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NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox" target="_blank">
2824-IL) was serially diluted and captured by Goat Anti-Human IL‑31 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2824) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑31 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2824) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
2824-IL) was serially diluted and captured by Goat Anti-Human IL‑31 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2824) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑31 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2824) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting ofIL31RA (MIM 609510) and OSMR (MIM 601743) that is constitutively expressed on epithelial cells and keratinocytes. IL31may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma(Dillon et al., 2004 (PubMed 15184896)).(supplied by OMIM)