IL-33 Antibody [Unconjugated]

Recombinant Human IL-33 (3625-IL) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human IL-33 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3625). The ND50 is typically ≤ 3.0 µg/mL.

IL-33 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3625) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

IL-33 mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope® Probe (Catalog # 400111) and stained using ACD RNAscope® 2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Goat Anti-Human IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3625) at 3 ug/mL with overnight incubation at 4 °C followed by incubation with the Anti-Goat IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC004) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).

• Reactivity: Hu
• Applications: WB, IHC, B/N, Dual ISH-IHC, ELISA(Cap), ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-33 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IL-33
  Ser112-Thr270
  Accession # O95760
• Specificity: Detects human IL-33 in ELISAs and Western blots. In sandwich ELISAs, less than 0.05% cross-reactivity with recombinant mouse (rm) IL‑33 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL33
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Dual RNAscope ISH-IHC 3-25 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization ≤ 3.0 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human IL-33 Antibody (Catalog # AF3625)
  ELISA Detection: Human IL-33 Biotinylated Antibody (Catalog # BAF3625)
  Standard:Recombinant Human IL-33 (Catalog # 3625-IL)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-33 Antibody [Unconjugated]

• C9orf26
• C9orf26chromosome 9 open reading frame 26 (NF-HEV)
• DKFZp586H0523
• DVS27
• DVS27-related protein
• IL1F11
• IL-1F11
• IL33
• IL-33
• interleukin 33
• Interleukin-1 family member 11
• interleukin-33
• NFHEV
• NF-HEV
• NF-HEVNFEHEV
• Nuclear factor from high endothelial venules
• RP11-575C20.2

Background

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1‑3). DVS 27 was identifed as a gene that is up‑regulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is up‑regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature human IL-33 share 52‑58% aa sequence identity with mouse and rat IL-33. Human IL-33 shares less than 20% aa sequence identity with other IL-1 family proteins.

1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
3. Schmitz, J. et al. (2005) Immunity 23:479.
4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930.
7. Dinarello, C.A. (2005) Immunity 23:461.
8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-33 Antibody (AF3625)(53)

