IL-23A/IL-23 P19 Antibody (320244) [Phycoerythrin]

RAW 264.7 mouse monocyte/macrophage cell line untreated (open histogram) or treated with LPS (filled histogram) was stained with Rat Anti-Mouse IL-23 PE-conjugated Monoclonal Antibody (Catalog # IC18871P, filled histogram) or isotype control antibody (Catalog # IC006P, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Mu
• Applications: Flow
• Clone: 320244
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

IL-23A/IL-23 P19 Antibody (320244) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant mouse IL-23 p19 and recombinant mouse IL-12/IL-23 p40
  Leu20-Ala196 (Il-23 p19) & Met23-Ser335 (IL-12/IL-23 p40)
  Accession # Q9EQ14 (Il-23 p19) & P43432 (IL-12/IL-23 p40)
• Specificity: Detects mouse IL-23 in flow cytometry.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL23A
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-23A/IL-23 P19 Antibody (320244) [Phycoerythrin]

• IL-23 p19/IL-12 p40
• IL23
• IL-23
• IL-23A
• IL-23-A
• IL-23p19
• IL-23p19/IL-12p40
• IL23P19P19
• interleukin 23 p19 subunit
• interleukin 23, alpha subunit p19
• interleukin-23 subunit alpha
• Interleukin-23 subunit p19
• JKA3 induced upon T-cell activation
• MGC79388
• SGRF
• SGRFIL-23 subunit alpha

Background

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1-5). The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Mouse p19 cDNA encodes a 196 amino acid residue (aa) precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naïve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent pro-inflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8^- and CD8⁺ subtypes, however only IL-23 can act directly on CD8⁺ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide.

1. Oppmann, B. et al. (2000) Immunity 13:715.
2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
3. Parham, C. et al. (2002) J. Immunol. 168:5699.
4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-23A/IL-23 P19 Antibody (IC18871P)(1)

Publication using IC18871P

• M Nakao, T Miyagaki, M Sugaya, S Sato Exacerbated Imiquimod-Induced Psoriasis-Like Skin Inflammation in IRF5-Deficient Mice Int J Mol Sci, 2020-05-23;21(10):. 2020-05-23 [PMID: 32456211] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: IL23A
• Uniprot:
  • Q9EQ14 (Il-23 p19) & P43432 (IL-12/IL-23 p40)()