IL-31 Antibody [Unconjugated]

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IL‑31 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Goat Anti-Human IL‑31 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N, ELISA(Cap), ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

IL-31 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human IL-31
Ser24-Thr164
Accession # Q6EBC2
Specificity
Detects human IL‑31 in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant mouse IL-31 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IL31
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Neutralization 1 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Human IL-31 Antibody (Catalog # AF2824)
ELISA Detection: Human IL-31 Biotinylated Antibody (Catalog # BAF2824)
Standard: Recombinant Human IL-31 (Catalog # 2824-IL)

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-31 Antibody [Unconjugated]

  • IL31
  • IL-31
  • IL-31interleukin-31
  • interleukin 31

Background

Human Interleukin-31 (IL-31) is a 24 kDa, short-chain member of the alpha -helical family of cytokines. The human IL-31 cDNA encodes a 164 amino acid (aa) precursor that contains a 23 aa signal peptide and a 141 aa mature protein (1, 2). The mature region shows four alpha -helices which would be expected to show a typical up‑up‑down‑down topology. Human and mouse IL-31 share 24% aa sequence identity in the mature region (1). IL-31 is mainly associated with activated T cells and preferentially expressed by Th2 rather than Th1 cells. IL-31 signals via a heterodimeric receptor complex composed of a 120 kDa, gp130-related molecule termed IL‑31 RA (also GPL and GLM-R) and the 180 kDa oncostatin M receptor (OSM R beta ) (2‑6). In the complex, IL-31 directly binds to GPL, not OSM R (2, 3). IL-31 signaling has been shown to involve the Jak/STAT pathway, the PI3 kinase/AKT cascade, and the MAP kinase pathway (2‑5). Although multiple isoforms of IL-31 RA are known, only a form that contains the entire length of the cytoplasmic domain is signaling-capable (2, 3). The IL-31 receptor is constitutively expressed by keratinocytes and upregulated by IFN-gamma on monocytes (1). Studies using transgenic mice indicate that IL-31 may contribute to the pruritis (itching) associated with nonatopic dermatitis (1, 7).

  1. Dillon, S.R. et al. (2004) Nat. Immunol. 5:752.
  2. Diveu, C. et al. (2004) Eur. Cytokine Netw. 15:291.
  3. Dreuw, A. et al. (2004) J. Biol. Chem. 279:36112.
  4. Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.
  5. Ghilardi, N. et al. (2002) J. Biol. Chem 277:16831.
  6. Mosley, B. et al. (1996) J. Biol. Chem. 271:32635.
  7. Takaoka, A. et al. (2005) Eur. J. Pharmacol. 516:180.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IL31
Uniprot