Publications using AF3625

• Donahue, KL;Watkoske, HR;Kadiyala, P;Du, W;Brown, K;Scales, MK;Elhossiny, AM;Espinoza, CE;Lasse Opsahl, EL;Griffith, BD;Wen, Y;Sun, L;Velez-Delgado, A;Renollet, NM;Morales, J;Nedzesky, NM;Baliira, RK;Menjivar, RE;Medina-Cabrera, PI;Rao, A;Allen, B;Shi, J;Frankel, TL;Carpenter, ES;Bednar, F;Zhang, Y;Pasca di Magliano, M; Oncogenic KRAS-dependent stromal interleukin-33 directs the pancreatic microenvironment to promote tumor growth Cancer discovery 2024-07-03 [PMID: 38958646] (Immunohistochemistry, Western Blot, Mouse, Transgenic Mouse)
• Raphael, HE;Hassan, GF;Osorio, OA;Cohen, LS;Payne, MD;Katz-Kiriakos, E;Tata, I;Hicks, J;Byers, DE;Zhang, B;Alexander-Brett, J; Activator protein transcription factors coordinate human IL-33 expression from noncanonical promoters in chronic airway disease JCI insight 2024-03-08 [PMID: 38456508] (Western Blot, Human)
• Jeon, YJ;Gil, CH;Won, J;Jo, A;Kim, HJ; Symbiotic microbiome Staphylococcus epidermidis restricts IL-33 production in allergic nasal epithelium via limiting the cellular necroptosis BMC microbiology 2023-05-26 [PMID: 37237381] (Western Blot, Mouse)
• KE Stephenson, J Porte, A Kelly, WA Wallace, CE Huntington, CL Overed-Say, ES Cohen, RG Jenkins, AE John The IL-33:ST2 axis is unlikely to play a central fibrogenic role in idiopathic pulmonary fibrosis Respiratory Research, 2023-03-23;24(1):89. 3/23/2023 [PMID: 36949463]
• Yao F, Luo Y, Liu YC et al. Imatinib inhibits pericyte-fibroblast transition and inflammation and promotes axon regeneration by blocking the PDGF-BB/PDGFR? pathway in spinal cord injury Inflammation and Regeneration 2022-09-26 [PMID: 36163271]
• GM Jowett, E Read, LB Roberts, D Coman, M Vilà Gonzá, T Zabinski, U Niazi, R Reis, TJ Trieu, D Danovi, E Gentleman, L Vallier, MA Curtis, GM Lord, JF Neves Organoids capture tissue-specific innate lymphoid cell development in mice and humans Cell Reports, 2022-08-30;40(9):111281. 2022-08-30 [PMID: 36044863] (Neutralization, Human)
• Rajia Bahri, Orsolya Kiss, Ian Prise, Karen M. Garcia-Rodriguez, Haris Atmoko, Julia M. Martínez-Gómez et al. Human Melanoma-Associated Mast Cells Display a Distinct Transcriptional Signature Characterized by an Upregulation of the Complement Component 3 That Correlates With Poor Prognosis Frontiers in Immunology 2022-05-20 [PMID: 35669782]
• Aftab Alam, Eric Levanduski, Parker Denz, Helena Solleiro Villavicencio, Maulasri Bhatta, Lamees Alhorebi et al. Fungal mycobiome drives IL-33 secretion and type 2 immunity in pancreatic cancer Cancer Cell 2022-02-01 [PMID: 35120601]
• H Tsuda, SI Tominaga, M Ohtsuki, M Komine Nuclear IL-33 regulates cytokinesis and cell motility in normal human epidermal keratinocytes Journal of dermatological science, 2022-01-25;0(0):. 2022-01-25 [PMID: 35105507] (Western Blot, Human)
• Meng X, Qian X, Ding X et al. Eosinophils regulate intra-adipose axonal plasticity Proceedings of the National Academy of Sciences of the United States of America 1/18/2022 [PMID: 35042776]
• JE Miller, H Lingegowda, LK Symons, O Bougie, SL Young, BA Lessey, M Koti, C Tayade Interleukin-33 activates group 2 innate lymphoid cell expansion and modulates endometriosis JCI Insight, 2021-12-08;0(0):. 2021-12-08 [PMID: 34699382] (IF, Human)
• BJ Mehrara, HJ Park, RP Kataru, J Bromberg, M Coriddi, JE Baik, J Shin, C Li, MR Cavalli, EM Encarnacio, M Lee, KJ Van Zee, E Riedel, JH Dayan Pilot Study of Anti-Th2 Immunotherapy for the Treatment of Breast Cancer-Related Upper Extremity Lymphedema Biology, 2021-09-18;10(9):. 2021-09-18 [PMID: 34571811] (IHC, Human)
• TR Mikuls, R Gaurav, GM Thiele, BR England, MG Wolfe, BP Shaw, KL Bailey, TA Wyatt, AJ Nelson, MJ Duryee, CD Hunter, D Wang, DJ Romberger, DP Ascherman, JA Poole The impact of airborne endotoxin exposure on rheumatoid arthritis-related joint damage, autoantigen expression, autoimmunity, and lung disease International immunopharmacology, 2021-08-27;100(0):108069. 2021-08-27 [PMID: 34461491] (IHC, Human)
