IL-13 Antibody - BSA Free

Western Blot: IL-13 Antibody [NBP1-42776] - Analysis of recombinant swine IL-13 at 13.2kDa (arrow) raised in yeast. Multiple bands are expected of the glycosylated protein. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with , Inc. Anti-swine IL-13. After washing, membrane was probed with Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody.

Western blot using protein-A purified anti-swine IL-13 antibody shows detection of recombinant swine IL-13 at 13.2kDa raised in yeast. Multiple bands are expected of the glycosylated protein. Protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1:10), and probed with , Inc. Anti-swine IL-13. After washing, membrane was probed with Dylight (TM) 649 Conjugated Anti-Rabbit IgG (H&L) (Donkey) Antibody ().

• Reactivity: Po, Bv, Sh
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: LYOPH

IL-13 Antibody - BSA Free Summary

• Description: This product was Protein-A purified from monospecific antiserum by chromatography
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This protein-A purified IL-13 Antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant protein raised in yeast, corresponding to mature swine IL-13 protein. (Uniprot: B3GDZ5)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL13
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:11000
  • Western Blot 1.5-2 ug/ml
• Application Notes: This product has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13.2 kDa in size corresponding to swine IL-13 protein by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

A BLAST analysis was used to suggest reactivity with IL-13 from swine sources based on 100% homology with the immunizing sequence. The following homologies may indicate chances of cross-reactivity when amino acid similarity and not just exact sequence is accounted: 89-90% homology to IL-13 from sheep, dolphin, beluga, and bovine, 83-88% to human and certain monkey IL-13, 80-82% to dusky titi, macaque, baboon, mangabey, dog, llama, camel, and galago, 75-78% to horse, and 67-72% to rat, mouse, and gerbil. Cross-reactivity with IL-13 from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for IL-13 Antibody - BSA Free

• ALRHMGC116789
• BHR1interleukin-13
• IL13
• IL-13
• IL-13MGC116788
• interleukin 13
• MGC116786
• NC30
• P600

Background

Interleukin 13 (IL-13), of the IL-4 superfamily, is a cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Ra) and at least one IL-13-specific binding chain. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, STAT6. In humans, IL-13 can induce immunoglobulin E (IgE) secretion from activated B cells. In mice, deletion of IL-13 does not markedly affect either Th2 cell development or antigen-specific IgE responses induced by potent allergens. Deletion of IL-4 abrogates these responses. IL-13 acts as a molecular bridge linking allergic inflammatory cells to the non-immune cells in contact with them, thus altering physiological function. Although IL-13 is associated primarily with the induction of airway disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, it also induces airway matrix metalloproteinases as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL13
• Uniprot:
  • B3GDZ5(Porcine)