IL-11R alpha Antibody (473143) [Alexa Fluor™ Plus 594]

• Reactivity: Hu
• Clone: 473143
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 594

IL-11R alpha Antibody (473143) [Alexa Fluor™ Plus 594] Summary

• Specificity: Detects human IL-11 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with rmIL-11 R alpha or recombinant human IL‑6 R alpha is observed. In Western blots, 25% cross-reactivity with recombinant mouse (rm) IL-11 R alpha is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-11R alpha Antibody (473143) [Alexa Fluor™ Plus 594]

• AI314697
• GP130
• IL-11 R alpha
• IL11R alpha
• IL11RA
• IL-11Ra
• Il11ra2
• NR1

Background

Human interleukin 11 receptor alpha (IL-11 R alpha ) is 150 kDa type I transmembrane protein in the hematopoietin cytokine receptor family that was cloned on the basis of its conserved WSXWS motif. IL-11 R alpha cDNA encodes a 422 amino acid (aa) residue precursor type I membrane protein, containg a signal peptide followed by extracellular (ECD), transmembrane and cytoplasmic domains. The extracellular region which is closely related to that of IL-6 R alpha and CNTF R alpha contains an immunoglobulin-like domain and two fibronectin-type III-like domains. Within the ECD, human IL-11 R alpha shares 85% and 83% aa sequence identity with mouse and rat IL-11 R alpha , respectively (1‑3). Alternate splicing generates an isoform of human IL-11 R alpha which lacks the cytoplasmic domain. By itself, IL-11 R alpha binds IL-11 with low affinity. IL-11 R alpha , together with gp130, forms a functional high-affinity receptor complex for IL-11. The expression of IL-11 R alpha has been detected in all adult tissues examined (lung, stomach, intestine), during embryonic development and in totipotent and differentiating embryonic stem cells. Recombinant soluble IL-11 R alpha has been shown to mediate IL-11 responsiveness in cells expressing the gp130 molecule. In cells expressing transmembrane IL-11 R alpha and gp130, soluble IL-11 R alpha has been shown to act as an IL-11 antagonist (3‑6).

1. Cherel, M. et al. (1995) Blood 86:2534.
2. Schleinkofer, K. et al. (2001) J. Mol. Biol. 306:263.
3. Nandurkar, H.H. et al. (1996) Oncogene 12:585.
4. Taga, T. et al. (1997) Annu. Rev. Immuno. 15:797.
5. Davidson, A.J. et al. (1997) Stem Cells 15:119.
6. Curtis, D.J. et al. (1997) Blood 90:4403.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.