IL-38/IL-1F10 Antibody (316709)

• Reactivity: Hu
• Applications: WB
• Clone: 316709
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-38/IL-1F10 Antibody (316709) Summary

• Immunogen: E. coli-derived recombinant human IL‑38/IL‑1F10
  Met1-Trp152
  Accession # Q8WWZ1
• Specificity: Detects human IL‑38/IL‑1F10 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse IL-1F10 is observed and no cross-reactivity with recombinant human (rh) IL-1 alpha /IL-1F1 rhIL-1 beta /IL-1F2, rhIL-1ra/IL-1F3, rhIL-18/IL-1F4, rhIL-36Ra/IL‑1F5, rhIL-36 alpha /IL‑1F6, rhIL‑1F7/FIL1 zeta , rhIL-36 beta /IL-1F8, or rhIL-36 gamma /IL‑1F9 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: IL1F10
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-38/IL-1F10 Antibody (316709)

• FIL1- theta
• FKSG75
• IL1F10
• IL-1HY2
• IL38
• IL-38
• interleukin 1 family, member 10 (theta)
• UNQ6119/PRO20041

Background

Human IL-38, previously called IL-1F10 or IL-1HY2, is a secreted cytokine belonging to the IL-1 family. It is synthesized as a 152 amino acid mature protein that lacks an identifiable signal sequence. It binds to soluble IL-1 RI. Human IL-38/IL-1F10 shares 82% aa sequence identity with mouse  IL-38/IL-1F10. Human  IL-38/IL-1F10 also shares 39% and 27% aa sequence identity with IL-1ra/IL-1F3 and IL-1 beta /IL-1F2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-38/IL-1F10 Antibody (MAB2427)(1)

Publication using MAB2427

• Southcombe J, Redman C, Sargent I, Granne I Interleukin-1 family cytokines and their regulatory proteins in normal pregnancy and pre-eclampsia. Clin Exp Immunol, 2015-06-29;181(3):480-90. 2015-06-29 [PMID: 25693732] (Western Blot, Human)

Bioinformatics

• Gene Symbol: IL1F10
• Uniprot:
  • Q8WWZ1()