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Product Details

Reactivity HuSpecies Glossary
Applications ELISA
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate)

Order Details

IL-21 ELISA Kit Summary

Standard Curve Range
78 - 5,000 pg/mL
20.0 pg/mL
Kit Type

Packaging, Storage & Formulations

Storage of components varies. See protocol for specific instructions.
Concentration is not relevant for this product. Please see the protocols for proper use of this product.


Application Notes
The human IL-21 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-21

Kit Components

  1. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-21
  2. Biotin-Conjugate anti-human IL-21 monoclonal antibody
  3. Streptavidin-HRP
  4. Human IL-21 Standard lyophilized, 10 ng/mL upon reconstitution
  5. Sample Diluent
  6. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  7. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  8. Substrate Solution (tetramethyl-benzidine)
  9. Stop Solution (1M Phosphoric acid)
  10. Adhesive Films

Reactivity Notes



Colored dyes for optional use with this kit are available and will be provided free of charge upon request. Please refer to catalog number NBP2-41404.

Alternate Names for IL-21 ELISA Kit

  • IL21
  • IL-21Za11interleukin-21
  • interleukin 21
  • interleukin-21 isoform


Interleukin-21 is a member of the type-I cytokine family. Structurally, IL-21 shows homology to IL-2, IL-4, and IL-15 proteins. IL-21 shares the common gamma-chain with these three cytokines, but, in addition, binds to a unique IL-21R alpha chain, and activates the JAK/STAT pathway. IL-21 has pleiotropic actions, from augmenting the proliferation of T cells and driving the differentiation of B cells into memory cells and terminally differentiated plasma cells to augmenting the activity of natural killer cells. IL-21 is mainly produced by activated T-cells, but targets a broad range of lymphoid and myeloid cells of the immune system and therefore is able to regulate innate and acquired immune responses. IL-21 has pathologic function in immune-inflammatory diseases. Data suggest the existence of a positive autocrine loop that could help to amplify and stabilize IL-21-driven, T cell-mediated responses. Moreover, it has antitumour activity and may have a role in the development of autoimmunity. IL-21 may have therapeutic potentials as an antitumor agent in the clinic.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Blogs on IL-21.

Perforin Antibodies Reveal Links to Apoptosis and Immune Response
Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol IL21

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