IL-23R Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-23R Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-23 R
  Gly24-Ile353
  Accession # Q5VWK5
• Specificity: Detects human IL-23 R in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse IL-23 R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL23R
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-23R Antibody [Biotin]

• IBD17
• IL-23 R
• IL-23 receptor
• IL23R
• IL-23R
• interleukin 23 receptor
• interleukin-23 receptor

Background

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Human IL-23 R cDNA encodes a 629 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 330 aa residue extracellular domain, a 23 aa residue transmembrane domain, and a 253 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential STAT-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% aa sequence identity. Based on quantitative real-time PCR, human IL-23 R mRNA is expressed in a human Th1 and Th0 clone as well as several NK cell lines and clones. Low but detectable levels of IL-23 R mRNA is also expressed in EBV-transformed B cells and activated PBMC. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, STAT1, STAT3, STAT4, and STAT5. IL-23 has biological activities that are similar to, but distinct from, IL-12.

1. Oppmann, B. et al. (2000) Immunity 13:715.
2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
3. Parham, C. et al. (2002) J. Immunol. 168:5448.
4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-23R Antibody (BAF1400)(11)

Publications using BAF1400

• J Georgy, Y Arlt, JM Moll, M Ouzin, HT Weitz, L Gremer, D Willbold, J Grötzinger, F Thives-Kur, J Scheller, DM Floss Tryptophan (W) at position 37 of murine IL-12/IL-23 p40 is mandatory for binding to IL-12Rbeta1 and subsequent signal transduction The Journal of Biological Chemistry, 2021-10-09;0(0):101295. 2021-10-09 [PMID: 34637790] (Western Blot, Mouse)
• Dong Hyun Kim, Hee Young Kim, Sunjung Cho, Su-Jin Yoo, Won-Ju Kim, Hye Ran Yeon et al. Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1 beta -dependent response of Th17 cells eLife 1/28/2021 [PMID: 33507149]
• A Esch, A Masiarz, S Mossner, JM Moll, J Grötzinger, J Schröder, J Scheller, DM Floss Deciphering site 3 interactions of IL-12 and IL-23 with their cognate murine and human receptors J. Biol. Chem., 2020-06-09;0(0):. 2020-06-09 [PMID: 32518162] (ICC, Mouse)
• D Liu, S Xing, W Wang, X Huang, H Lin, Y Chen, K Lan, L Chen, F Luo, S Qin, R Liang, C Bai, J Xu, W Liu Prognostic value of serum soluble interleukin-23 receptor and related T-helper 17 cell cytokines in non-small cell lung carcinoma Cancer Sci., 2020-03-04;111(4):1093-1102. 2020-03-04 [PMID: 32020720] (ELISA (detection), Human)
• Moyu Ming, Chaoqian Li, Zhixi Luo, Shengqiu Lv, Qixiang Sun The effect of inhaled inactived Mycobacterium phlei as a treatment for asthma Molecular Medicine Reports 3/1/2017 [PMID: 28035388]
• Yu R, Brazaitis J, Gallagher G The human IL-23 receptor rs11209026 A allele promotes the expression of a soluble IL-23R-encoding mRNA species. J Immunol, 2014-12-31;194(3):1062-8. 2014-12-31 [PMID: 25552541] (ELISA Development (Detection), Human)
• Di Meglio P, Di Cesare A, Laggner U The IL23R R381Q Gene Variant Protects against Immune-Mediated Diseases by Impairing IL-23-Induced Th17 Effector Response in Humans. PLoS ONE, 2011-02-22;6(2):e17160. 2011-02-22 [PMID: 21364948] (Flow Cytometry, Human)
• Yu RY, Gallagher G A naturally occurring, soluble antagonist of human IL-23 inhibits the development and in vitro function of human Th17 cells. J. Immunol., 2010-11-12;185(12):7302-8. 2010-11-12 [PMID: 21076058] (ELISA Development, Human)
• Lee WW, Kang SW, Choi J Regulating human Th17 cells via differential expression of IL-1 receptor. Blood, 2009-11-12;115(3):530-40. 2009-11-12 [PMID: 19965648] (Flow Cytometry, Human)
• Boniface K, Bak-Jensen KS, Li Y, Blumenschein WM, McGeachy MJ, McClanahan TK, McKenzie BS, Kastelein RA, Cua DJ, de Waal Malefyt R Prostaglandin E2 regulates Th17 cell differentiation and function through cyclic AMP and EP2/EP4 receptor signaling. J. Exp. Med., 2009-03-09;206(3):535-48. 2009-03-09 [PMID: 19273625] (Flow Cytometry, Human)
• de Paus RA, van de Wetering D, Van Dissel JT IL-23 and IL-12 responses in activated human T cells retrovirally transduced with IL-23 receptor variants. Mol. Immunol., 2008-09-01;45(15):3889-95. 2008-09-01 [PMID: 18675459] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL23R
• Uniprot:
  • Q5VWK5()