IL-18 R beta/IL-1 R7/ACPL Antibody (132029) [Phycoerythrin]

KG-1 human acute myelogenous leukemia cell line was stained with Mouse Anti-Human IL-18 R beta /IL-1 R7 PE-conjugated Mono-clonal Antibody (Catalog # FAB118P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 132029
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

IL-18 R beta/IL-1 R7/ACPL Antibody (132029) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL‑18 R beta /IL‑1 R7
  Met1-Arg356
  Accession # O95256
• Specificity: Detects human IL‑18 R beta /IL‑1 R7 in direct ELISAs and Western blots. In direct ELISAs, 15% cross-reactivity with recombinant human (rh) IL‑1 RII is observed and 2% cross-reactivity with rhIL-1 R9 is observed. Also in direct ELISAs, no cross-reactivity with rhIL-1 RI, rhIL‑1 RacP/IL‑1 R3, rhIL-1 R4, rhIL-18 R alpha /IL-1 R5, rhIL-1 R6, rhIL-1 R8, or recombinant mouse (rm) IL-18 R beta /IL-1 R7 is observed. In Western blots, approximately 100% cross-reactivity with rmIL-18 R beta is observed and no cross-reactivity with rhIL-18 R alpha /IL-1 R5, rhIL‑1 RacP/IL‑1 R3, rhIL-1 RI, rhIL-1 RII, or rhIL-1 Rrp2/IL-1 R6 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL18RAP
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18 R beta/IL-1 R7/ACPL Antibody (132029) [Phycoerythrin]

• Accessory protein-like
• AcPL
• CD218 antigen-like family member B
• CD218b antigen
• CD218b
• CDw218b
• IL-1 R7
• IL-18 R beta
• IL-18 receptor accessory protein
• IL-18 receptor beta
• IL18R beta
• IL-18RAcP
• IL18RAP
• IL18RB
• IL-18Rb
• IL-18Rbeta
• IL-18R-beta
• IL-1R accessory protein-like
• IL1R7
• IL-1R7
• IL-1R-7
• IL-1RAcPL
• interleukin 18 receptor accessory protein
• Interleukin-1 receptor 7
• interleukin-18 receptor accessory protein
• Interleukin-18 receptor accessory protein-like
• Interleukin-18 receptor beta
• MGC120589
• MGC120590

Background

IL-18, originally described as an interferon-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines that has multiple immunoregulatory functions. It has potent IFN-gamma inducing activities and plays a key role in the activation of T helper type 1 (Th1) responses. The functional IL-18 receptor complex consists of two components, the IL‑18 R alpha (IL-1 R5) and IL-18 R beta (also termed IL-1 R7 and AcPL) subunits. Both subunits are members of the IL-1 receptor superfamily. Although IL‑18 R alpha by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R beta cDNA encodes a 599 amino acid (aa) residue precursor type I membrane protein with a 14 aa signal peptide, a 342 aa extracellular region containing three immunoglobulin-like domains, a single transmembrane domain and a 222 aa cytoplasmic domain. Human and mouse IL-18 R beta share 65% aa sequence identity. The expression of IL-18 R beta parallels that of IL-18 R alpha and is detected in numerous tissues including lung, spleen, leukocytes and colon.

1. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
2. Okamura, H. et al. (2000) in Cytokine Reference, Vol. 2:1605, Academic Press.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-18 R beta/IL-1 R7/ACPL Antibody (FAB118P)(7)

Publications using FAB118P

• SJ Choi, JY Koh, MS Rha, IH Seo, H Lee, S Jeong, SH Park, EC Shin KIR+CD8+ and NKG2A+CD8+ T�cells are distinct innate-like populations in humans Cell Reports, 2023-03-09;42(3):112236. 2023-03-09 [PMID: 36897779] (Flow Cytometry, Human)
• Avishai Shemesh, Harry Pickering, Kole T. Roybal, Lewis L. Lanier Differential IL-12 signaling induces human natural killer cell activating receptor-mediated ligand-specific expansion Journal of Experimental Medicine 2022-08-01 [PMID: 35758909]
• Spallanzani R, Torres N, Avila D, Ziblat A, Iraolagoitia X, Rossi L, Domaica C, Fuertes M, Rabinovich G, Zwirner N Regulatory Dendritic Cells Restrain NK Cell IFN-gamma Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors. J Immunol, 2015-07-31;195(5):2141-8. 2015-07-31 [PMID: 26232426] (Flow Cytometry, Human)
• Florent Guerville, Sophie Daburon, Romain Marlin, Lydia Lartigue, Severine Loizon, Vincent Pitard et al. TCR-dependent sensitization of human gamma δ T cells to non-myeloid IL-18 in cytomegalovirus and tumor stress surveillance OncoImmunology 2015-05-04 [PMID: 26155394]
• Uzan B, Poglio S, Gerby B, Wu C, Gross J, Armstrong F, Calvo J, Cahu X, Deswarte C, Dumont F, Passaro D, Besnard-Guerin C, Leblanc T, Baruchel A, Landman-Parker J, Ballerini P, Baud V, Ghysdael J, Baleydier F, Porteu F, Pflumio F Interleukin-18 produced by bone marrow-derived stromal cells supports T-cell acute leukaemia progression. EMBO Mol Med, 2014-04-06;6(6):821-34. 2014-04-06 [PMID: 24778454] (Flow Cytometry, Human)
• Increased expression of Interleukin-18 receptor in blood cells of subjects with Mild Cognitive Impairment and Alzheimer's disease. Cytokine, 2012-11-30;61(2):360-3. 2012-11-30 [PMID: 23201485] (Flow Cytometry, Human)
• De Jager W, Vastert SJ, Beekman JM, Wulffraat NM, Kuis W, Coffer PJ, Prakken BJ Defective phosphorylation of interleukin-18 receptor beta causes impaired natural killer cell function in systemic-onset juvenile idiopathic arthritis. Arthritis Rheum., 2009-09-01;60(9):2782-93. 2009-09-01 [PMID: 19714583] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL18RAP
• Uniprot:
  • O95256()