IL-12/IL-23 p40 Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-12/IL-23 p40 Antibody [Biotin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑12 p40
  Met23-Ser355
  Accession # P43432
• Specificity: Detects mouse IL-12 p40 in ELISAs and Western blots. In sandwich immunoassays, approximately 30% cross-reactivity with recombinant mouse IL‑12 p70 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Il12b
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • ELISA Standard (Matched Pair)
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: Mouse IL-12/IL-23 p40 Allele-specific Sandwich Immunoassay
  ELISA Capture:Mouse IL-12/IL-23 p40 Antibody (Catalog # MAB499)
  ELISA Detection:Mouse IL-12/IL-23 p40 Biotinylated Antibody (Catalog # BAF499)
  Standard: Recombinant Mouse IL-12/IL-23 p40 Homodimer (Catalog # 499-ML)
  
  Mouse IL-12/IL-23 p40 Nonallele-specific Sandwich Immunoassay
  ELISA Capture: Mouse IL-12/IL-23 p40 Antibody (Catalog # MAB4991)
  ELISA Detection: Mouse IL-12/IL-23 p40 Biotinylated Antibody (Catalog # BAF499)
  Standard: Recombinant Mouse IL-12/IL-23 p40 Homodimer (Catalog # 499-ML)
  
  Mouse IL-23 Sandwich Immunoassay
  ELISA Capture: Mouse IL-23 Antibody (Catalog # MAB1887)
  ELISA Detection: Mouse IL-12/IL-23 p40 Biotinylated Antibody (Catalog # BAF499)
  Standard: Recombinant Mouse IL-23 (Catalog # 1887-ML)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12/IL-23 p40 Antibody [Biotin]

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL12 p40
• IL-12 p40
• IL-12 subunit p40
• IL12B
• IL-12B
• IL-12BNK cell stimulatory factor chain 2
• interleukin 12, p40
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
• interleukin-12 beta chain
• interleukin-12 subunit beta
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2
• NKSF2IL12, subunit p40

Background

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 has multiple effects on T cells and NK cells and is a key mediator in the development of Th1 cells. IL-12 is a heterodimeric cytokine containing two disulfide-linked subunits, p35 and p40. Human and mouse IL-12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. Although mouse IL-12 is active on human or mouse IL-12 responsive cells, human IL-12 is not active on mouse cells. The disulfide-linked mouse p40 homodimer can bind to IL-12 receptors and is an antagonist of IL-12 activities in vitro. The mouse p40 monomer is at least ten times less active than the homodimer as an IL-12 antagonist. At the present time, the existence and the physiological role of mouse p40 homodimer in vivo remains to be determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-12/IL-23 p40 Antibody (BAF499)(8)

Publications using BAF499

• Seon-Yeong Lee, Su-Jin Moon, Young-Mee Moon, Hyeon-Beom Seo, Jun-Geol Ryu, A Ram Lee et al. A novel cytokine consisting of the p40 and EBI3 subunits suppresses experimental autoimmune arthritis via reciprocal regulation of Th17 and Treg cells Cellular & Molecular Immunology 2022-01-01 [PMID: 34782759] (ELISA Detection, Human, Mouse)
• J Georgy, Y Arlt, JM Moll, M Ouzin, HT Weitz, L Gremer, D Willbold, J Grötzinger, F Thives-Kur, J Scheller, DM Floss Tryptophan (W) at position 37 of murine IL-12/IL-23 p40 is mandatory for binding to IL-12Rbeta1 and subsequent signal transduction The Journal of Biological Chemistry, 2021-10-09;0(0):101295. 2021-10-09 [PMID: 34637790] (Western Blot, Mouse)
• A widespread sequence-specific mRNA decay pathway mediated by hnRNPs A1 and A2/B1 Genes Dev, 2016-05-01;30(9):1070-85. 2016-05-01 [PMID: 27151978] (Western Blot, Human)
• Flores R, Kim E, Zhou L, Yang C, Zhao J, Gambotto A, Robbins P IL-Y, a synthetic member of the IL-12 cytokine family, suppresses the development of type 1 diabetes in NOD mice. Eur J Immunol, 2015-09-01;45(11):3114-25. 2015-09-01 [PMID: 26260044] (Direct ELISA, Mouse)
• Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production. Proc. Natl. Acad. Sci. U.S.A., 2009-03-16;106(14):5954-9. 2009-03-16 [PMID: 19289819] (ELISA Development, Mouse)
• An HJ, Jeong HJ, Um JY, Kim HM, Hong SH Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages. J Ethnopharmacol, 2006-03-10;106(3):418-24. 2006-03-10 [PMID: 16530364] (ELISA Development, Mouse)
• Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells. Blood, 2004-05-13;104(5):1404-10. 2004-05-13 [PMID: 15142882] (ELISA Development, Human)
• Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity. Am. J. Pathol., 2002-04-01;160(4):1353-60. 2002-04-01 [PMID: 11943720] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: Il12b
• Uniprot:
  • P43432()