IL-33 Antibody (396118) [Phycoerythrin]

bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse IL-33 PE-conjugated Mono-clonal Antibody (Catalog # IC3626P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Mu
• Applications: Flow
• Clone: 396118
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

IL-33 Antibody (396118) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant mouse IL-33
  Ser109-Ile266
  Accession # Q8BVZ5
• Specificity: Detects mouse IL-33 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL33
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-33 Antibody (396118) [Phycoerythrin]

• C9orf26
• C9orf26chromosome 9 open reading frame 26 (NF-HEV)
• DKFZp586H0523
• DVS27
• DVS27-related protein
• IL1F11
• IL-1F11
• IL33
• IL-33
• interleukin 33
• Interleukin-1 family member 11
• interleukin-33
• NFHEV
• NF-HEV
• NF-HEVNFEHEV
• Nuclear factor from high endothelial venules
• RP11-575C20.2

Background

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1-3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% amino acid (aa) sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.

1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
3. Schmitz, J. et al. (2005) Immunity 23:479.
4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930.
7. Dinarello, C.A. (2005) Immunity 23:461.
8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-33 Antibody (IC3626P)(8)

Publications using IC3626P

• M Yamaguchi, SK Samuchiwal, O Quehenberg, JA Boyce, B Balestrier Macrophages regulate lung ILC2 activation via Pla2g5-dependent mechanisms Mucosal Immunol, 2017-12-20;11(3):615-626. 2017-12-20 [PMID: 29346348] (Flow Cytometry, Mouse)
• S Chen, B Chen, Z Wen, Z Huang, L Ye IL-33/ST2-mediated inflammation in macrophages is directly abrogated by IL-10 during rheumatoid arthritis Oncotarget, 2017-05-16;8(20):32407-32418. 2017-05-16 [PMID: 28415811] (Flow Cytometry, ICC, Mouse)
• Kempuraj D, Thangavel R, Yang E, Pattani S, Zaheer S, Santillan D, Santillan M, Zaheer A Dopaminergic Toxin 1-Methyl-4-Phenylpyridinium, Proteins alpha-Synuclein and Glia Maturation Factor Activate Mast Cells and Release Inflammatory Mediators. PLoS ONE, 2015-08-14;10(8):e0135776. 2015-08-14 [PMID: 26275153] (Flow Cytometry, Mouse)
• Verma , A, Kroetz , D N, Tweedle , J L, Deepe , G S Jr Type II cytokines impair host defense against an intracellular fungal pathogen by amplifying macrophage generation of IL-33. Mucosal Immunol, 2014-08-13;8(2):380-9. 2014-08-13 [PMID: 25118166] (Flow Cytometry, Mouse)
• Guabiraba R, Besnard A, Menezes G, Secher T, Jabir M, Amaral S, Braun H, Lima-Junior R, Ribeiro R, Cunha F, Teixeira M, Beyaert R, Graham G, Liew F IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice. Mucosal Immunol, 2014-01-15;7(5):1079-93. 2014-01-15 [PMID: 24424522] (Flow Cytometry, Mouse)
• Tung , Hui-Ying, Plunkett , Beverly, Huang , Shau-Ku, Zhou , Yufeng Murine mast cells secrete and respond to interleukin-33. J Interferon Cytokine Res, 2013-09-12;34(3):141-7. 2013-09-12 [PMID: 24028396] (Flow Cytometry, Mouse)
• Mizutani N, Nabe T, Yoshino S Interleukin-33 and alveolar macrophages contribute to the mechanisms underlying the exacerbation of IgE-mediated airway inflammation and remodelling in mice. Immunology, 2013-06-01;139(2):205-18. 2013-06-01 [PMID: 23323935] (Flow Cytometry, Mouse)
• Wills-Karp M, Rani R, Dienger K Trefoil factor 2 rapidly induces interleukin 33 to promote type 2 immunity during allergic asthma and hookworm infection. J. Exp. Med., 2012-02-13;209(3):607-22. 2012-02-13 [PMID: 22329990] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: IL33
• Uniprot:
  • Q8BVZ5()