• Rohit Gaurav, Daniel R. Anderson, Stanley J. Radio, Kristina L. Bailey, Bryant R. England, Ted R. Mikuls et al. IL-33 Depletion in COVID-19 Lungs Chest 2021-11-01 [PMID: 34245743] (Immunohistochemistry, Human)
• G Ercolano, A Gomez-Cade, N Dumauthioz, G Vanoni, M Kreutzfeld, T Wyss, L Michalik, R Loyon, A Ianaro, PC Ho, C Borg, M Kopf, D Merkler, P Krebs, P Romero, S Trabanelli, C Jandus PPAR? drives IL-33-dependent ILC2 pro-tumoral functions Nature Communications, 2021-05-05;12(1):2538. 2021-05-05 [PMID: 33953160] (IHC, Human)
• Federico Perez, Carolina N. Ruera, Emanuel Miculan, Paula Carasi, Karen Dubois-Camacho, Laura Garbi et al. IL-33 Alarmin and Its Active Proinflammatory Fragments Are Released in Small Intestine in Celiac Disease Frontiers in Immunology 2020-10-08 [PMID: 33133101]
• S Yangngam, S Thongchot, A Pongpaibul, K Vaeteewoot, S Pinlaor, P Thuwajit, S Okada, MA Hermoso, C Thuwajit High level of interleukin-33 in cancer cells and cancer-associated fibroblasts correlates with good prognosis and suppressed migration in cholangiocarcinoma J Cancer, 2020-09-23;11(22):6571-6581. 2020-09-23 [PMID: 33046978] (IHC, Western Blot, Human)
• YJ Jeon, CH Gil, J Won, A Jo, HJ Kim Symbiotic microbiome Staphylococcus aureus from human nasal mucus modulates IL-33-mediated type 2 immune responses in allergic nasal mucosa BMC Microbiol, 2020-10-07;20(1):301. 2020-10-07 [PMID: 33028252] (Western Blot, Human)
• Ophir Shani, Tatiana Vorobyov, Lea Monteran, Dor Lavie, Noam Cohen, Yael Raz et al. Fibroblast-derived IL-33 facilitates breast cancer metastasis by modifying the immune microenvironment and driving type-2 immunity Cancer Research 2020-12-01 [PMID: 33023944]
• Yuning Lu, Gemma Basatemur, Ian C. Scott, Davide Chiarugi, Marc Clement, James Harrison et al. Interleukin-33 Signaling Controls the Development of Iron-Recycling Macrophages Immunity 2020-05-19 [PMID: 32272082]
• Suresh Govatati, Prahalathan Pichavaram, Jagadeesh Janjanam, Liang Guo, Renu Virmani, Gadiparthi N. Rao Myristoylation of LMCD1 Leads to Its Species-Specific Derepression of E2F1 and NFATc1 in the Modulation of CDC6 and IL-33 Expression During Development of Vascular Lesions Arteriosclerosis, Thrombosis, and Vascular Biology 2020-05-01 [PMID: 32160773] (Neutralization, Human)
• JA Moral, J Leung, LA Rojas, J Ruan, J Zhao, Z Sethna, A Ramnarain, B Gasmi, M Gururajan, D Redmond, G Askan, U Bhanot, E Elyada, Y Park, DA Tuveson, M Gönen, SD Leach, JD Wolchok, RP DeMatteo, T Merghoub, VP Balachandr ILC2s amplify PD-1 blockade by activating tissue-specific cancer immunity Nature, 2020-02-19;0(0):. 2020-02-19 [PMID: 32076273] (IHC, Mouse)
• JF Zhang, T Tao, K Wang, GX Zhang, Y Yan, HR Lin, Y Li, MW Guan, JJ Yu, XD Wang IL-33/ST2 axis promotes glioblastoma cell invasion by accumulating tenascin-C Sci Rep, 2019-12-30;9(1):20276. 2019-12-30 [PMID: 31889095] (IHC, Human)
• D Izadi, TB Layton, L Williams, F McCann, M Cabrita, AI Espirito S, W Xie, M Fritzsche, H Colin-York, M Feldmann, KS Midwood, J Nanchahal Identification of TNFR2 and IL-33 as therapeutic targets in localized fibrosis Sci Adv, 2019-12-04;5(12):eaay0370. 2019-12-04 [PMID: 31840071] (ICC, Human)
• Q Zhou, X Wu, X Wang, Z Yu, T Pan, Z Li, X Chang, Z Jin, J Li, Z Zhu, B Liu, L Su The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-alpha/IL-33/ST2L signaling promotes gastric cancer metastasis Oncogene, 2019-10-28;0(0):. 2019-10-28 [PMID: 31659258] (Cell Culture, Human)
• Glauben Landskron, Marjorie De la Fuente López, Karen Dubois-Camacho, David Díaz-Jiménez, Octavio Orellana-Serradell, Diego Romero et al. Interleukin 33/ST2 Axis Components Are Associated to Desmoplasia, a Metastasis-Related Factor in Colorectal Cancer Frontiers in Immunology 2019-06-21 [PMID: 31281317]
