IL-17E/IL-25 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-17E/IL-25 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse IL‑17E/IL-25 (R&D Systems, Catalog # 1399-IL)
  Val17-Ala169
  Accession # NP_542767
• Specificity: Detects mouse IL‑17E/IL-25 in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity recombinant human IL‑17E, recombinant mouse (rm) IL-17, rmIL-17B, rmIL-17C, rmIL-17D, and rmIL-17F is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL25
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse IL-17E/IL-25 Antibody (Catalog # MAB13991)
  ELISA Detection: Mouse IL-17E/IL-25 Biotinylated Antibody (Catalog # BAF1399)
  Standard: Recombinant Mouse IL-17E/IL-25 (Catalog # 1399-IL)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17E/IL-25 Antibody [Biotin]

• IL17E
• IL-17E
• IL25
• IL-25
• interleukin 25
• Interleukin-17E
• interleukin-25

Background

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).

Mouse IL-17E cDNA encodes a 169 amino acid residues (aa) precursor protein with a putative 16 aa signal peptide (5). Mature mouse IL-17E shares 76% and 91% amino sequence (aa) identity with mature human and rat IL-17E, respectively. Mouse IL-17E also shares from 24% to 32% sequence identity with the other mouse IL‑17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland spinal cord and trachea. IL-17E binds and activates IL-17 B Receptor (IL-17B R) (alternatively known as IL-17 Rh1, IL-17E R, and EVI27) (3, 4), which is expressed in kidney and liver, and at lower levels in brain, testis and other endocrine tissues. The expression of IL-17B R is up regulated under inflammatory conditions. Ligation of IL-17E to IL-17 RB induces activation of nuclear factor kappa-B and stimulates the production of the proinflamatory cytokine IL-8 (3). IL-17 has also been found to promote the expression of the prototypical Th2 genes (4, 5).

1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
3. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
4. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
5. Pan, G. et al. (2001) J. Immunol. 167:6569.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17E/IL-25 Antibody (BAF1399)(4)

Publications using BAF1399

• S Li, X Ding, H Zhang, Y Ding, Q Tan IL-25 improves diabetic wound healing through stimulating M2 macrophage polarization and fibroblast activation International immunopharmacology, 2022-02-08;106(0):108605. 2022-02-08 [PMID: 35149293] (Western Blot, Mouse)
• HK Min, JY Won, BM Kim, KA Lee, SJ Lee, SH Lee, HR Kim, KW Kim Interleukin (IL)-25 suppresses IL-22-induced osteoclastogenesis in rheumatoid arthritis via STAT3 and p38 MAPK/I&kappaB&alpha pathway Arthritis Research & Therapy, 2020-09-23;22(1):222. 2020-09-23 [PMID: 32972460] (Human)
• T Yoshizaki, S Itoh, S Yamaguchi, T Numata, A Nambu, N Kimura, H Suto, K Okumura, K Sudo, A Yamaguchi, S Nakae IL-25 exacerbates autoimmune aortitis in IL-1 receptor antagonist-deficient mice Sci Rep, 2019-11-19;9(1):17067. 2019-11-19 [PMID: 31745167] (IHC, Mouse)
• Tamachi T, Maezawa Y, Ikeda K, Kagami S, Hatano M, Seto Y, Suto A, Suzuki K, Watanabe N, Saito Y, Tokuhisa T, Iwamoto I, Nakajima H IL-25 enhances allergic airway inflammation by amplifying a TH2 cell-dependent pathway in mice. J. Allergy Clin. Immunol., 2006-06-21;118(3):606-14. 2006-06-21 [PMID: 16950278] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: IL25
• Uniprot:
  • NP_542767()