IL-12/IL-23 p40 Antibody (116211) [Biotin]

• Reactivity: Po
• Applications: ELISA(Det)
• Clone: 116211
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: LYOPH

IL-12/IL-23 p40 Antibody (116211) [Biotin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant porcine IL‑12 p70 heterodimer
• Specificity: Detects the p40 subunit of porcine IL-12 and IL-23. In ELISAs, this antibody shows 25-35% cross-reactivity with recombinant human (rh) IL‑12 and rhIL‑23 and no cross-reactivity with rmIL-12 or rmIL-23.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Porcine IL-12/IL-23 p40 Antibody (Catalog # MAB9121)
  ELISA Detection: Porcine IL-12/IL-23 p40 Biotinylated Antibody (Catalog # BAM9122)
  Standard: Recombinant Porcine IL-12 (Catalog # 912-PL)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12/IL-23 p40 Antibody (116211) [Biotin]

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL12 p40
• IL-12 p40
• IL-12 subunit p40
• IL12B
• IL-12B
• IL-12BNK cell stimulatory factor chain 2
• interleukin 12, p40
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
• interleukin-12 beta chain
• interleukin-12 subunit beta
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2
• NKSF2IL12, subunit p40

Background

IL-12 and IL-23 are disulfide-linked heterodimers consisting of a p40 subunit that is common to both cytokines and a unique subunit, p19 in IL-23 or p35 in IL-12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-12/IL-23 p40 Antibody (BAM9122)(2)

Publications using BAM9122

• S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets Infect. Immun., 2017-03-23;85(4):. 2017-03-23 [PMID: 28167667] (ELISA Development (Detection), Porcine)
• Andrea Ladinig, Joan K Lunney, Carlos J H Souza, Carolyn Ashley, Graham Plastow, John C S Harding Cytokine profiles in pregnant gilts experimentally infected with porcine reproductive and respiratory syndrome virus and relationships with viral load and fetal outcome Veterinary Research 2014-12-06 [PMID: 25479904]