IL-22 Antibody (142928) [Phycoerythrin]

PBMC treated with 20ng/mL PMA and 1ug/mL Calcium iono for 2 hours followed by 1ug/mL brefeldin A for 4 hours were stained with Mouse Anti-Human CD4 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB3791F) and either (A) Mouse Anti-Human IL‑22 PE Monoclonal Antibody (Catalog # IC7821P) or (B) isotype control antibody (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Human peripheral blood mononuclear cells (PBMCs) treated with PMA, Ca++Ionomycin, LPS, and Recombinant Human IL-23 (Catalog # 1290-IL) were stained with Mouse Anti-Human IL-22 PE-conjugated Monoclonal Antibody (Catalog # IC7821P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

• Reactivity: Hu
• Applications: Flow
• Clone: 142928
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

IL-22 Antibody (142928) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human IL-22
  Ala34-Ile179
  Accession # Q9GZX6
• Specificity: Detects human IL-22 in direct ELISAs. In direct ELISAs, this antibody shows 100% cross-reactivity with recombinant mouse IL‑22 and recombinant rat IL‑22 and no cross-reactivity with recombinant human IL‑10.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL22
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-22 Antibody (142928) [Phycoerythrin]

• Cytokine Zcyto18
• IL-10-related T-cell-derived inducible factor
• IL22
• IL-22
• IL-D110
• IL-TIF
• ILTIFIL-10-related T-cell-derived-inducible factor
• IL-TIFMGC79382
• interleukin 22
• interleukin-22
• MGC79384
• TIFa
• TIFIL-23
• zcyto18

Background

Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta , share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22 R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.

1. Dumoutier, L. et al. (2000) J. Immunol. 164:1814.
2. Xie, M-H. et al. (2000) J. Biol. Chem. 275:31335.
3. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144.
4. Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-22 Antibody (IC7821P)(38)

