IL-2 Antibody [Unconjugated]

Recombinant Canine IL‑2 (Cys147Ser) (Catalog # 1815-CL) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL‑2 (Cys147Ser) (10 ng/mL) is neutralized (green line) by increasing concentrations of Canine IL‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1815). The ND50 is typically 0.25-1.5 µg/mL.

• Reactivity: Ca
• Applications: B/N, ELISA(Cap)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant canine IL‑2 (R&D Systems, Catalog # 1815-CL)
  Ala21-Thr155 with a Cys147Ser substitution
  Accession # Q29416
• Specificity: Detects canine IL-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human IL‑2, recombinant mouse IL-2, recombinant rat IL-2, recombinant feline IL-2, recombinant cotton rat IL-2, recombinant bovine IL‑2, recombinant equine IL‑2, and recombinant porcine IL-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL2
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.25-1.5 ug/mL
• Application Notes: ELISA Capture: Canine IL-2 Antibody (Catalog # AF1815)
  ELISA Detection: Canine IL-2 Biotinylated Antibody (Catalog # BAF1815)
  Standard: Recombinant Canine IL-2 (Cys147Ser) (Catalog # 1815-CL)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-2 Antibody [Unconjugated]

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens. Since then, it has been shown that IL-2 can also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages, and oligodendrocytes. The biological activity of IL-2 is mediated by the binding to cell surface receptor complexes composed of three subunits designated as alpha , beta , and gamma subunits. IL-2 binds the  alpha subunit with low affinity. The functional high affinity IL-2 receptor is a heterotrimeric complex of the alpha , beta , and gamma subunits. IL-2 binds with intermediate affinity to the complex containing the  beta and  gamma subunits, which is also capable of transducing IL-2 signals. In T cells, the beta and gamma subunits are shared with the IL-15 receptor complex. The gamma subunit of the IL-2 receptor complex has also been shown to be a subunit of the receptor complexes of IL-4, IL-7, and IL-9. At the amino acid sequence level, canine IL-2 shares 90%, 86%, 85%, 76%, and 75% sequence similarities to feline, human, equine, mouse, and bovine IL-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL2
• Uniprot:
  • Q29416()