IL-13 Antibodies

413-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-13 Monoclonal Antibody (Catalog # MAB413). The ND₅₀ is typically 0.3-0.8 µg/mL." class="big_lightbox"> 413-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-13 Monoclonal Antibody (Catalog # MAB413). The ND₅₀ is typically 0.3-0.8 µg/mL." alt="Recombinant Mouse IL-13 (Catalog # 413-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-13 Monoclonal Antibody (Catalog # MAB413). The ND₅₀ is typically 0.3-0.8 µg/mL." class="big_thumb" />

413-ML) was serially diluted and captured by Rat Anti-Mouse IL‑13 Monoclonal Antibody (Catalog # MAB413) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 413-ML) was serially diluted and captured by Rat Anti-Mouse IL‑13 Monoclonal Antibody (Catalog # MAB413) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

413-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA). The ND₅₀ is typically 0.05-0.15 µg/mL." class="big_lightbox"> 413-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA). The ND₅₀ is typically 0.05-0.15 µg/mL." alt="Recombinant Mouse IL-13 (413-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA). The ND₅₀ is typically 0.05-0.15 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

413-ML) was serially diluted and captured by Rat Anti-Mouse IL‑13 Monoclonal Antibody (Catalog # MAB413) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 413-ML) was serially diluted and captured by Rat Anti-Mouse IL‑13 Monoclonal Antibody (Catalog # MAB413) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-413-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

204-IL) and 10 µg/mL Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for 3 days were stained with Mouse Anti-Human IL-13 Monoclonal Antibody (Catalog # MAB213) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P). Quadrant markers were set based on control antibody staining (Catalog # MAB002)." class="big_lightbox"> 204-IL) and 10 µg/mL Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for 3 days were stained with Mouse Anti-Human IL-13 Monoclonal Antibody (Catalog # MAB213) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P). Quadrant markers were set based on control antibody staining (Catalog # MAB002)." alt="Human peripheral blood mononuclear cells (PBMCs) treated with 5 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 10 µg/mL Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for 3 days were stained with Mouse Anti-Human IL-13 Monoclonal Antibody (Catalog # MAB213) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P). Quadrant markers were set based on control antibody staining (Catalog # MAB002)." class="big_thumb" />

213-ILB) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-13 (10 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-Human IL-13 Monoclonal Antibody (Catalog # MAB213). The ND₅₀ is typically 0.15-0.75 µg/mL." class="big_lightbox"> 213-ILB) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-13 (10 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-Human IL-13 Monoclonal Antibody (Catalog # MAB213). The ND₅₀ is typically 0.15-0.75 µg/mL." class="big_thumb" />

213-ILB) was serially diluted and captured by Mouse Anti-Human IL‑13 Monoclonal Antibody (Catalog # MAB213) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-213-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 213-ILB) was serially diluted and captured by Mouse Anti-Human IL‑13 Monoclonal Antibody (Catalog # MAB213) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-213-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

213-ILB) induced proliferation in the TF 1 human erythroleukemic cell line. The Neutralization Dose (ND₅₀) for this effect is typically < 3.00 µg/mL in the presence of 10 ng/mL Recombinant Human IL-13. " class="big_lightbox"> 213-ILB) induced proliferation in the TF 1 human erythroleukemic cell line. The Neutralization Dose (ND₅₀) for this effect is typically < 3.00 µg/mL in the presence of 10 ng/mL Recombinant Human IL-13. " alt="Human IL-13 Antibody (Catalog # AF-213-NA) neutralizes Recombinant Human IL-13 (213-ILB) induced proliferation in the TF 1 human erythroleukemic cell line. The Neutralization Dose (ND₅₀) for this effect is typically < 3.00 µg/mL in the presence of 10 ng/mL Recombinant Human IL-13. " class="big_thumb" />

213-ILB) was serially diluted and captured by Mouse Anti-Human IL‑13 Monoclonal Antibody (Catalog # MAB213) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-213-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 213-ILB) was serially diluted and captured by Mouse Anti-Human IL‑13 Monoclonal Antibody (Catalog # MAB213) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-213-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

204-IL) and 10 µg/mL Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for 3 days were stained with Mouse Anti-Human IL-13 Monoclonal Antibody (Catalog # MAB2131) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P). Quadrant markers were set based on control antibody staining (Catalog # MAB002)." class="big_lightbox"> 204-IL) and 10 µg/mL Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for 3 days were stained with Mouse Anti-Human IL-13 Monoclonal Antibody (Catalog # MAB2131) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P). Quadrant markers were set based on control antibody staining (Catalog # MAB002)." alt="Human peripheral blood mononuclear cells (PBMCs) treated with 5 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 10 µg/mL Goat Anti-Human IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-285-NA) for 3 days were stained with Mouse Anti-Human IL-13 Monoclonal Antibody (Catalog # MAB2131) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P). Quadrant markers were set based on control antibody staining (Catalog # MAB002)." class="big_thumb" />

213-ILB) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-13 (10 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-Human IL-13 Monoclonal Antibody (Catalog # MAB2131). The ND₅₀ is typically 1-4 µg/mL." class="big_lightbox"> 213-ILB) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-13 (10 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-Human IL-13 Monoclonal Antibody (Catalog # MAB2131). The ND₅₀ is typically 1-4 µg/mL." class="big_thumb" />

1945-RL), Recombinant Human IL-13 (Catalog # 213-ILB) and Recombinant Mouse IL-13 (Catalog # 413-ML). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Rat IL-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1945) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-13 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox"> 1945-RL), Recombinant Human IL-13 (Catalog # 213-ILB) and Recombinant Mouse IL-13 (Catalog # 413-ML). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Rat IL-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1945) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-13 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Rat IL-13 (Catalog # 1945-RL), Recombinant Human IL-13 (Catalog # 213-ILB) and Recombinant Mouse IL-13 (Catalog # 413-ML). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Rat IL-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1945) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-13 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

1945-RL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-13 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1945). The ND₅₀ is typically 0.2-0.8 µg/mL." class="big_lightbox"> 1945-RL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-13 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1945). The ND₅₀ is typically 0.2-0.8 µg/mL." class="big_thumb" />

413-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-13 Polyclonal Antibody (Catalog # AB-413-NA). The ND₅₀ is typically 15-30 µg/mL." class="big_lightbox"> 413-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-13 Polyclonal Antibody (Catalog # AB-413-NA). The ND₅₀ is typically 15-30 µg/mL." alt="Recombinant Mouse IL-13 (Catalog # 413-ML) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-13 Polyclonal Antibody (Catalog # AB-413-NA). The ND₅₀ is typically 15-30 µg/mL." class="big_thumb" />

1945-RL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑13 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL‑13 Monoclonal Antibody (Catalog # MAB1945). The ND₅₀ is typically 1-4 µg/mL." class="big_lightbox"> 1945-RL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑13 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL‑13 Monoclonal Antibody (Catalog # MAB1945). The ND₅₀ is typically 1-4 µg/mL." alt="Recombinant Rat IL‑13 (Catalog # 1945-RL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL‑13 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat IL‑13 Monoclonal Antibody (Catalog # MAB1945). The ND₅₀ is typically 1-4 µg/mL." class="big_thumb" />