Western blot shows lysates of human peripheral blood mononuclear cells (PBMCs) untreated (-) or treated (+) with 2 μg/mL LPS for 24 hours and THP‑1 human acute monocytic leukemia cell line untreated (-) or ...read more
E. coli-derived recombinant human IL-37/IL-1F7 Lys27-Asp192 Accession # Q9NZH6-2
Detects human IL-37/IL-1F7 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with recombinant human (rh) FIL1δ, rhFIL1 epsilon, rhFIL1 eta, rhIL-1 alpha, rhIL-1 beta, rhIL-18, and rhIL-1 H1 is observed.
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for IL-37/IL-1F7 Antibody [Unconjugated]
IL1H4IL1F7 (canonical product IL-1F7b)
IL-1H4IL-1F7b (IL-1H4, IL-1H, IL-1RP1)
IL1RP1interleukin 1 family member 7
interleukin 1 family, member 7 (zeta)
interleukin 1, zeta
interleukin-1 family member 7
Interleukin-1 homolog 4
interleukin-1 superfamily z
Human interleukin 1 family member 7 (IL-1F7), also named FIL-1Z, IL-1H4, and IL-1RP1, belongs to the IL-1 cytokine family, which currently has ten members. With the exception of IL-18 that maps to human chromosome 11, all other IL-1 family members map to the same cluster on human chromosome 2. Five alternatively spliced transcripts that arise through alternate exon usage have been described. These transcripts encode five different IL-1F7 isoforms (IL-1F7a through e also referred to as isofoms 1 through 5) that have distinct expression profiles. Polymorphism in the protein sequence of IL-1F7 isoforms also exists. Like IL-1 alpha, IL-1 beta and IL-18, all of the IL-1F7 variants lack a typical signal peptide. The longest IL-1F7 transcript, referred to as IL-1F7b or IL-1F7 isoform 1, encodes a 218 amino acid (aa) residues proprotein containing a 45 aa propeptide, which is removed by caspase-1 to generate the 173 aa mature segment. Mature IL-1F7b and other IL-1F7 variants lack potential N-linked glycosylation sites. The secreted mature IL-17F7b was reported to exist as a nondisulfide linked homodimers in solution, IL-1F7 shares approximately 21%, 24%, and 30% aa sequence identity with mature IL-1 alpha, IL-1 beta and IL-1ra, respectively. Mouse IL-1F7 has not been reported, but human IL-1F7 is active on mouse cells. IL-1F7b binds to IL-18 R alpha with low affinity but does not exert any IL-18 agonistic or antagonistic effects. IL-1F7b also binds to the IL-18BP to enhance the antagonistic effects of IL-18BP. It has been proposed that IL-1F7b form a trimeric complex with IL-18BP and IL-18 R beta. This complex blocks IL-18 activity by sequestering the signal transducing subunit and preventing it from participating in IL-18 signaling (1‑8).
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Kumar S. et al. (2000) J. Biol. Chem. 275:10308.
Busfield S.J. et al. (2000) Genomics 66:213.
Kumar S. et al. (2002) Cytokine 18:61.
Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:13723.
Gao, W. et al. (2003) J. Immunol. 170:1077.
Pan, G. et al. (2001) Cytokine 13:1.
Sims, J.E. et al. (2001) Trends Immunol. 22:533.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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