Publications using IC7821P

• M Lo Conte, I Cosorich, R Ferrarese, M Antonini C, A Nobili, V Palmieri, L Massimino, LA Lamparelli, W Liang, M Riba, E Devecchi, AM Bolla, E Pedone, M Scavini, E Bosi, A Fasano, F Ungaro, J Diana, N Mancini, M Falcone Alterations of the intestinal mucus layer correlate with dysbiosis and immune dysregulation in human Type 1 Diabetes EBioMedicine, 2023-04-14;91(0):104567. 2023-04-14 [PMID: 37062177] (Flow Cytometry, Human)
• Miqdad O. Dhariwala, Dhuvarakesh Karthikeyan, Kimberly S. Vasquez, Sepideh Farhat, Antonin Weckel, Keyon Taravati et al. Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature Cell Reports Medicine 2020-11-03 [PMID: 33294857]
• Juan Liu, Biao Shang, Jing Bai IL‑22/IL‑22R1 promotes proliferation and collagen synthesis of MRC‑5 cells via the JAK/STAT3 signaling pathway and regulates airway subepithelial fibrosis Experimental and Therapeutic Medicine 2020-06-24 [PMID: 32765690] (Flow Cytometry, Human)
• Juan Liu, Biao Shang, Jing Bai IL‑22/IL‑22R1 promotes proliferation and collagen synthesis of MRC‑5 cells via the JAK/STAT3 signaling pathway and regulates airway subepithelial fibrosis Experimental and Therapeutic Medicine 6/24/2020 [PMID: 32765690]
• Kaat de Jonge, Anna Ebering, Sina Nassiri, Hélène Maby-El Hajjami, Hajer Ouertatani-Sakouhi, Petra Baumgaertner et al. Circulating CD56bright NK cells inversely correlate with survival of melanoma patients Scientific Reports 2019-03-14 [PMID: 30872676]
• R Ahn, D Yan, HW Chang, K Lee, S Bhattarai, ZM Huang, M Nakamura, R Singh, L Afifi, K Taravati, P Munoz-Sand, M Pauli, MD Rosenblum, W Liao RNA-seq and flow-cytometry of conventional, scalp, and palmoplantar psoriasis reveal shared and distinct molecular pathways Sci Rep, 2018-07-27;8(1):11368. 2018-07-27 [PMID: 30054515] (Flow Cytometry, Human)
• MS Gresnigt, C Cunha, M Jaeger, SM Gonçalves, RKS Malireddi, A Ammerdorff, R Lubbers, M Oosting, O Rasid, G Jouvion, C Fitting, DJ Jong, JF Lacerda, A Campos, WJG Melchers, K Lagrou, J Maertens, TD Kanneganti, A Carvalho, O Ibrahim-Gr, FL van de Vee Genetic deficiency of NOD2 confers resistance to invasive aspergillosis Nat Commun, 2018-07-06;9(1):2636. 2018-07-06 [PMID: 29980664] (Flow Cytometry, Human)
• N Li, V van Unen, T Höllt, A Thompson, J van Bergen, N Pezzotti, E Eisemann, A Vilanova, SM Chuva de S, BPF Lelieveldt, F Koning Mass cytometry reveals innate lymphoid cell differentiation pathways in the human fetal intestine J. Exp. Med., 2018-03-06;0(0):. 2018-03-06 [PMID: 29511064] (Bioassay, Human)
• AS Chitre, MG Kattah, YY Rosli, M Pao, M Deswal, SG Deeks, PW Hunt, M Abdel-Mohs, LJ Montaner, CC Kim, A Ma, M Somsouk, JM McCune A20 upregulation during treated HIV disease is associated with intestinal epithelial cell recovery and function PLoS Pathog., 2018-03-05;14(3):e1006806. 2018-03-05 [PMID: 29505600] (Flow Cytometry, Mouse)
• Thomas J. Scriba, Adam Penn-Nicholson, Smitha Shankar, Tom Hraha, Ethan G. Thompson, David Sterling et al. Sequential inflammatory processes define human progression from M. tuberculosis infection to tuberculosis disease PLOS Pathogens 2017-11-16 [PMID: 29145483]
• I Cosorich, G Dalla-Cost, C Sorini, R Ferrarese, MJ Messina, J Dolpady, E Radice, A Mariani, PA Testoni, F Canducci, G Comi, V Martinelli, M Falcone High frequency of intestinal TH17 cells correlates with microbiota alterations and disease activity in multiple sclerosis Sci Adv, 2017-07-12;3(7):e1700492. 2017-07-12 [PMID: 28706993] (Flow Cytometry, Human)
• KM Cordoro, M Hitraya-Lo, K Taravati, PM Sandoval, E Kim, J Sugarman, ML Pauli, W Liao, MD Rosenblum Skin-infiltrating, interleukin-22-producing T cells differentiate pediatric psoriasis from adult psoriasis J. Am. Acad. Dermatol., 2017-06-16;0(0):. 2017-06-16 [PMID: 28624119] (Flow Cytometry, Human)
• M Forkel, L Berglin, E Kekäläinen, A Carlsson, E Svedin, J Michaëlsso, M Nagasawa, JS Erjefält, M Mori, M Flodström-, A Bergquist, HG Ljunggren, M Westgren, U Lindforss, D Friberg, C Jorns, E Ellis, NK Björkström, J Mjösberg Composition and functionality of the intrahepatic innate lymphoid cell-compartment in human non-fibrotic and fibrotic livers Eur. J. Immunol., 2017-07-11;0(0):. 2017-07-11 [PMID: 28613415] (Flow Cytometry, Human)
• MH Nada, H Wang, G Workalemah, Y Tanaka, CT Morita Enhancing adoptive cancer immunotherapy with V?2V?2 T cells through pulse zoledronate stimulation J Immunother Cancer, 2017-02-21;5(0):9. 2017-02-21 [PMID: 28239463] (Flow Cytometry, Human)
• W Zhong, L Zhao, T Liu, Z Jiang IL-22-producing CD4+T cells in the treatment response of rheumatoid arthritis to combination therapy with methotrexate and leflunomide Sci Rep, 2017-01-24;7(0):41143. 2017-01-24 [PMID: 28117352] (Flow Cytometry, Human)
• J Suurmond, KL Habets, AL Dorjée, TW Huizinga, RE Toes Expansion of Th17 Cells by Human Mast Cells Is Driven by Inflammasome-Independent IL-1? J. Immunol, 2016-10-31;197(11):4473-4481. 2016-10-31 [PMID: 27799306] (Flow Cytometry, Human)
• Ling Y, Cypowyj S, Aytekin C, Galicchio M, Camcioglu Y, Nepesov S, Ikinciogullari A, Dogu F, Belkadi A, Levy R, Migaud M, Boisson B, Bolze A, Itan Y, Goudin N, Cottineau J, Picard C, Abel L, Bustamante J, Casanova J, Puel A Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis. J Exp Med, 2015-04-27;212(5):619-31. 2015-04-27 [PMID: 25918342] (Neutralization, Human)
• Zhou M, Zou R, Gan H, Liang Z, Li F, Lin T, Luo Y, Cai X, He F, Shen E The effect of aging on the frequency, phenotype and cytokine production of human blood CD4 + CXCR5 + T follicular helper cells: comparison of aged and young subjects. Immun Ageing, 2014-08-23;11(0):12. 2014-08-23 [PMID: 25177353] (Flow Cytometry, Human)
• Shen H, Wang Y, Chen C, Frencher J, Huang D, Yang E, Ryan-Payseur B, Chen Z Th17-related cytokines contribute to recall-like expansion/effector function of HMBPP-specific Vgamma2Vdelta2 T cells after Mycobacterium tuberculosis infection or vaccination. Eur J Immunol, 2015-02-01;45(2):442-51. 2015-02-01 [PMID: 25141829] (Flow Cytometry, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque))
• Longman R, Diehl G, Victorio D, Huh J, Galan C, Miraldi E, Swaminath A, Bonneau R, Scherl E, Littman D CX(3)CR1(+) mononuclear phagocytes support colitis-associated innate lymphoid cell production of IL-22. J Exp Med, 2014-07-14;211(8):1571-83. 2014-07-14 [PMID: 25024136] (Flow Cytometry, Human)
• Teunissen M, Munneke J, Bernink J, Spuls P, Res P, Te Velde A, Cheuk S, Brouwer M, Menting S, Eidsmo L, Spits H, Hazenberg M, Mjosberg J Composition of innate lymphoid cell subsets in the human skin: enrichment of NCR(+) ILC3 in lesional skin and blood of psoriasis patients. J Invest Dermatol, 2014-03-21;134(9):2351-60. 2014-03-21 [PMID: 24658504] (Flow Cytometry, Human)
• Guo H, Peng D, Yang X, Wang Y, Xu B, Ni J, Meng W, Jiang Y A higher frequency of circulating IL-22(+)CD4(+) T cells in Chinese patients with newly diagnosed Hashimoto's thyroiditis. PLoS ONE, 2014-01-03;9(1):e84545. 2014-01-03 [PMID: 24404171] (Flow Cytometry, Human)
• Gironi , Maira, Saresella , Marina, Rovaris , Marco, Vaghi , Matilde, Nemni , Raffaell, Clerici , Mario, Grossi , Enzo A novel data mining system points out hidden relationships between immunological markers in multiple sclerosis. Immun Ageing, 2013-01-10;10(1):1. 2013-01-10 [PMID: 23305498] (Flow Cytometry, Human)
• Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barre B, Audran M, Bouvard B, Saint-Andre J, Jeannin P IL-26 Is Overexpressed in Rheumatoid Arthritis and Induces Proinflammatory Cytokine Production and Th17 Cell Generation. PLoS Biol, 2012-09-25;10(9):e1001395. 2012-09-25 [PMID: 23055831] (Flow Cytometry, Human)
• Duhen T, Duhen R, Lanzavecchia A Functionally distinct subsets of human FOXP3+ Treg cells that phenotypically mirror effector Th cells. Blood, 2012-03-21;119(19):4430-40. 2012-03-21 [PMID: 22438251] (Flow Cytometry, Human)
• Miyagaki T, Sugaya M, Suga H, Kamata M, Ohmatsu H, Fujita H, Asano Y, Tada Y, Kadono T, Sato S IL-22, but not IL-17, dominant environment in cutaneous T-cell lymphoma. Clin. Cancer Res., 2011-11-02;17(24):7529-38. 2011-11-02 [PMID: 22048239] (Flow Cytometry, Human)
• Luyan Liu, Satoshi Okada, Xiao-Fei Kong, Alexandra Y. Kreins, Sophie Cypowyj, Avinash Abhyankar et al. Gain-of-function human STAT1 mutations impair IL-17 immunity and underlie chronic mucocutaneous candidiasis Journal of Experimental Medicine 2011-08-01 [PMID: 21727188]
• Qiao D, Yang BY, Li L, Ma JJ, Zhang XL, Lao SH, Wu CY ESAT-6- and CFP-10-specific Th1, Th22 and Th17 cells in tuberculous pleurisy may contribute to the local immune response against Mycobacterium tuberculosis infection. Scand. J. Immunol., 2011-04-01;73(4):330-7. 2011-04-01 [PMID: 21223348] (Flow Cytometry, Human)