• AL Frisbee, MM Saleh, MK Young, JL Leslie, ME Simpson, MM Abhyankar, CA Cowardin, JZ Ma, P Pramoonjag, SD Turner, AP Liou, EL Buonomo, WA Petri IL-33 drives group 2 innate lymphoid cell-mediated protection during Clostridium difficile infection Nat Commun, 2019-06-20;10(1):2712. 2019-06-20 [PMID: 31221971] (IHC, Human)
• Suresh Govatati, Prahalathan Pichavaram, Jagadeesh Janjanam, Baolin Zhang, Nikhlesh K. Singh, Arul M. Mani et al. NFATc1-E2F1-LMCD1–Mediated IL-33 Expression by Thrombin Is Required for Injury-Induced Neointima Formation Arteriosclerosis, Thrombosis, and Vascular Biology 2019-06-01 [PMID: 31043075]
• Nataliya Razumilava, Junya Shiota, Nureen H. Mohamad Zaki, Ramon Ocadiz‐Ruiz, Christine M. Cieslak, Kais Zakharia et al. Hedgehog Signaling Modulates Interleukin‐33‐Dependent Extrahepatic Bile Duct Cell Proliferation in Mice Hepatology Communications 2019-02-01 [PMID: 30766964]
• G Cui, J Ren, G Xu, Z Li, W Zheng, A Yuan Cellular and clinicopathological features of the IL-33/ST2 axis in human esophageal squamous cell carcinomas Cancer Cell Int., 2018-12-11;18(0):203. 2018-12-11 [PMID: 30559604] (IHC, Human)
• Guido Carpino, Vincenzo Cardinale, Trine Folseraas, Diletta Overi, Krzysztof Grzyb, Daniele Costantini et al. Neoplastic Transformation of the Peribiliary Stem Cell Niche in Cholangiocarcinoma Arisen in Primary Sclerosing Cholangitis Hepatology 2019-02-01 [PMID: 30102768]
• N Gour, S Lajoie, U Smole, M White, D Hu, P Goddard, S Huntsman, C Eng, A Mak, S Oh, JH Kim, A Sharma, S Plante, IH Salem, Y Resch, X Xiao, N Yao, A Singh, S Vrtala, J Chakir, EG Burchard, AP Lane, M Wills-Karp Dysregulated invertebrate tropomyosin-dectin-1 interaction confers susceptibility to allergic diseases Sci Immunol, 2018-02-23;3(20):. 2018-02-23 [PMID: 29475849] (ELISA Capture, Human, Mouse)
• Megan Osbourn, Dinesh C. Soares, Francesco Vacca, E. Suzanne Cohen, Ian C. Scott, William F. Gregory et al. HpARI Protein Secreted by a Helminth Parasite Suppresses Interleukin-33 Immunity 2017-10-17 [PMID: 29045903]
• Zhengxiang He, Lili Chen, Fabricio O. Souto, Claudia Canasto-Chibuque, Gerold Bongers, Madhura Deshpande et al. Epithelial-derived IL-33 promotes intestinal tumorigenesis in Apc Min/+ mice Scientific Reports 2017-07-14 [PMID: 28710436]
• Stephanie Elizabeth Johanna Zandee, Richard Anthony O'Connor, Iris Mair, Melanie Dawn Leech, Anna Williams, Stephen Mark Anderton IL‐10‐producing, ST2‐expressing Foxp3 + T cells in multiple sclerosis brain lesions Immunology & Cell Biology 2017-05-01 [PMID: 28169287]
• Interleukin-33 and RANK-L Interplay in the Alveolar Bone Loss Associated to Periodontitis PLoS ONE, 2016-12-19;11(12):e0168080. 2016-12-19 [PMID: 27992569] (IHC-P, Mouse)
• Uffe Nygaard, Ellen H van den Bogaard, Hanna Niehues, Malene Hvid, Mette Deleuran, Claus Johansen et al. The ”Alarmins” HMBG1 and IL-33 Downregulate Structural Skin Barrier Proteins and Impair Epidermal Growth Acta Dermato Venereologica 2017-03-10 [PMID: 27868148]
• Mona Dixon Gundersen, Rasmus Goll, Johanna Hol, Trine Olsen, Renathe Rismo, Sveinung W. Sørbye et al. Loss of interleukin 33 expression in colonic crypts - a potential marker for disease remission in ulcerative colitis Scientific Reports 2016-10-17 [PMID: 27748438]
• TLR2 Activation Limits Rhinovirus-Stimulated CXCL-10 by Attenuating IRAK-1-Dependent IL-33 Receptor Signaling in Human Bronchial Epithelial Cells J Immunol, 2016-08-08;0(0):. 2016-08-08 [PMID: 27503209] (Neutralization, Human)
• DOROTHEE GRAMATZKI, KARL FREI, GIERI CATHOMAS, HOLGER MOCH, MICHAEL WELLER, KIRSTEN DIANA MERTZ Interleukin-33 in human gliomas: Expression and prognostic significance Oncology Letters 2016-07-01 [PMID: 27347163]
