measured in a TF-1 proliferation assay is typically 100 pg/ml, corresponding to a specific activity of approximately 1x10^7Units/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL4
• Purity: >95% pure by SDS-PAGE
• Endotoxin Note: Less than 0.01 ng/ug cytokine as determined by the LAL assay.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • ELISA
  • Immunoassay
• Application Notes: This recombinant human IL-4 has been tested by sandwich ELISA using anti-human IL-4 antibody pair (8D4-8/MP4-25D2).
• Theoretical MW: 14.96 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95% pure by SDS-PAGE

Alternate Names for Recombinant Human IL-4 Protein

• B cell growth factor 1
• BCDF
• B-cell stimulatory factor 1
• BCGF1
• BCGF-1
• binetrakin
• BSF1
• BSF-1
• IL4
• IL-4
• IL-4B_cell stimulatory factor 1
• IL4E12
• interleukin 4
• interleukin-4
• Lymphocyte stimulatory factor 1
• MGC79402
• pitrakinra

Background

Human IL-4 is a ~15-19 kDa factor also known as BCDF, BCGF-1 and BSF-1. IL-4 is secreted by activated Th2 and natural killer T cells and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4 in conjunction with other signals such as CD40 induces Ig isotype switching of B cells and upregulation of surface antigens including MHC class II and CD23.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL4