IL-18/IL-1F4 Antibody (159-12B) [Unconjugated]

• Reactivity: Hu
• Applications: IP
• Clone: 159-12B
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IL-18/IL-1F4 Antibody (159-12B) [Unconjugated] Summary

• Immunogen: Human IL-18/IL-1F4
• Specificity: This antibody reacts with human IL-18 on Immunoprecipitation.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL18
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation

Packaging, Storage & Formulations

• Storage: This antibody solution is stable for one year from the date of purchase when stored at -20°C.
• Buffer: 100 μg IgG in 100 μL PBS containing 50% glycerol, pH 7.2. Contains no preservatives.
• Preservative: No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-18/IL-1F4 Antibody (159-12B) [Unconjugated]

• Iboctadekin
• IFN-gamma-inducing factor
• IGIF
• IGIFIL-1 gamma
• IL18
• IL-18
• IL-18MGC12320
• IL-1F4
• IL1F4iboctadekin
• IL-1g
• Interferon gamma-inducing factor
• interleukin 18 (interferon-gamma-inducing factor)
• Interleukin-1 gamma
• interleukin-18

Background

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma . Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.

1. Nussbaumer, O., et al., Blood 118, 2743-2751 (2011)
2. Tu, A., et al., J. Exp. Med. 205, 233-244 (2008)
3. Wu, C., J. Immunol. 170, 5571-5577 (2003)
4. Sugawara, S., J. Immunol. 167, 6568-6575 (2001)
5. Dao, T., et al., Cell Immunol. 173, 230-235 (1996)
6. Micallef, M., et al., Eur. J. Immunol. 26, 1647-1651 (1996)
7. Ushio, S., et al., J. Immunol. 156, 4274-4279 (1996)
8. Okamura, H., et al., Nature 378, 88-91 (1995)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-18/IL-1F4 Antibody (D045-3)(1)

Publication using D045-3

• Madsen EL, Bruun JM, Skogstrand K, Hougaard DM, Christiansen T, Richelsen B Long-term weight loss decreases the nontraditional cardiovascular risk factors interleukin-18 and matrix metalloproteinase-9 in obese subjects. Metab. Clin. Exp., 2009-07-01;58(7):946-53. 2009-07-01 [PMID: 19409578] (Luminex Development, Human)

Bioinformatics

• Gene Symbol: IL18