IL-15R alpha Antibody (JM7A4) - BSA Free

Flow Cytometry: IL-15 R alpha Antibody (JM7A4) [NBP1-43238] - Staining of adherent human PBMCs stimulated with LPS and IFN gamma for 24 hours with 0.25 ug of Mouse IgG2b K Isotype Control Purified (NBP1-43317) (open histogram) or 0.25 ug of Anti-Human IL-15 Receptor Purified (filled histogram) followed by Anti-Mouse IgG Biotin and Streptavidin PE. Cells in the large scatter population were used for analysis.

• Reactivity: Hu
• Applications: WB, Flow, IP
• Clone: JM7A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

IL-15R alpha Antibody (JM7A4) - BSA Free Summary

• Description: Novus Biologicals Mouse IL-15R alpha Antibody (JM7A4) - BSA Free (NBP1-43238) is a monoclonal antibody validated for use in WB, Flow and IP. Anti-IL-15R alpha Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Clone JM7A4 was used by HLDA to establish CD designation.
• Immunogen: The immunogen for this antibody was IL15Ra.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL15RA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry <0.5 mg/10^6 cells in 100 ul
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: This JM7A4 antibody has been tested by flow cytometric analysis of LPS and IFN-γ-stimulated PBMCs. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for IL-15R alpha Antibody (JM7A4) - BSA Free

• CD215 antigen
• CD215
• IL15 R alpha
• IL-15 R alpha
• IL-15 receptor subunit alpha
• IL-15R alpha
• IL15RA
• IL-15Ra
• IL-15R-alpha
• interleukin 15 receptor, alpha
• interleukin-15 receptor subunit alpha
• MGC104179

Background

The JM7A4 monoclonal antibody reacts with the IL-15 receptor alpha chain (IL-15Ralpha). IL-15 and IL-2 are cytokines with overlapping, but distinct, biologic effects. Their receptors share 2 subunits, the IL2R-beta and -gamma chains, which are essential for signal transduction. The IL-15R-alpha receptor is structurally related to IL-2R-alpha. However, the IL-15Ralpha alone binds IL-15 with a 1,000-fold higher affinity than that seen with IL-2R-alpha and IL-2. In the human, 3 differentially spliced human IL15R-alpha variants that are all capable of high affinity binding of IL-15 have been isolated. The cytoplasmic domain of IL15R-alpha, like that of IL2R-alpha, is dispensable for mitogenic signaling, suggesting that the primary role of the alpha chains is to confer high affinity binding. At high concentrations, IL-15, like IL-2, is able to signal through a complex of IL2R-beta and IL2R-gamma in the absence of the alpha subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-15R alpha Antibody (NBP1-43238)(4)

Publications using NBP1-43238

• Vamosi G, Bodnar A, Vereb G, Jenei A, Goldman CK, Langowski J, Toth K, Matyus L, Szollosi J, Waldmann TA, Damjanovich S. IL-2 and IL-15 receptor alpha-subunits are coexpressed in a supramolecular receptor cluster in lipid rafts of T cells. Proc Natl Acad Sci U S A;101(30):11082-7. 2004-07-27 [PMID: 15263076]
• Dubois S, Mariner J, Waldmann TA, Tagaya Y. IL-15Ralpha recycles and presents IL-15 In trans to neighboring cells. Immunity. 2002-11-01 [PMID: 12433361] (FLOW, WB)
• Anderson, D et al. Functional characterization of the human interleukin-15 receptor alpha chain and close linkage of IL15RA and IL2RA genes. J. Biol. Chem. 270: 29862-29869. [PMID: 8530383]
• Giri, J et al. Identification and cloning of a novel IL-15 binding protein that is structurally related to the alpha of the IL-2 receptor. EMBO J. 14: 3654-3663. [PMID: 7641685]

Bioinformatics

• Gene Symbol: IL15RA
• Entrez:
  • 3601(Human)
• Uniprot:
  • Q13261()