• YAN YANG, JUN-BIN WANG, YU-MEI LI, Y U ZHAO, RUI WANG, QIONG WU et al. Role of IL-33 expression in oncogenesis and development of human hepatocellular carcinoma Oncology Letters 2016-07-01 [PMID: 27347162]
• Kirsten D. Mertz, Lukas F. Mager, Marie-Hélène Wasmer, Thore Thiesler, Viktor H. Koelzer, Giulia Ruzzante et al. The IL-33/ST2 pathway contributes to intestinal tumorigenesis in humans and mice OncoImmunology 2016-01-02 [PMID: 26942077]
• E. Suzanne Cohen, Ian C. Scott, Jayesh B. Majithiya, Laura Rapley, Benjamin P. Kemp, Elizabeth England et al. Oxidation of the alarmin IL-33 regulates ST2-dependent inflammation Nature Communications 2015-09-14 [PMID: 26365875]
• Amanda Waddell, Jefferson E. Vallance, Preston D. Moore, Amy T. Hummel, David Wu, Shiva K. Shanmukhappa et al. IL-33 Signaling Protects from Murine Oxazolone Colitis by Supporting Intestinal Epithelial Function Inflammatory Bowel Diseases 2015-12-01 [PMID: 26313694] (Immunohistochemistry, Human)
• Mager L, Riether C, Schurch C, Banz Y, Wasmer M, Stuber R, Theocharides A, Li X, Xia Y, Saito H, Nakae S, Baerlocher G, Manz M, McCoy K, Macpherson A, Ochsenbein A, Beutler B, Krebs P IL-33 signaling contributes to the pathogenesis of myeloproliferative neoplasms. J Clin Invest, 2015-05-26;125(7):2579-91. 2015-05-26 [PMID: 26011644] (IHC-P, Human)
• Sundnes O, Pietka W, Loos T, Sponheim J, Rankin A, Pflanz S, Bertelsen V, Sitek J, Hol J, Haraldsen G, Khnykin D Epidermal Expression and Regulation of Interleukin-33 during Homeostasis and Inflammation: Strong Species Differences. J Invest Dermatol, 2015-03-04;135(7):1771-80. 2015-03-04 [PMID: 25739051] (IHC-P, Human)
• Mamdouh A K Sedhom, Mélanie Pichery, Jenna R Murdoch, Benoit Foligné, Nathalie Ortega, Sylvain Normand et al. Neutralisation of the interleukin-33/ST2 pathway ameliorates experimental colitis through enhancement of mucosal healing in mice Gut 2013-12-01 [PMID: 23172891]
• Kamekura R, Kojima T, Takano K, Go M, Sawada N, Himi T The role of IL-33 and its receptor ST2 in human nasal epithelium with allergic rhinitis. Clin. Exp. Allergy, 2012-02-01;42(2):218-28. 2012-02-01 [PMID: 22233535] (Neutralization, Human)
• Hong YS, Moon SJ, Moon S, Joo YB, Joo Y, Jeon CH, Jeon C, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis. J Korean Med Sci, 2011-09-01;26(9):1132-9. 2011-09-01 [PMID: 21935266] (ELISA Development, IHC-P, Human)
• Byrne SN, Beaugie C, O'Sullivan C, Leighton S, Halliday GM The immune-modulating cytokine and endogenous Alarmin interleukin-33 is upregulated in skin exposed to inflammatory UVB radiation. Am. J. Pathol., 2011-05-13;179(1):211-22. 2011-05-13 [PMID: 21703403] (IHC-Fr, Human)
• Dominique Talabot-Ayer, Thomas McKee, Patrizia Gindre, Sylvette Bas, Dominique L. Baeten, Cem Gabay et al. Distinct serum and synovial fluid interleukin (IL)-33 levels in rheumatoid arthritis, psoriatic arthritis and osteoarthritis Joint Bone Spine 2012-01-01 [PMID: 21441054]
• Nishida A, Andoh A, Imaeda H, Inatomi O, Shiomi H, Fujiyama Y Expression of interleukin 1-like cytokine interleukin 33 and its receptor complex (ST2L and IL1RAcP) in human pancreatic myofibroblasts. Gut, 2009-12-08;59(4):531-41. 2009-12-08 [PMID: 19996325] (IHC-P, Western Blot, Human)
• Palmer G, Talabot-Ayer D, Lamacchia C, Toy D, Seemayer CA, Viatte S, Finckh A, Smith DE, Gabay C Inhibition of interleukin-33 signaling attenuates the severity of experimental arthritis. Arthritis Rheum., 2009-03-01;60(3):738-49. 2009-03-01 [PMID: 19248109] (IHC-P, Human)

Review for IL-33 Antibody (AF3625) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 01/08/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: See PMID 23172891
• Species: Human

Bioinformatics

• Gene Symbol: IL33
• Uniprot:
  • O95760()