Human IL-21 ELISA Kit (Colorimetric)

Images

 
ELISA: IL-21 ELISA Kit [NBP1-84816] - Standard curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate)
Standard Curve Range
78 - 5,000 pg/mL
Sensitivity
20.0 pg/mL

Order Details

Human IL-21 ELISA Kit (Colorimetric) Summary

Specificity
The assay detects both natural and recombinant human IL-21. The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into serum. There was no crossreactivity detected.
Standard Curve Range
78 - 5,000 pg/mL
Sensitivity
20.0 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
7.7%
Intra-Assay
5.8%
Spike Recovery
The mean recovery for sera was 71%, for plasma (EDTA) 42%, for plasma (citrate) 58% and for cell culture supernatant 95%.
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL21

Applications/Dilutions

Application Notes
This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  2. Streptavidin-HRP
  3. Substrate Solution (tetramethyl-benzidine)
  4. Stop Solution (1M Phosphoric acid)
  5. Sample Diluent
  6. Human IL-21 Standard lyophilized, 10 ng/mL upon reconstitution
  7. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  8. Biotin-Conjugate anti-human IL-21 monoclonal antibody
  9. Adhesive Films
  10. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-21

Alternate Names for Human IL-21 ELISA Kit (Colorimetric)

  • IL21
  • IL-21
  • IL-21Za11interleukin-21
  • interleukin 21
  • interleukin-21 isoform

Background

The human IL-21 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-21. Interleukin-21 is a member of the type-I cytokine family. Structurally, IL-21 shows homology to IL-2, IL-4, and IL-15 proteins. IL-21 shares the common gamma-chain with these three cytokines, but, in addition, binds to a unique IL-21R alpha chain, and activates the JAK/STAT pathway. IL-21 has pleiotropic actions, from augmenting the proliferation of T cells and driving the differentiation of B cells into memory cells and terminally differentiated plasma cells to augmenting the activity of natural killer cells. IL-21 is mainly produced by activated T-cells, but targets a broad range of lymphoid and myeloid cells of the immune system and therefore is able to regulate innate and acquired immune responses. IL-21 has pathologic function in immune-inflammatory diseases. Data suggest the existence of a positive autocrine loop that could help to amplify and stabilize IL-21-driven, T cell-mediated responses. Moreover, it has antitumour activity and may have a role in the development of autoimmunity. IL-21 may have therapeutic potentials as an antitumor agent in the clinic.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
596-MR
Species: Mu
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
NBP2-22471
Species: Hu, Mu, Rt, Mk, Pm
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
MAB15041
Species: Hu
Applications: WB, IHC, CyTOF-ready, ICFlow
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NB100-39002
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-31369
Species: Hu
Applications: WB, IHC, IHC-P

Publications for IL-21 ELISA Kit (NBP1-84816) (0)

There are no publications for IL-21 ELISA Kit (NBP1-84816).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IL-21 ELISA Kit (NBP1-84816) (0)

There are no reviews for IL-21 ELISA Kit (NBP1-84816). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-21 ELISA Kit (NBP1-84816) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IL-21 Products

Bioinformatics Tool for IL-21 ELISA Kit (NBP1-84816)

Discover related pathways, diseases and genes to IL-21 ELISA Kit (NBP1-84816). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-21 ELISA Kit (NBP1-84816)

Discover more about diseases related to IL-21 ELISA Kit (NBP1-84816).
 

Pathways for IL-21 ELISA Kit (NBP1-84816)

View related products by pathway.

PTMs for IL-21 ELISA Kit (NBP1-84816)

Learn more about PTMs related to IL-21 ELISA Kit (NBP1-84816).
 

Research Areas for IL-21 ELISA Kit (NBP1-84816)

Find related products by research area.

Blogs on IL-21.

Perforin Antibodies Reveal Links to Apoptosis and Immune Response
Perforin, also known as the pore-forming protein, pfp, is a 70 kD cytolytic protein expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. It is one of the major effector molecules used by CTL and NK cel...  Read full blog post.

Customers Who Bought This Also Bought

IL-21
594-ML-010/CF
IL-4 [Unconjugated]
204-IL-010/CF

Contact Information

Product PDFs

Review this Product

Be the first to review our Human IL-21 ELISA Kit (Colorimetric) and receive a gift card or discount.

Bioinformatics

Gene Symbol IL21
Entrez