IL-12/IL-23 p40 Antibodies

419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-12/IL-23 p40 Monoclonal Antibody (Catalog # MAB4991). The ND₅₀ is typically 0.04-0.3 µg/mL." class="big_lightbox"> 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-12/IL-23 p40 Monoclonal Antibody (Catalog # MAB4991). The ND₅₀ is typically 0.04-0.3 µg/mL." alt="Recombinant Mouse IL-12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-12/IL-23 p40 Monoclonal Antibody (Catalog # MAB4991). The ND₅₀ is typically 0.04-0.3 µg/mL." class="big_thumb" />

499-ML) was serially diluted and captured by Rat Anti-Mouse IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB4991) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑12/IL‑23 p40 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox"> 499-ML) was serially diluted and captured by Rat Anti-Mouse IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB4991) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑12/IL‑23 p40 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

402-ML), Recombinant Human IL-23 (Catalog # 1290-IL) stimulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Human IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-12/IL-23 p40 Monoclonal Antibody (Catalog # MAB1510). The ND₅₀ is typically 0.02-0.1 µg/mL. This antibody also neutralizes Human IL-12 induced activity in human PBLs. The ND50 is approximately 0.05-0.25 µg/mL in the presence of 1 ng/mL Recombinant Human IL-12." class="big_lightbox"> 402-ML), Recombinant Human IL-23 (Catalog # 1290-IL) stimulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Human IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-12/IL-23 p40 Monoclonal Antibody (Catalog # MAB1510). The ND₅₀ is typically 0.02-0.1 µg/mL. This antibody also neutralizes Human IL-12 induced activity in human PBLs. The ND50 is approximately 0.05-0.25 µg/mL in the presence of 1 ng/mL Recombinant Human IL-12." alt="In the presence of Recombinant Mouse IL-2 (10 ng/mL, Catalog # 402-ML), Recombinant Human IL-23 (Catalog # 1290-IL) stimulates IL-17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL-17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL-17 secretion elicited by Recombinant Human IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-12/IL-23 p40 Monoclonal Antibody (Catalog # MAB1510). The ND₅₀ is typically 0.02-0.1 µg/mL. This antibody also neutralizes Human IL-12 induced activity in human PBLs. The ND50 is approximately 0.05-0.25 µg/mL in the presence of 1 ng/mL Recombinant Human IL-12." class="big_thumb" />

1954-FL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL-12 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Feline IL-12/IL-23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1954). The ND₅₀ is typically 0.5-2.0 µg/mL." class="big_lightbox"> 1954-FL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL-12 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Feline IL-12/IL-23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1954). The ND₅₀ is typically 0.5-2.0 µg/mL." alt="Recombinant Feline IL-12 (Catalog # 1954-FL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL-12 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Feline IL-12/IL-23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1954). The ND₅₀ is typically 0.5-2.0 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF019). A specific band was detected for IL-12/IL-23 p40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox"> HAF019). A specific band was detected for IL-12/IL-23 p40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of Hs 294T human melanoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human IL-12/IL-23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF309) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IL-12/IL-23 p40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

1290-IL) induces IL-17 secretion in the mouse splenocytes in a dose-dependent manner (orange line), as measured by Mouse IL-17 Quantikine kit. IL-17 Secretion elicited by Recombinant Human IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-12/IL-23 p40 Anti-gen Affinity-purified Polyclonal Antibody (Catalog # AF309). The ND₅₀ is typically 0.004-0.02 µg/mL." class="big_lightbox"> 1290-IL) induces IL-17 secretion in the mouse splenocytes in a dose-dependent manner (orange line), as measured by Mouse IL-17 Quantikine kit. IL-17 Secretion elicited by Recombinant Human IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-12/IL-23 p40 Anti-gen Affinity-purified Polyclonal Antibody (Catalog # AF309). The ND₅₀ is typically 0.004-0.02 µg/mL." class="big_thumb" />

499-ML) was serially diluted and captured by Rat Anti-Mouse IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB499) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑12/IL‑23 p40 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox"> 499-ML) was serially diluted and captured by Rat Anti-Mouse IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB499) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑12/IL‑23 p40 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" alt="Recombinant Mouse IL‑12/IL‑23 p40 Homodimer (Catalog # 499-ML) was serially diluted and captured by Rat Anti-Mouse IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB499) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑12/IL‑23 p40 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

309-IL) was serially diluted and captured by Mouse Anti-Human IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB609) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 309-IL) was serially diluted and captured by Mouse Anti-Human IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB609) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human IL‑12/IL‑23 p40 Monomer (Catalog # 309-IL) was serially diluted and captured by Mouse Anti-Human IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB609) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

912-PL) was serially diluted and captured by Mouse Anti-Porcine IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB9121) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Porcine IL‑12/IL‑23 p40 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox"> 912-PL) was serially diluted and captured by Mouse Anti-Porcine IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB9121) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Porcine IL‑12/IL‑23 p40 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" alt="Recombinant Porcine IL‑12 (Catalog # 912-PL) was serially diluted and captured by Mouse Anti-Porcine IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB9121) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Porcine IL‑12/IL‑23 p40 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

2118-CL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-12 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Canine IL-12/IL-23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1969). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_lightbox"> 2118-CL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-12 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Canine IL-12/IL-23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1969). The ND₅₀ is typically 0.1-0.4 µg/mL." alt="Recombinant Canine IL-12 (2118-CL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-12 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Canine IL-12/IL-23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1969). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_thumb" />

VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="IL‑12/IL‑23 p40 was detected in immersion fixed paraffin-embedded sections of Mouse Spleen using Rat Anti-MouseIL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB6092) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />