Rat IL-4 ELISA Kit (Colorimetric)

ELISA: IL-4 ELISA Kit [Biotin] [NBP1-92700] - Standard curve

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum
• Standard Curve Range: 1.6 - 100 pg/mL
• Sensitivity: 0.2 pg/mL

Rat IL-4 ELISA Kit (Colorimetric) Summary

• Description: This kit is an enzyme-linked immunosorbent assay for quantitative detection.
• Specificity: The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a rat IL-4 positive serum. There was no crossreactivity detected.
• Standard Curve Range: 1.6 - 100 pg/mL
• Sensitivity: 0.2 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 10%
• Intra-Assay: CV% < 5.0%
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL4

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat IL-4 ELISA Kit (Colorimetric)

• B cell growth factor 1
• BCDF
• B-cell stimulatory factor 1
• BCGF1
• BCGF-1
• binetrakin
• BSF1
• BSF-1
• IL4
• IL-4
• IL-4B_cell stimulatory factor 1
• IL4E12
• interleukin 4
• interleukin-4
• Lymphocyte stimulatory factor 1
• MGC79402
• pitrakinra

Background

IL-4 mediates its function by binding to receptors expressed on target cells. The IL-4 receptors exhibit an affinity of approximately 10-10 M. Receptors exist on freshly prepared B and T lymphocytes and macrophages, as well as on various cell lines including lymphoid cells, mast cell lines, a variety of other hematopoietic cell lines, fibroblasts and stromal cell lines. On T and B cells, receptors are present in low numbers (appr. 400), which are reported to be upregulated by IL-2 and IL-4. IL-4 is produced by a particular subset of T helper cells, the TH2 cells.These cells tend to make a specific set of lymphokines including IL-4, IL-5, IL-6, IL-10, IL-13 and GM-CSF and fail to produce IL-2, IFN-gamma, and lymphotoxin (TNF-beta). Apart from T cells, it has been shown that mast cells can produce IL-4. IL-4 exerts numerous effects on various hematopoietic cell types. On B cells, IL-4 promotes immunological class switching to IgE and IgG1 isotypes and upregulates MHC class II and CD23 expression. It can promote survival, growth, and differentiation of both T and B lymphocytes, mast cells, and endothelial cells. In addition, IL-4 can inhibit the production of TNF, IL-1, and IL-6 by macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IL4