IL-2 R beta Antibody (5H4) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: IL-2 R beta Antibody (5H4) - Chimeric [NBP3-12040] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 5H4 (NBP3-12040) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12040, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

• Reactivity: Mu
• Applications: Flow, ICC/IF
• Clone: 5H4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

IL-2 R beta Antibody (5H4) - Chimeric - Azide and BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was raised by immunising Lewis rats with transfected rat myeloma cell line YB2/0, expressing truncated murine IL-2R beta.
• Specificity: This antibody is specific for the murine interleukin-2 receptor beta chain.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IL2RB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IL-2 R beta Antibody (5H4) - Chimeric - Azide and BSA Free

• CD122 antigen
• CD122
• high affinity IL-2 receptor beta subunit
• High affinity IL-2 receptor subunit beta
• IL-15 R beta
• IL-2 R beta
• IL-2 receptor subunit beta
• IL2R beta
• IL-2R subunit beta
• IL2RB
• IL-2Rb
• interleukin 15 receptor, beta
• interleukin 2 receptor, beta
• interleukin-2 receptor subunit beta
• P70-75
• p75

Background

The Interleukin 2 Receptor alpha and beta chains, together with the common gamma chain, constitute the high affinity IL2 receptor present on activated T and B cells, thymocyte subset, pre B cells and T regulatory cells. Homodimeric alpha chains result in low affinity receptor, while homodimeric beta chains produce a medium affinity receptor. Normally an integral membrane protein, soluble IL2 Receptor alpha has been isolated and determined to result from extracellular proteolysis. Alternately spliced IL2 Receptor alpha mRNAs have been isolated, but the significance of each is presently unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL2RB