IL-10 Antibody (JES052A5) [Unconjugated]

Western blot shows 25 ng of Recombinant Mouse IL-10 (Catalog # 417-ML), Recombinant Human IL-10 (Catalog # 217-IL), Recombinant Rat IL-10 (Catalog # 522-RLB), and Recombinant Human IL-26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-10 Monoclonal Antibody (Catalog # MAB417) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-10 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Recombinant Mouse IL‑10 (Catalog # 417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (2.5 ng/mL) is neutralized (green line) by increasing concen­trations of Rat Anti-Mouse IL‑10 Monoclonal Antibody (Catalog # MAB417). The ND50 is typically 0.005-0.015 µg/mL.

Imipramine downregulates an M2-like program in TAMs(A) Western blot analysis of ARG1 and IL-10 in single tumors treated or not with IM or anti-VEGF.(B) Flow cytometry analysis of ARG1 and IL-10 expression in GBM tumors treated for 1 week. Ctrl (n = 9 tumors), IM (n = 6), anti-VEGF (n = 6), anti-VEGFR2 (n = 6), Axitinib (n = 6). Macrophages were gated as CD45+CD11b+Ly6C-Ly6G−.(C) Expression of ARG1 and IL-10 in microglia (CD49d−) and MDMs (CD49d+) assessed by FACS in untreated tumors (n = 5).(D) Expression of MHC-II within microglia as assessed by flow cytometry. Ctrl (n = 4 tumors) and IM + anti-VEGF (n = 4).(E) Ex vivo co-cultures of tumoral CD11b cells and activated splenic CFSE-labeled CD8 or CD4 T cells. Each dot represents the average of two or three technical replicates. T cells alone (n = 4), Ctrl co-culture (n = 5), anti-VEGF (n = 3), IM (n = 4), IM + anti-VEGF (n = 4).(F) Analysis of the M2-like program in cytokine-polarized macrophages as assessed by qRT-PCR analysis of Ctrl and IM-treated M2-like BMDMs. Expression is normalized to 18S statistics by Welch’s t test. Each dot represents an individual sample. Data are representative of three independent experiments.(G) Analysis of the M1-like program in BMDMs assessed by FACS. Each dot represents an individual replicate.(H) Expression of Hrh1 mRNA normalized to 18S in ex vivo M1-and M2-polarized BMDMs, either untreated or IM treated for 24 h. Each dot represents an individual sample. Data are representative of three independent experiments.(I) mRNA expression of Hrh1 in FACS-sorted microglia or MDMs from Ctrl and IM-treated tumors.(J) mRNA expression of Arg1, Chil3, and Il10 in M2-polarized macrophages that were transfected with siCtrl or two different siHrh1 constructs. Cells were treated with 40 μm IM for 24 h. Data are representative of two independent experiments.(K) Western blot analysis of MRC1 and ARG1 expression of siRNA-transfected M2 BMDMs. Data are representative of two independent experiments.(L) CD8 and CD4 T cell proliferation during co-culture with tumoral CD11b cells isolated from untreated (n = 2) or TFP-treated tumors (n = 4).(M) mRNA expression of Hrh1, Arg1, and MMP2 in CD11b cells isolated from tumors treated with IM (n = 4), TFP (n = 4), or untreated Ctrl (n = 5).(N) Phagocytosis assay involving sorted microglia and MDMs from untreated or IM-treated tumors assayed with green pHrodo S. aureus bioparticles. Data presented as mean fluorescence intensity (MFI) of pHrodo/live cells. (Para break) Data in all quantitative panels are presented as mean ± SEM ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001; ns, no statistical significance. Statistical analysis by Mann-Whitney test or one-way ANOVA, unless otherwise stated. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36113478), licensed under a CC-BY license. Not internally tested by R&D Systems.

Lymphocyte infiltrate in tumors from IL-10 deficient mice (A, C and D) or from anti-IL10/IL-10R neutralizing antibody treated mice (B, E and F). A. Single cell suspensions were stained with the indicated antibodies plus anti-CD45 and anti-F4/80. Lymphocytes were analyzed in the CD45+F4/80- population. B. After 6 hours incubation in tissue culture flasks, non adherent cells from total tumor suspension were harvested and stained with the indicated antibodies, tetramer and anti-CD45. C and E. Immunofluorescence of cryo-sections from tumors harvested from IL-10 KO and wild type mice or neutralizing anti-IL10/IL-1R and irrelevant IgG antibodies, as indicated. Sections were stained with anti-CD8 (in FITC, green) and PE (red) conjugated HPV16 E749-57 tetramer - E749-57 Tet, and irrelevant tetramer - Irrelevant Tet. Cells were counterstained with DAPI (blue). Arrowheads indicate double positive cells in yellow, arrows indicate cells positive only for CD8. D and F. Granzyme B expression in tumor cryo-sections from IL-10 KO and wild type mice or mice treated with anti-IL10/IL-10R neutralizing antibodies and irrelevant IgG, as indicated. Sections were stained with anti-CD8 (green), followed by fixation, permeabilization and staining with anti-Granzyme B (red). Tissue was counterstained with DAPI. Solid arrows indicate double positive cells, arrowhead indicate CD8 only positive cell. Insets show details of cells positive only for CD8 (green) or double positive CD8/tetramer (C and E), CD8/Granzyme B (D and F) in yellow or orange due to the sobreposition of emission from FITC and PE (tetramer) or alexa 594 (Granzyme B). Image collected and cropped by CiteAb from the following publication (//bmcimmunol.biomedcentral.com/articles/10.1186/1471-2172-11-27), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, B/N, ELISA(Cap)
• Clone: JES052A5
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IL-10 Antibody (JES052A5) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse IL-10
  Accession # P18893
• Specificity: Detects mouse IL-10 in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 8% cross-reactivity with recombinant rat IL-10 and 1% cross-reactivity with recombinant cotton rat IL‑10 is observed. In sandwich ELISAs, no cross-reactivity with recombinant human (rh) IL-10, recombinant mouse IL-10 R, rhIL‑10 R, recombinant canine IL-10, recombinant feline IL-10, or recombinant porcine IL-10 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: IL10
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.005-0.015 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Mouse IL-10 Biotinylated Antibody (Catalog number BAF417)
  Standard: Recombinant Mouse IL-10 (Catalog number 417-ML)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10 Antibody (JES052A5) [Unconjugated]

• CSIF
• CSIFMGC126450
• Cytokine synthesis inhibitory factor
• GVHDS
• IL10
• IL-10
• IL10A
• IL-10MGC126451
• interleukin 10
• interleukin-10
• TGIF

Background

Interleukin 10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-10 Antibody (MAB417)(61)

