Recombinant Mouse IL-21 Protein

Product Discontinued

Recombinant Mouse IL-21 Protein Summary

• Description: Recombinant bioactive protein containing 130 amino acids for Mouse IL-21
  
  

  Source:E. coli
  Amino Acid Sequence:(Q9ES17)

• Details of Functionality: The bioactivity is determined by a dose-dependent proliferation of lymphocytes co-stimulated with anti-CD3E and is typically 2-50 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL21
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: <0.01 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 15.095 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 20 mM NaHCO3, pH 8.5
• Concentration: Lyoph
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute with sterile H2O to a concentration of 0.1 mg/ml, which can be further diluted into other aqueous solutions.

Alternate Names for Recombinant Mouse IL-21 Protein

• CVID11
• IL21
• IL-21
• IL-21Za11interleukin-21
• interleukin 21
• interleukin-21 isoform
• Za11

Background

Mouse Interleukin-21 (m-IL-21), expressed by activated CD4(+) T cells, is the newest member of the common gamma-chain family of cytokines, which also includes IL-2, IL-4, IL-7, IL-9, and IL-13. Its private receptor, IL-21R, is expressed on T, B, and NK cells. Within the B cell lineage, IL-21 regulates IgG1 production and cooperates with IL-4 for the production of multiple Ab classes in vivo. IL-21R has been shown to activate the Janus kinase/signal transducers and activators of transcription signaling pathways upon ligand binding. Initial studies have demonstrated that IL-21 has pleiotropic effects on the proliferation, the differentiation, and the effector functions of B, T, natural killer, and dendritic cells. Recombinant mouse IL-21, produced in E.coli, is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a total molecular mass of 15,095 Daltons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL21