IL-17RA/IL-17R Antibody (133621)

• Reactivity: Hu
• Applications: WB, ELISA(Cap)
• Clone: 133621
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-17RA/IL-17R Antibody (133621) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL‑17 RA/IL‑17 R
  Leu33-Trp320
  Accession # Q96F46
• Specificity: Detects human IL‑17 RA/IL‑17 R in ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL17RA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Capture: Human IL-17 RA/IL-17 R Antibody (Catalog # MAB1771)
  ELISA Detection: Human IL-17 RA/IL-17 R Biotinylated Antibody (Catalog # BAF177)
  Standard: Recombinant Human IL-17 RA/IL-17 R Fc Chimera (Catalog # 177-IR)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17RA/IL-17R Antibody (133621)

• CD217 antigen
• CD217
• Cdw217
• CDw217interleukin 17 receptor
• hIL-17R
• IL-17 R
• IL-17 RA
• IL-17 receptor A
• IL17RA
• IL-17RA
• IL-17RAMGC10262
• IL17Rinterleukin-17 receptor A
• interleukin 17 receptor A

Background

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor A (IL-17 RA), also known as IL-17 receptor (IL-17 R). IL-17 RA shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 RA mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 RA is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 RA shares 72% aa sequence identity with mouse and rat IL-17 RA. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.

1. Yao, Z. et al. (1997) Cytokine 9:794.
2. Schwander, R. et al. (2000) J. Exp. Med. 191:1233.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17RA/IL-17R Antibody (MAB1771)(2)

Publications using MAB1771

• B Jin, Y Zhang, HD Miller, L He, D Ge, AR Wang, Z You Defect of IL17 Signaling, but Not Centrinone, Inhibits the Development of Psoriasis and Skin Papilloma in Mouse Models Biomedicines, 2022-08-15;10(8):. 2022-08-15 [PMID: 36009523] (IHC, Human)
• Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities. J. Immunol., 2008-09-15;181(6):4299-310. 2008-09-15 [PMID: 18768888] (Neutralization, Human)

Bioinformatics

• Gene Symbol: IL17RA
• Uniprot:
  • Q96F46()