IL-18R alpha/IL-1 R5 Antibody [Unconjugated]

IL‑18 Ra/IL‑1 R5 was detected in immersion fixed frozen sections of human skin using Goat Anti-Human IL‑18 Ra/IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of keratinocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Recombinant Human IL‑18/IL‑1F4 stimulates IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line, in a dose-dependent manner (orange line), as measured by the Human IFN‑ gamma Quantikine ELISA Kit (Catalog # DIF50). IFN‑ gamma secretion elicited by IL‑18/IL‑1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑18 R alpha /IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840). The ND50 is typically 0.1-0.4 µg/mL.

• Reactivity: Hu
• Applications: WB, Flow, IHC, B/N, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-18R alpha/IL-1 R5 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5
  Glu20-Arg329
  Accession # Q13478
• Specificity: Detects human IL‑18 R alpha /IL-1 R5 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse IL‑18 R alpha is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL18R1
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.1-0.4 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18R alpha/IL-1 R5 Antibody [Unconjugated]

• CD218a
• CD218a;CDw218a;IL18R1;IL18RA;IL-18Ralpha;IL-18R-alpha;IL18Ralpha2;IL1RRP;IL-1Rrp;Interleukin-18 receptor 1
• IL-1 R5
• IL-1 Rrp
• IL-18 R alpha
• IL-18 R1
• IL18R alpha
• IL18R1
• IL-18Ra

Background

Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immuno-regulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL‑18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa residues. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate, and placenta.

1. Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
2. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
3. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-18R alpha/IL-1 R5 Antibody (AF840)(11)

Publications using AF840

• Higashi, Y;Dashek, R;Delafontaine, P;Rector, RS;Chandrasekar, B; EF24, a Curcumin Analog, Reverses Interleukin-18-Induced miR-30a or miR-342-Dependent TRAF3IP2 Expression, RECK Suppression, and the Proinflammatory Phenotype of Human Aortic Smooth Muscle Cells Cells 2024-10-10 [PMID: 39451191] (Neutralization, Human)
• Chauffier, J;Berger de Gallardo, H;Chevalier, MF;Kante, A;Lambert, M;Cabrol, X;Aldersons, E;Mouly, S;Champion, K;Amador-Borrero, B;Burlacu, R;Bigot, W;Adle-Biassete, H;Kaci, R;Selvanadin, A;Cohen-Solal, M;Coudert, A;Caillat-Zucman, S;Sène, D;Comarmond, C; Role of mucosal-associated invariant T cells dynamics in pathogenesis of Sjögren syndrome Scientific reports 2024-07-27 [PMID: 39060324] (Immunohistochemistry, Human)
• Zaman, SU;Pagare, PP;Huang, B;Rilee, G;Ma, Z;Zhang, Y;Li, J; Novel PROTAC probes targeting FOSL1 degradation to eliminate head and neck squamous cell carcinoma cancer stem cells Bioorganic chemistry 2024-07-09 [PMID: 39002513] (Bioassay, Human)
• Joana Dias, Julia Hengst, Tiphaine Parrot, Edwin Leeansyah, Sebastian Lunemann, David F.G. Malone et al. Chronic hepatitis delta virus infection leads to functional impairment and severe loss of MAIT cells Journal of Hepatology 8/1/2019 [PMID: 31100314]
• Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma Sci Rep, 2016-05-26;6(0):26757. 2016-05-26 [PMID: 27225603] (Western Blot, Human)
• A Gibbs, E Leeansyah, A Introini, D Paquin-Pro, K Hasselrot, E Andersson, K Broliden, JK Sandberg, A Tjernlund MAIT cells reside in the female genital mucosa and are biased towards IL-17 and IL-22 production in response to bacterial stimulation Mucosal Immunol, 2016-04-06;0(0):. 2016-04-06 [PMID: 27049062] (IHC, Human)
• Valente AJ, Yoshida T, Izadpanah R et al. Interleukin-18 enhances IL-18R/Nox1 binding, and mediates TRAF3IP2-dependent smooth muscle cell migration. Inhibition by simvastatin. Cell Signal. 6/1/2013 [PMID: 23541442]
• Dong J, Chang H, Ivascu C, Qian Y, Rezai S, Okhrimenko A, Cosmi L, Maggi L, Eckhardt F, Wu P, Sieper J, Alexander T, Annunziato F, Gossen M, Li J, Radbruch A, Thiel A Loss of methylation at the IFNG promoter and CNS-1 is associated with the development of functional IFN-gamma memory in human CD4(+) T lymphocytes. Eur J Immunol, 2013-03-01;43(3):793-804. 2013-03-01 [PMID: 23255246] (Flow Cytometry, Human)
• Dusseaux M, Martin E, Serriari N, Peguillet I, Premel V, Louis D, Milder M, Le Bourhis L, Soudais C, Treiner E, Lantz O Human MAIT cells are xenobiotic-resistant, tissue-targeted, CD161hi IL-17-secreting T cells. Blood, 2010-11-17;117(4):1250-9. 2010-11-17 [PMID: 21084709] (ICC, IHC-Fr, Human)
• Sallmon H, Hoene V, Weber SC, Dame C Differentiation of human SH-SY5Y neuroblastoma cells by all-trans retinoic acid activates the interleukin-18 system. J. Interferon Cytokine Res., 2010-02-01;30(2):55-8. 2010-02-01 [PMID: 20028206] (Western Blot, Human)
• Felderhoff-Mueser U, Sifringer M, Polley O, Dzietko M, Leineweber B, Mahler L, Baier M, Bittigau P, Obladen M, Ikonomidou C, Buhrer C Caspase-1-processed interleukins in hyperoxia-induced cell death in the developing brain. Ann. Neurol., 2005-01-01;57(1):50-9. 2005-01-01 [PMID: 15622543] (IHC-P, Western Blot, Rat)

Bioinformatics

• Gene Symbol: IL18R1
• Uniprot:
  • Q13478()