IL-18/IL-1F4 Antibody (159-12B) [Biotin]

• Reactivity: Hu
• Applications: ELISA(Det)
• Clone: 159-12B
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Biotin

IL-18/IL-1F4 Antibody (159-12B) [Biotin] Summary

• Immunogen: Human IL-18/IL-1F4
• Specificity: This antibody reacts with human IL-18 on ELISA.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL18
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: Standard: Recombinant Human IL-18/IL-1F4 (Catalog number B001-5)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: This antibody solution is stable for one year from the date of purchase when stored at 4°C.
• Buffer: 25 μg IgG in 50 μL volume of PBS containing 1% BSA and 0.09% sodium azide
• Preservative: 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18/IL-1F4 Antibody (159-12B) [Biotin]

• Iboctadekin
• IFN-gamma-inducing factor
• IGIF
• IGIFIL-1 gamma
• IL18
• IL-18
• IL-18MGC12320
• IL-1F4
• IL1F4iboctadekin
• IL-1g
• Interferon gamma-inducing factor
• interleukin 18 (interferon-gamma-inducing factor)
• Interleukin-1 gamma
• interleukin-18

Background

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma . Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.

1. McCurnin, D., et al., Pediatrics 121, 945-956 (2008)
2. Haveman, L. M., et al., Int. Immunol. 18, 1521-1529 (2006)
3. Shigehara, K., et al., J. Immunol. 166, 642-649 (2001)
4. Dao, T., et al. Cell Immunol. 173, 230-235 (1996)
5. Micallef, M., et al. Eur. J. Immunol. 26, 1647-1651 (1996)
6. Ushio, S., et al. J. Immunol. 156, 4274-4279 (1996)
7. Okamura, H., et al. Nature 378, 88-91 (1995)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-18/IL-1F4 Antibody (D045-6)(11)

Publications using D045-6

• Menachem, A;Alteber, Z;Cojocaru, G;Fridman Kfir, T;Blat, D;Leiderman, O;Galperin, M;Sever, L;Cohen, N;Cohen, K;Granit, RZ;Vols, S;Frenkel, M;Soffer, L;Meyer, K;Menachem, K;Galon Tilleman, H;Morein, D;Borukhov, I;Toporik, A;Perpinial Shahor, M;Tatirovsky, E;Mizrachi, A;Levy-Barda, A;Sadot, E;Strenov, Y;Eitan, R;Jakobson-Setton, A;Yanichkin, N;Ferre, P;Ophir, E; Unleashing Natural IL18 Activity Using an Anti-IL18BP Blocker Induces Potent Immune Stimulation and Antitumor Effects Cancer immunology research 2024-04-01 [PMID: 38592331] (ELISA Detection, Human)
• Mikhail A. Gavrilin, Evan R. Prather, Alex D. Vompe, Christian C. McAndrew, Mark D. Wewers cAbl Kinase Regulates Inflammasome Activation and Pyroptosis via ASC Phosphorylation The Journal of Immunology 2021-03-15 [PMID: 33568399]
• SY Nuñez, A Ziblat, F Secchiari, NI Torres, JM Sierra, XL Raffo Irao, RE Araya, CI Domaica, MB Fuertes, NW Zwirner Human M2 Macrophages Limit NK Cell Effector Functions through Secretion of TGF-? and Engagement of CD85j J. Immunol., 2017-12-27;0(0):. 2017-12-27 [PMID: 29282306] (ELISA Development (Detection), Human)
• E Briend, GJ Ferguson, M Mori, G Damera, K Stephenson, NA Karp, S Sethi, CK Ward, MA Sleeman, JS Erjefält, DK Finch IL-18 associated with lung lymphoid aggregates drives IFN? production in severe COPD Respir. Res., 2017-08-22;18(1):159. 2017-08-22 [PMID: 28830544] (ELISA Development (Detection), Human)
• LM Scott, AH Bryant, A Rees, B Down, RH Jones, CA Thornton Production and regulation of interleukin-1 family cytokines at the materno-fetal interface Cytokine, 2017-07-13;0(0):. 2017-07-13 [PMID: 28712670] (ELISA Development (Detection), Human)
• Spallanzani R, Torres N, Avila D, Ziblat A, Iraolagoitia X, Rossi L, Domaica C, Fuertes M, Rabinovich G, Zwirner N Regulatory Dendritic Cells Restrain NK Cell IFN-gamma Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors. J Immunol, 2015-07-31;195(5):2141-8. 2015-07-31 [PMID: 26232426] (ELISA Development (Detection), Human)
• Jitprasertwong P, Jaedicke K, Nile C, Preshaw P, Taylor J Leptin enhances the secretion of interleukin (IL)-18, but not IL-1beta, from human monocytes via activation of caspase-1. Cytokine, 2013-11-23;65(2):222-30. 2013-11-23 [PMID: 24275551] (ELISA Development (Capture), Human)
• Hu H, Kwun J, Aizenstein BD, Knechtle SJ Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation, 2009-06-27;87(12):1814-20. 2009-06-27 [PMID: 19543058] (Antibody Array Development, Human)
• Madsen EL, Bruun JM, Skogstrand K, Hougaard DM, Christiansen T, Richelsen B Long-term weight loss decreases the nontraditional cardiovascular risk factors interleukin-18 and matrix metalloproteinase-9 in obese subjects. Metab. Clin. Exp., 2009-07-01;58(7):946-53. 2009-07-01 [PMID: 19409578] (Luminex Development, Human)
• Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA Microarray Development, Human)
• Pfleger C, Schloot N, ter Veld F Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J. Immunol. Methods, 2007-10-22;329(1):214-8. 2007-10-22 [PMID: 17964594] (Luminex Development, Human)

Bioinformatics

• Gene Symbol: IL18