Recombinant Mouse IL-17/IL-17A Protein

Bioactivity: Recombinant Mouse IL-17/IL-17A Protein [NBP3-18184] - Mouse IL17/IL-17A Induced the Secretion of IL-6 by NIH-3T3 cells

SDS-Page: Recombinant Mouse IL-17/IL-17A Protein [NBP3-18184] - 1 ug run under (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel, stained with Coomassie Blue. Recombinant Mouse IL-17/IL-17A Protein is a homodimer with a predicted total molecular weight of 30.2 kDa.

Product Discontinued

Recombinant Mouse IL-17/IL-17A Protein Summary

• Description: For prolonged storage, dilute to working aliquots.
  Expiration Date:
  12 months from date of receipt when stored at -20C to-80C as supplied.
  1 month when stored at 4C after reconstituting as directed.
  3 months when stored at -20C to -80C after reconstituting as directed.
• Specificity: IL-17/IL-17A is a dimer, 15.1/30.2kDa (134/268 aa)
• Preparation Method: Protein is derived from genetically modified E. coli.
• Details of Functionality: Production of IL-6 by mouse 3T3 cells, >2.0 x 10^5 units/mg (ED50 acceptance criteria are <5 ng/mL).
• Protein/Peptide Type: Recombinant Protein
• Gene: IL17A
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Lot Specific Information:
  Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 micron filtered solution, 0.1% Trifluoroacetic Acid (TFA)
• Preservative: No Preservative
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Sterile water at 0.1 mg/mL

Alternate Names for Recombinant Mouse IL-17/IL-17A Protein

• CTLA8
• CTLA-8
• CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
• Cytotoxic T-lymphocyte-associated antigen 8
• IL17
• IL-17
• IL17A
• IL-17A
• IL-17Acytotoxic T-lymphocyte-associated protein 8
• IL-17CTLA-8
• IL17interleukin-17A
• interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• interleukin 17A

Background

Interleukin 17 (IL-17) is a pro-inflammatory cytokine produced by TCRalpha/beta+ CD4 CD8 thymocytes, as well as by activated CD4+ and CD4+ CD45RO+ memory T cells (1). IL-17 stimulates the expression of TNFalpha, IL-1 beta, IL-6, and IL-8 (2). In addition, IL-17 is involved in the induction of inducible nitric oxide synthase and cyclooxygenase 2 in chondrocytes (3). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for IL-17/IL-17A Recombinant Protein (NBP3-18184). (Showing 1 - 2 of 2 FAQ).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
   • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Bioinformatics

• Gene Symbol: IL17A