IL-33 Antibody (396118) [Unconjugated]

Western blot shows lysates of mouse splenocytes. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse IL-33 Monoclonal Antibody (Catalog # MAB3626) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for IL-33 at approximately 18 and 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL-33 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Rat Anti-Mouse IL-33 Monoclonal Antibody (Catalog # MAB3626) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel, Catalog # NL013) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse IL-33 Monoclonal Antibody (Catalog # MAB3626, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Mu
• Applications: WB, CyTOF-ready, Flow, ICC/IF
• Clone: 396118
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-33 Antibody (396118) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IL-33
  Ser109-Ile266
  Accession # Q8BVZ5
• Specificity: Detects mouse IL-33 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL33
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-33 Antibody (396118) [Unconjugated]

• C9orf26
• C9orf26chromosome 9 open reading frame 26 (NF-HEV)
• DKFZp586H0523
• DVS27
• DVS27-related protein
• IL1F11
• IL-1F11
• IL33
• IL-33
• interleukin 33
• Interleukin-1 family member 11
• interleukin-33
• NFHEV
• NF-HEV
• NF-HEVNFEHEV
• Nuclear factor from high endothelial venules
• RP11-575C20.2

Background

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1-3). DVS 27 was identifed as a gene that is upregulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL-1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase-1, generating an N-terminal fragment that is slightly shorter than the C-terminal fragment (3, 4). The N-terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL-1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5-7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL-1R AcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% amino acid (aa) sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.

1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
3. Schmitz, J. et al. (2005) Immunity 23:479.
4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:6930.
7. Dinarello, C.A. (2005) Immunity 23:461.
8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-33 Antibody (MAB3626)(26)

