IL-18R alpha/IL-1 R5 Antibody [Unconjugated]


In the presence of Recombinant Mouse IL‑12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Mouse IL‑18/IL‑1F4 stimulates IFN‑ gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as more
B6 splenocytes were stained with Rat Anti-Mouse CD3 PE‑conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Goat Anti-Mouse IL‑18 R alpha /IL‑1 R5 Antigen more

Product Details

Reactivity MuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready

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IL-18R alpha/IL-1 R5 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R alpha /IL-1 R5
Accession # Q61098
Detects mouse IL‑18 R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human IL‑18 R is observed.
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 0.2-0.8 ug/mL
  • Western Blot 0.1 ug/mL
Read Publications using
AF856 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18R alpha/IL-1 R5 Antibody [Unconjugated]

  • CD218a
  • IL-1 R5
  • IL-1 Rrp
  • IL-18 R alpha
  • IL-18 R1
  • IL18R alpha
  • IL18R1
  • IL-18Ra


Interleukin-18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immuno-regulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL18 in vitro, coexpression of IL-18 R alpha and IL18 R beta is required for high affinity binding and IL-18 responsiveness.

Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa residues. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-18R alpha/IL-1 R5 Antibody (AF856)(10)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: Flow Cytometry, IHC, IHC-Fr, In Vivo, Neutralization.

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Showing Publications 1 - 10 of 10.
Publications using AF856 Applications Species
JE Howard, JNP Smith, G Fredman, KC MacNamara IL-18R-mediated HSC quiescence and MLKL-dependent cell death limit hematopoiesis during infection-induced shock Stem Cell Reports, 2021;0(0):. 2021 [PMID: 34798063] (In Vivo, Mouse) In Vivo Mouse
T Hayakawa, M Hata, S Kuwahara-O, H Yagi, H Okamura Postnatal changes of interleukin-18 receptor immunoreactivity in neurons of the retrosplenial cortex in wild-type and interleukin-18 knock out mice Okajimas Folia Anat Jpn, 2017;94(3):93-99. 2017 [PMID: 29681594] (IHC, Mouse) IHC Mouse
S Kuwahara-O, S Maeda, K Kobayashi, Y Minato, K Tanaka, K Yamanishi, M Hata, W Li, T Hayakawa, K Noguchi, H Okamura, H Yagi Interleukin-18 and its receptor are expressed in gonadotropin-releasing hormone neurons of mouse and rat forebrain Neurosci. Lett., 2017;0(0):. 2017 [PMID: 28373090]
Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS). J. Biol. Chem., 2009;0(0):. 2009 [PMID: 19592492] (Neutralization, Mouse) Neutralization Mouse
Wiener Z, Pocza P, Racz M, Nagy G, Tolgyesi G, Molnar V, Jaeger J, Buzas E, Gorbe E, Papp Z, Rigo J, Falus A IL-18 induces a marked gene expression profile change and increased Ccl1 (I-309) production in mouse mucosal mast cell homologs. Int. Immunol., 2008;20(12):1565-73. 2008 [PMID: 18940934] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Andoh T, Kishi H, Motoki K, Nakanishi K, Kuraishi Y, Muraguchi A Protective effect of IL-18 on kainate- and IL-1beta-induced cerebellar ataxia in mice. J. Immunol., 2008;180(4):2322-8. 2008 [PMID: 18250441] (IHC-Fr, Mouse) IHC-Fr Mouse
Chandrasekar B, Patel DN, Mummidi S, Kim JW, Clark RA, Valente AJ Interleukin-18 suppresses adiponectin expression in 3T3-L1 adipocytes via a novel signal transduction pathway involving ERK1/2-dependent NFATc4 phosphorylation. J. Biol. Chem., 2007;283(7):4200-9. 2007 [PMID: 18086672] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Chaudhry UI, Kingham TP, Plitas G, Katz SC, Raab JR, DeMatteo RP Combined stimulation with interleukin-18 and CpG induces murine natural killer dendritic cells to produce IFN-gamma and inhibit tumor growth. Cancer Res., 2006;66(21):10497-504. 2006 [PMID: 17079471] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Wang X, Hagberg H, Mallard C, Zhu C, Hedtjarn M, Tiger CF, Eriksson K, Rosen A, Jacobsson B Disruption of interleukin-18, but not interleukin-1, increases vulnerability to preterm delivery and fetal mortality after intrauterine inflammation. Am. J. Pathol., 2006;169(3):967-76. 2006 [PMID: 16936270] (IHC-Fr, Mouse) IHC-Fr Mouse
Gould MP, Greene JA, Bhoj V, DeVecchio JL, Heinzel FP Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis. J. Immunol., 2004;172(3):1754-62. 2004 [PMID: 14734758] (Neutralization, Mouse) Neutralization Mouse

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Gene Symbol Il18rap