• Liu Y, Yang B, Ma J, Wang H, Huang F, Zhang J, Chen H, Wu C Interleukin-21 induces the differentiation of human Tc22 cells via phosphorylation of signal transducers and activators of transcription. Immunology, 2011-01-07;132(4):540-8. 2011-01-07 [PMID: 21214545] (Flow Cytometry, Human)
• Res PC, Piskin G, de Boer OJ, van der Loos CM, Teeling P, Bos JD, Teunissen MB Overrepresentation of IL-17A and IL-22 producing CD8 T cells in lesional skin suggests their involvement in the pathogenesis of psoriasis. PLoS ONE, 2010-11-24;5(11):e14108. 2010-11-24 [PMID: 21124836] (Flow Cytometry, Human)
• Ramirez JM, Brembilla NC, Sorg O, Chicheportiche R, Matthes T, Dayer JM, Saurat JH, Roosnek E, Chizzolini C Activation of the aryl hydrocarbon receptor reveals distinct requirements for IL-22 and IL-17 production by human T helper cells. Eur. J. Immunol., 2010-09-01;40(9):2450-9. 2010-09-01 [PMID: 20706985] (Flow Cytometry, Human)
• Kisand K, Boe Wolff AS, Podkrajsek KT, Tserel L, Link M, Kisand KV, Ersvaer E, Perheentupa J, Erichsen MM, Bratanic N, Meloni A, Cetani F, Perniola R, Ergun-Longmire B, Maclaren N, Krohn KJ, Pura M, Schalke B, Strobel P, Leite MI, Battelino T, Husebye ES, Peterson P, Willcox N, Meager A Chronic mucocutaneous candidiasis in APECED or thymoma patients correlates with autoimmunity to Th17-associated cytokines. J. Exp. Med., 2010-02-01;207(2):299-308. 2010-02-01 [PMID: 20123959] (Flow Cytometry, Human)
• Carbone T, Nasorri F, Pennino D CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses. J. Immunol., 2009-12-11;184(2):1102-10. 2009-12-11 [PMID: 20008290] (Flow Cytometry, Human)
• Duhen T, Geiger R, Jarrossay D, Lanzavecchia A, Sallusto F Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells. Nat. Immunol., 2009-07-05;10(8):857-63. 2009-07-05 [PMID: 19578369] (Flow Cytometry, Human)
• Hughes T, Becknell B, McClory S, Briercheck E, Freud AG, Zhang X, Mao H, Nuovo G, Yu J, Caligiuri MA Stage 3 immature human natural killer cells found in secondary lymphoid tissue constitutively and selectively express the TH 17 cytokine interleukin-22. Blood, 2009-02-24;113(17):4008-10. 2009-02-24 [PMID: 19244159] (Flow Cytometry, Human)
• Eyerich K, Pennino D, Scarponi C, Foerster S, Nasorri F, Behrendt H, Ring J, Traidl-Hoffmann C, Albanesi C, Cavani A IL-17 in atopic eczema: linking allergen-specific adaptive and microbial-triggered innate immune response. J. Allergy Clin. Immunol., 2008-12-03;123(1):59-66.e4. 2008-12-03 [PMID: 19056110] (Flow Cytometry, Human)
• Nistala K, Moncrieffe H, Newton KR, Varsani H, Hunter P, Wedderburn LR Interleukin-17-producing T cells are enriched in the joints of children with arthritis, but have a reciprocal relationship to regulatory T cell numbers. Arthritis Rheum., 2008-03-01;58(3):875-87. 2008-03-01 [PMID: 18311821] (Flow Cytometry, Human)
• Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells. Nat. Immunol., 2007-08-05;8(9):942-9. 2007-08-05 [PMID: 17676045] (Flow Cytometry, Human)

Reviews for IL-22 Antibody (IC7821P) (8)

Average Rating: 4

(Based on 8 reviews)

We have 8 reviews tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 02/03/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 22621183
• Species: Human

reviewed by: Verified Customer Flow Human 02/03/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 22621183
• Species: Human

reviewed by: Verified Customer Flow Human 02/03/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 21525997
• Species: Human

reviewed by: Verified Customer Flow Human 02/03/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 21525997
• Species: Human

reviewed by: Verified Customer Flow Human 02/03/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 21206487
• Species: Human

reviewed by: Verified Customer Flow Human 02/03/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 21206487
• Species: Human

reviewed by: Verified Customer Flow Human 01/28/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 22070501
• Species: Human

reviewed by: Verified Customer Flow Human 01/28/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 22070501
• Species: Human

Bioinformatics

• Gene Symbol: IL22
• Uniprot:
  • Q9GZX6()