IL-11R alpha Antibody (473143) [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clone: 473143
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-11R alpha Antibody (473143) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-11 R alpha
  Cys26-Val363
  Accession # Q14626
• Specificity: Detects human IL-11 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with rmIL-11 R alpha or recombinant human IL‑6 R alpha is observed. In Western blots, 25% cross-reactivity with recombinant mouse (rm) IL-11 R alpha is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL11RA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-11R alpha Antibody (473143) [Unconjugated]

• AI314697
• GP130
• IL-11 R alpha
• IL11R alpha
• IL11RA
• IL-11Ra
• Il11ra2
• NR1

Background

Human interleukin 11 receptor alpha (IL-11 R alpha ) is 150 kDa type I transmembrane protein in the hematopoietin cytokine receptor family that was cloned on the basis of its conserved WSXWS motif. IL-11 R alpha cDNA encodes a 422 amino acid (aa) residue precursor type I membrane protein, containg a signal peptide followed by extracellular (ECD), transmembrane and cytoplasmic domains. The extracellular region which is closely related to that of IL-6 R alpha and CNTF R alpha contains an immunoglobulin-like domain and two fibronectin-type III-like domains. Within the ECD, human IL-11 R alpha shares 85% and 83% aa sequence identity with mouse and rat IL-11 R alpha , respectively (1‑3). Alternate splicing generates an isoform of human IL-11 R alpha which lacks the cytoplasmic domain. By itself, IL-11 R alpha binds IL-11 with low affinity. IL-11 R alpha , together with gp130, forms a functional high-affinity receptor complex for IL-11. The expression of IL-11 R alpha has been detected in all adult tissues examined (lung, stomach, intestine), during embryonic development and in totipotent and differentiating embryonic stem cells. Recombinant soluble IL-11 R alpha has been shown to mediate IL-11 responsiveness in cells expressing the gp130 molecule. In cells expressing transmembrane IL-11 R alpha and gp130, soluble IL-11 R alpha has been shown to act as an IL-11 antagonist (3‑6).

1. Cherel, M. et al. (1995) Blood 86:2534.
2. Schleinkofer, K. et al. (2001) J. Mol. Biol. 306:263.
3. Nandurkar, H.H. et al. (1996) Oncogene 12:585.
4. Taga, T. et al. (1997) Annu. Rev. Immuno. 15:797.
5. Davidson, A.J. et al. (1997) Stem Cells 15:119.
6. Curtis, D.J. et al. (1997) Blood 90:4403.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-11R alpha Antibody (MAB1977)(4)

Publications using MAB1977

• S Schafer, S Viswanatha, AA Widjaja, WW Lim, A Moreno-Mor, DM DeLaughter, B Ng, G Patone, K Chow, E Khin, J Tan, SP Chothani, L Ye, OJL Rackham, NSJ Ko, NE Sahib, CJ Pua, NTG Zhen, C Xie, M Wang, H Maatz, S Lim, K Saar, S Blachut, E Petretto, S Schmidt, T Putoczki, N Guimarães-, H Wakimoto, S van Heesch, K Sigmundsso, SL Lim, JL Soon, VTT Chao, YL Chua, TE Tan, SM Evans, YJ Loh, MH Jamal, KK Ong, KC Chua, BH Ong, MJ Chakaramak, JG Seidman, CE Seidman, N Hubner, KYK Sin, SA Cook IL-11 is a crucial determinant of cardiovascular fibrosis Nature, 2017-11-13;552(7683):110-115. 11/13/2017 [PMID: 29160304]
• Andriy Marusyk, Doris P. Tabassum, Philipp M. Altrock, Vanessa Almendro, Franziska Michor, Kornelia Polyak Non-cell-autonomous driving of tumour growth supports sub-clonal heterogeneity Nature 10/2/2014 [PMID: 25079331]
• Yan , Dongyao, Kc , Ranjan, Chen , Di, Xiao , Guozhi, Im , Hee-Jeon Bovine lactoferricin-induced anti-inflammation is, in part, via up-regulation of interleukin-11 by secondary activation of STAT3 in human articular cartilage. J Biol Chem, 2013-09-13;288(44):31655-69. 2013-09-13 [PMID: 24036113] (Western Blot, Human)
• Nieminen P, Morgan NV, Fenwick AL, Parmanen S, Veistinen L, Mikkola ML, van der Spek PJ, Giraud A, Judd L, Arte S, Brueton LA, Wall SA, Mathijssen IM, Maher ER, Wilkie AO, Kreiborg S, Thesleff I Inactivation of IL11 signaling causes craniosynostosis, delayed tooth eruption, and supernumerary teeth. Am. J. Hum. Genet., 2011-07-15;89(1):67-81. 2011-07-15 [PMID: 21741611] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: IL11RA
• Uniprot:
  • Q14626()