IL-18R alpha/IL-1 R5 Antibody (112624)

In the presence of Recombinant Mouse IL‑12 (0.1 ng/mL, Catalog # 419-ML), Recombinant Mouse IL‑18/IL‑1F4 stimulates IFN‑ gamma secretion in activated mouse T cells in a dose-dependent manner (orange line), as measured by the Mouse IFN‑ gamma Quantikine ELISA Kit (Catalog # MIF00). Under these conditions, IFN‑ gamma secretion elicited by Recombinant Mouse IL‑18/IL‑1F4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑18 R alpha /IL‑1 R5 Monoclonal Antibody (Catalog # MAB12161). The ND50 is typically 0.2-0.8 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clone: 112624
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-18R alpha/IL-1 R5 Antibody (112624) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R alpha
  Ser19-Gly326
  Accession # Q61098
• Specificity: Detects mouse IL-18 R in direct ELISAs. In direct ELISAs, this antibody does not cross-react with recombinant mouse (rm) IL-1 RI, rmIL‑1 RII, rhIL‑1 R3, recombinant human (rh) IL-1 R4, rhIL-1 R5, recombinant rat IL-1 R6, rmIL-1 R7, rhIL-1 R8, rhIL-1 R9, or rmSIGIRR.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Il18rap
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.2-0.8 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18R alpha/IL-1 R5 Antibody (112624)

• CD218a
• CD218a;CDw218a;IL18R1;IL18RA;IL-18Ralpha;IL-18R-alpha;IL18Ralpha2;IL1RRP;IL-1Rrp;Interleukin-18 receptor 1
• IL-1 R5
• IL-1 Rrp
• IL-18 R alpha
• IL-18 R1
• IL18R alpha
• IL18R1
• IL-18Ra

Background

IL-18 R, also known as IL-18 R alpha, is a member of the IL-1 receptor family and has been designated IL-1 R5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-18R alpha/IL-1 R5 Antibody (MAB12161)(6)

Publications using MAB12161

• Rakebrandt, N;Yassini, N;Kolz, A;Schorer, M;Lambert, K;Goljat, E;Estrada Brull, A;Rauld, C;Balazs, Z;Krauthammer, M;Carballido, JM;Peters, A;Joller, N; Innate acting memory Th1 cells modulate heterologous diseases Proceedings of the National Academy of Sciences of the United States of America 2024-06-11 [PMID: 38838013] (Neutralization, Mouse)
• XIAOCUI LI, CUIPING ZHANG, WEI CHEN, BIN PAN, FANYUN KONG, KUIYANG ZHENG et al. Protective effect of neutralizing anti-IL-18 alpha monoclonal antibody on a mouse model of acute graft-versus-host disease Oncology Reports 10/1/2015 [PMID: 26252430]
• Wang J, Sun C, Gerdes N, Liu C, Liao M, Liu J, Shi M, He A, Zhou Y, Sukhova G, Chen H, Cheng X, Kuzuya M, Murohara T, Zhang J, Cheng X, Jiang M, Shull G, Rogers S, Yang C, Ke Q, Jelen S, Bindels R, Ellison D, Jarolim P, Libby P, Shi G Interleukin 18 function in atherosclerosis is mediated by the interleukin 18 receptor and the Na-Cl co-transporter. Nat Med, 2015-06-22;21(7):820-6. 2015-06-22 [PMID: 26099046] (Immunoprecipitation, Mouse)
• Ghose P, Ali AQ, Fang R, Forbes D, Ballard B, Ismail N The interaction between IL-18 and IL-18 receptor limits the magnitude of protective immunity and enhances pathogenic responses following infection with intracellular bacteria. J. Immunol., 2011-06-29;187(3):1333-46. 2011-06-29 [PMID: 21715688] (Neutralization, Mouse)
• Li W, Kashiwamura S, Ueda H, Sekiyama A, Okamura H Protection of CD8+ T cells from activation-induced cell death by IL-18. J. Leukoc. Biol., 2007-03-30;82(1):142-51. 2007-03-30 [PMID: 17400610] (Neutralization, Mouse)
• Kang MJ, Homer RJ, Gallo A, Lee CG, Crothers KA, Cho SJ, Rochester C, Cain H, Chupp G, Yoon HJ, Elias JA IL-18 is induced and IL-18 receptor alpha plays a critical role in the pathogenesis of cigarette smoke-induced pulmonary emphysema and inflammation. J. Immunol., 2007-02-01;178(3):1948-59. 2007-02-01 [PMID: 17237446] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: Il18rap
• Uniprot:
  • Q61098()