IL-38/IL-1F10 Antibody (798036R) [Unconjugated]

HAF005). A specific band was detected for IL-38/IL-1F10 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF005). A specific band was detected for IL-38/IL-1F10 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of mouse skin tissue. PVDF membrane was probed with 2 µg/mL of Recombinant Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB7774R) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-38/IL-1F10 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of mouse skin tissue. PVDF membrane was probed with 2 µg/mL of Recombinant Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB7774R) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

MAB006, open histogram), followed by Fluorescein-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0104B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." > MAB006, open histogram), followed by Fluorescein-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0104B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." title="RAW 264.7 mouse monocyte/macrophage cell line was stained with Recombinant Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB7774R, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Fluorescein-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0104B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." />

RAW 264.7 mouse monocyte/macrophage cell line was stained with Recombinant Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB7774R, filled histogram) or isotype control antibody (Catalog # <a class=

MAB77742) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB7774R) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." > MAB77742) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB7774R) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." title="Recombinant Mouse IL-38/IL-1F10 protein was serially diluted 2-fold and captured by Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB77742) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB7774R) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." />

Recombinant Mouse IL-38/IL-1F10 protein was serially diluted 2-fold and captured by Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # <a class=

• Reactivity: Mu
• Applications: WB, ELISA, Flow
• Clone: 798036R
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IL-38/IL-1F10 Antibody (798036R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: E.coli-derived recombinant mouse IL-38/IL-1F10
  Met1-Arg152
  Accession # Q8R459
• Specificity: Detects mouse IL-38/IL-1F10 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Il1f10
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-38/IL-1F10 Antibody (798036R) [Unconjugated]

• FIL1- theta
• FKSG75
• IL1F10
• IL-1HY2
• IL38
• IL-38
• interleukin 1 family, member 10 (theta)
• UNQ6119/PRO20041

Background

IL-1F10, also known as IL-1HY2, is a secreted protein that shares sequence and structural similarity with IL-1 receptor antagonist and other IL-1 family members. It is expressed in basal epithelia of the skin and in proliferating germinal center CD20⁺ B cells. IL-1F10 binds soluble IL-1RI in vitro and may participate in regulation of immune responses. Mouse IL-1F10 shares 82% and 93% aa identity with human and rat IL-1F10, respectively. IL-1F10 is predicted to be 17 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Il1f10
• Uniprot:
  • Q8R459()