Publications using MAB3626

• Uapinyoying P, Hogarth M, Battacharya S et al. Single-cell transcriptomic analysis of the identity and function of fibro/adipogenic progenitors in healthy and dystrophic muscle iScience 2023-08-18 [PMID: 37599828]
• B Kiaf, K Bode, C Schuster, S Kissler Gata3 is detrimental to regulatory T cell function in autoimmune diabetes bioRxiv : the preprint server for biology, 2023-03-23;0(0):. 2023-03-23 [PMID: 36993342] (Western Blot, Mouse)
• D Jayawarden, S Priyamvada, T Kageyama, Z White, A Kumar, TF Griggs, A Majumder, R Akram, AN Anbazhagan, T Sano, PK Dudeja Loss of SLC26A3 Results in Colonic Mucosal Immune Dysregulation via Epithelial-Immune Cell Crosstalk Cellular and Molecular Gastroenterology and Hepatology, 2022-12-17;0(0):. 2022-12-17 [PMID: 36535508] (In Vivo, Mouse, Transgenic Mouse)
• Deepti Sharma, Shivantika Bisen, Geetika Kaur, Eric C. Van Buren, Gadiparthi N. Rao, Nikhlesh K. Singh IL-33 enhances Jagged1 mediated NOTCH1 intracellular domain (NICD) deubiquitination and pathological angiogenesis in proliferative retinopathy Communications Biology 2022-05-19 [PMID: 35589941]
• S Patial, BW Lewis, T Vo, I Choudhary, K Paudel, Y Mao, D Singamsett, F Brombacher, Y Saini Myeloid-IL4Ralpha is an indispensable link in IL-33-ILCs-IL-13-IL4Ralpha axis of eosinophil recruitment in murine lungs Scientific Reports, 2021-07-29;11(1):15465. 2021-07-29 [PMID: 34326406] (IHC, Mouse)
• S Toyama, CS Moniaga, S Nakae, M Kurosawa, H Ogawa, M Tominaga, K Takamori Regulatory T Cells Exhibit Interleukin-33-Dependent Migratory Behavior during Skin Barrier Disruption International Journal of Molecular Sciences, 2021-07-12;22(14):. 2021-07-12 [PMID: 34299063] (Neutralization, Mouse)
• F Kubo, DM Ariestanti, S Oki, T Fukuzawa, R Demizu, T Sato, RM Sabirin, S Hirose, N Nakamura Loss of the adhesion G-protein coupled receptor ADGRF5 in mice induces airway inflammation and the expression of CCL2 in lung endothelial cells Respir. Res., 2019-01-17;20(1):11. 2019-01-17 [PMID: 30654796] (Western Blot, Mouse)
• Z He, L Chen, GC Furtado, SA Lira Interleukin 33 regulates gene expression in intestinal epithelial cells independently of its nuclear localization Cytokine, 2018-08-23;111(0):146-153. 8/23/2018 [PMID: 30145369]
• Mark Barbour, Rachel Wood, Shehla U. Hridi, Chelsey Wilson, Grant McKay, Trevor J. Bushell et al. The therapeutic effect of anti-CD52 treatment in murine experimental autoimmune encephalomyelitis is associated with altered IL-33 and ST2 expression levels Journal of Neuroimmunology 2018-05-01 [PMID: 29526407]
• Kang H, Yang K, Xiao L et al. Osteoblast Hypoxia-Inducible Factor-1a Pathway Activation Restrains Osteoclastogenesis via the Interleukin-33-MicroRNA-34a-Notch1 Pathway. Front Immunol. 10/31/2017 [PMID: 29085370]
• F Qi, S Bai, D Wang, L Xu, H Hu, S Zeng, R Chai, B Liu Macrophages produce IL-33 by activating MAPK signaling pathway during RSV infection Mol. Immunol., 2017-05-19;87(0):284-292. 2017-05-19 [PMID: 28531812] (Western Blot, Mouse)
• Liu T, Kanaoka Y, Barrett N, Feng C, Garofalo D, Lai J, Buchheit K, Bhattacharya N, Laidlaw T, Katz H, Boyce J Aspirin-Exacerbated Respiratory Disease Involves a Cysteinyl Leukotriene-Driven IL-33-Mediated Mast Cell Activation Pathway. J Immunol, 2015-09-04;195(8):3537-45. 2015-09-04 [PMID: 26342029] (IHC-P, Mouse)
• Wu J, Carlock C, Zhou C, Nakae S, Hicks J, Adams H, Lou Y IL-33 is required for disposal of unnecessary cells during ovarian atresia through regulation of autophagy and macrophage migration. J Immunol, 2015-01-23;194(5):2140-7. 2015-01-23 [PMID: 25617473] (IHC, Mouse)
• Chen , Fei, Wu , Wenhui, Millman , Ariel, Craft , Joshua F, Chen , Eunice, Patel , Nirav, Boucher , Jean L, Urban , Joseph F, Kim , Charles, Gause , William Neutrophils prime a long-lived effector macrophage phenotype that mediates accelerated helminth expulsion. Nat Immunol, 2014-08-31;15(10):938-46. 2014-08-31 [PMID: 25173346] (Flow Cytometry, Mouse)
• Schiering C, Krausgruber T, Chomka A, Frohlich A, Adelmann K, Wohlfert E, Pott J, Griseri T, Bollrath J, Hegazy A, Harrison O, Owens B, Lohning M, Belkaid Y, Fallon P, Powrie F The alarmin IL-33 promotes regulatory T-cell function in the intestine. Nature, 2014-07-16;513(7519):564-8. 2014-07-16 [PMID: 25043027] (Western Blot, Mouse)
• Carlock C, Wu J, Zhou C, Tatum K, Adams H, Tan F, Lou Y Unique temporal and spatial expression patterns of IL-33 in ovaries during ovulation and estrous cycle are associated with ovarian tissue homeostasis. J Immunol, 2014-05-23;193(1):161-9. 2014-05-23 [PMID: 24860190] (Neutralization, Mouse)
• Huang J, Tsai Y, Chang Y, Wu J, Hung J, Lin K, Wong C, Yu A alpha-Galactosylceramide but not phenyl-glycolipids induced NKT cell anergy and IL-33-mediated myeloid-derived suppressor cell accumulation via upregulation of egr2/3. J Immunol, 2014-01-24;192(4):1972-81. 2014-01-24 [PMID: 24465013] (Flow Cytometry, Mouse)
• Guabiraba R, Besnard A, Menezes G, Secher T, Jabir M, Amaral S, Braun H, Lima-Junior R, Ribeiro R, Cunha F, Teixeira M, Beyaert R, Graham G, Liew F IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice. Mucosal Immunol, 2014-01-15;7(5):1079-93. 2014-01-15 [PMID: 24424522] (IHC, Neutralization, Mouse)
• Mizutani N, Nabe T, Yoshino S Interleukin-33 and alveolar macrophages contribute to the mechanisms underlying the exacerbation of IgE-mediated airway inflammation and remodelling in mice. Immunology, 2013-06-01;139(2):205-18. 2013-06-01 [PMID: 23323935] (Neutralization, Mouse)
• Juncadella , Ignacio, Kadl , Alexandr, Sharma , Ashish K, Shim , Yun M, Hochreiter-Hufford , Amelia, Borish , Larry, Ravichandran , Kodi S Apoptotic cell clearance by bronchial epithelial cells critically influences airway inflammation. Nature, 2012-12-12;493(7433):547-51. 2012-12-12 [PMID: 23235830] (Neutralization, Mouse)
• Kaieda S, Wang J, Shnayder R, Fishgal N, Hei H, Lee R, Stevens R, Nigrovic P Interleukin-33 primes mast cells for activation by IgG immune complexes. PLoS ONE, 2012-10-11;7(10):e47252. 10/11/2012 [PMID: 23071771]
• Hsu C, Bryce P Inducible IL-33 expression by mast cells is regulated by a calcium-dependent pathway. J Immunol, 2012-08-24;189(7):3421-9. 2012-08-24 [PMID: 22922818] (ICC, Mouse)
• Jiang HR, Milovanovic M, Allan D, Niedbala W, Besnard AG, Fukada SY, Alves-Filho JC, Togbe D, Goodyear CS, Linington C, Xu D, Lukic ML, Liew FY IL-33 attenuates EAE by suppressing IL-17 and IFN-gamma production and inducing alternatively activated macrophages. Eur. J. Immunol., 2012-06-12;42(7):1804-14. 2012-06-12 [PMID: 22585447] (IHC-Fr, Mouse)
• Yasuoka S, Kawanokuchi J, Parajuli B, Jin S, Doi Y, Noda M, Sonobe Y, Takeuchi H, Mizuno T, Suzumura A Production and functions of IL-33 in the central nervous system. Brain Res., 2011-02-22;1385(0):8-17. 2011-02-22 [PMID: 21349253] (IHC-Fr, Western Blot, Mouse)
• Hsu CL, Neilsen CV, Bryce PJ IL-33 is produced by mast cells and regulates IgE-dependent inflammation. PLoS ONE, 2010-08-03;5(8):e11944. 2010-08-03 [PMID: 20689814] (Flow Cytometry, Mouse)
• Hudson CA, Christophi GP, Gruber RC, Wilmore JR, Lawrence DA, Massa PT Induction of IL-33 expression and activity in central nervous system glia. J. Leukoc. Biol., 2008-06-13;84(3):631-43. 2008-06-13 [PMID: 18552204] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: IL33
• Uniprot:
  • Q8BVZ5()