IL-11R alpha Antibody (473143) [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB
• Clone: 473143
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

IL-11R alpha Antibody (473143) [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-11 Rα
  Cys26-Val363
  Accession # Q14626
• Specificity: Detects human IL-11 R alpha in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with rmIL-11 R alpha or recombinant human IL‑6 R alpha is observed. In Western blots, 25% cross-reactivity with recombinant mouse (rm) IL-11 R alpha is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-11R alpha Antibody (473143) [Alexa Fluor® 750]

• AI314697
• GP130
• IL-11 R alpha
• IL11R alpha
• IL11RA
• IL-11Ra
• Il11ra2
• NR1

Background

Human interleukin 11 receptor alpha (IL-11 R alpha ) is 150 kDa type I transmembrane protein in the hematopoietin cytokine receptor family that was cloned on the basis of its conserved WSXWS motif. IL-11 R alpha cDNA encodes a 422 amino acid (aa) residue precursor type I membrane protein, containg a signal peptide followed by extracellular (ECD), transmembrane and cytoplasmic domains. The extracellular region which is closely related to that of IL-6 R alpha and CNTF R alpha contains an immunoglobulin-like domain and two fibronectin-type III-like domains. Within the ECD, human IL-11 R alpha shares 85% and 83% aa sequence identity with mouse and rat IL-11 R alpha , respectively (1‑3). Alternate splicing generates an isoform of human IL-11 R alpha which lacks the cytoplasmic domain. By itself, IL-11 R alpha binds IL-11 with low affinity. IL-11 R alpha , together with gp130, forms a functional high-affinity receptor complex for IL-11. The expression of IL-11 R alpha has been detected in all adult tissues examined (lung, stomach, intestine), during embryonic development and in totipotent and differentiating embryonic stem cells. Recombinant soluble IL-11 R alpha has been shown to mediate IL-11 responsiveness in cells expressing the gp130 molecule. In cells expressing transmembrane IL-11 R alpha and gp130, soluble IL-11 R alpha has been shown to act as an IL-11 antagonist (3‑6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.