Publications using MAB417

• Blanco, T;Nakagawa, H;Musayeva, A;Krauthammer, M;Singh, RB;Narimatsu, A;Ge, H;Shoushtari, SI;Dana, R; Acquired Immunostimulatory Phenotype of Migratory CD103+ Dendritic Cells Promotes Alloimmunity Following Corneal Transplantation JCI insight 2024-09-05 [PMID: 39235864] (Bioassay, Mouse)
• Gallant, RM;Snyder, JM;Ayres, JS; Fluoxetine promotes immunometabolic defenses to mediate host-pathogen cooperation during sepsis bioRxiv : the preprint server for biology 2023-11-18 [PMID: 38013994] (ELISA Capture, Mouse)
• Zhang, M;Chen, J;Zhang, H;Dong, H;Yue, Y;Wang, S; Interleukin-10 increases macrophage-mediated chemotherapy resistance via FABP5 signaling in multiple myeloma International immunopharmacology 2023-09-02 [PMID: 37666065] (Neutralization, Mouse)
• Manish Mishra, Manisha Yadav, Sandeep Kumar, Raj Kumar, Pradip Sen TIM-3 increases the abundance of type-2 dendritic cells during Leishmania donovani infection by enhancing IL-10 production via STAT3 Cell Death & Disease 5/18/2023 [PMID: 37202419]
• K Kasahara, N Sasaki, HZ Amin, T Tanaka, S Horibe, T Yamashita, KI Hirata, Y Rikitake Depletion of Foxp3+ regulatory T cells augments CD4+ T cell immune responses in atherosclerosis-prone hypercholesterolemic mice Heliyon, 2022-07-19;8(7):e09981. 2022-07-19 [PMID: 35898604] (ELISA Capture, Mouse)
• Myles R. McCrary, Michael Q. Jiang, Kaleena Jesson, Xiaohuan Gu, Meghan T. Logun, Anika Wu et al. Glycosaminoglycan scaffolding and neural progenitor cell transplantation promotes regenerative immunomodulation in the mouse ischemic brain Experimental Neurology 2022-11-01 [PMID: 35868359] (Neutralization, Mouse)
• Myles R. McCrary, Michael Q. Jiang, Kaleena Jesson, Xiaohuan Gu, Meghan T. Logun, Anika Wu et al. Glycosaminoglycan scaffolding and neural progenitor cell transplantation promotes regenerative immunomodulation in the mouse ischemic brain Experimental Neurology 11/1/2022 [PMID: 35868359]
• Ding Jianyi, Gan Haili, Yin Bo, Yang Meiqin, Huang Baoyou, Hu Haoran et al. Myeloid-derived suppressor cells cross-talk with B10 cells by BAFF/BAFF-R pathway to promote immunosuppression in cervical cancer Cancer Immunology, Immunotherapy 1/1/2023 [PMID: 35725835]
• Lim SW, Shin YJ, Cui S et al. Therapeutic effect of multiple functional minicircle vector encoding anti-CD25/IL-10/CXCR3 in allograft rejection model The Korean journal of internal medicine 6/21/2022 [PMID: 35725307]
• Z Liang, P He, Y Han, CC Yun Survival of stem cells and progenitors in the intestine is regulated by LPA5-dependent signaling Cellular and Molecular Gastroenterology and Hepatology, 2022-04-04;0(0):. 2022-04-04 [PMID: 35390517] (Tissue Culture, Mouse)
• N Honke, T Lowin, B Opgenoorth, N Shaabani, A Lautwein, JR Teijaro, M Schneider, G Pongratz Endogenously produced catecholamines improve the regulatory function of TLR9-activated B cells PloS Biology, 2022-01-24;20(1):e3001513. 2022-01-24 [PMID: 35073310] (Flow Cytometry, Mouse)
• Q Wu, S Xie, Y Zhu, J Chen, J Tian, S Xiong, C Wu, Y Ye, Y Peng Wogonin Strengthens the Therapeutic Effects of Mesenchymal Stem Cells in DSS-Induced Colitis via Promoting IL-10 Production Oxidative Medicine and Cellular Longevity, 2021-06-17;2021(0):5527935. 2021-06-17 [PMID: 34239686] (Neutralization, Mouse)
• G Hoeffel, G Debroas, A Roger, R Rossignol, J Gouilly, C Laprie, L Chasson, PV Barbon, A Balsamo, A Reynders, A Moqrich, S Ugolini Sensory�neuron-derived TAFA4 promotes macrophage tissue repair functions Nature, 2021-05-19;0(0):. 2021-05-19 [PMID: 34012116] (Neutralization, Mouse)
• L Radomir, MP Kramer, M Perpinial, N Schottlend, S Rabani, K David, A Wiener, H Lewinsky, S Becker-Her, R Aharoni, R Milo, C Mauri, I Shachar The survival and function of IL-10-producing regulatory B cells are negatively controlled by SLAMF5 Nature Communications, 2021-03-25;12(1):1893. 2021-03-25 [PMID: 33767202] (Neutralization, Mouse)
• Q Li, X Lan, X Han, F Durham, J Wan, A Weiland, RC Koehler, J Wang Microglia-derived interleukin-10 accelerates post-intracerebral hemorrhage hematoma clearance by regulating CD36 Brain, Behavior, and Immunity, 2021-02-13;94(0):437-457. 2021-02-13 [PMID: 33588074] (Neutralization, Mouse)
• Daisuke Ikutame, Kentaro Urata, Tatsuki Oto, Shintaro Fujiwara, Toshimitsu Iinuma, Ikuko Shibuta et al. Aging-Related Phenotypic Conversion of Medullary Microglia Enhances Intraoral Incisional Pain Sensitivity International Journal of Molecular Sciences 10/23/2020 [PMID: 33114176]
• Mahmoud Elashiry, Mohamed M. Elashiry, Ranya Elsayed, Mythily Rajendran, Carol Auersvald, Rana Zeitoun et al. Dendritic cell derived exosomes loaded with immunoregulatory cargo reprogram local immune responses and inhibit degenerative bone disease in vivo Journal of Extracellular Vesicles 9/1/2020 [PMID: 32944183]
• YR Na, JW Kwon, DY Kim, H Chung, J Song, D Jung, H Quan, D Kim, JS Kim, YW Ju, W Han, HS Ryu, YS Lee, JJ Hong, SH Seok Protein Kinase A Catalytic Subunit Is a Molecular Switch that Promotes the Pro-tumoral Function of Macrophages Cell Rep, 2020-05-12;31(6):107643. 2020-05-12 [PMID: 32402274] (IHC, Mouse)
• Y Zhao, J Wang, Z Min, L Peng, S Qi, W Lin I-alowCD11bhigh DC Regulates the Immune Response in the Eyes of Experimental Autoimmune Uveitis Mediators Inflamm., 2020-03-10;2020(0):6947482. 2020-03-10 [PMID: 32256194] (Neutralization, Mouse)
• B Wang, S Wu, Z Ma, T Wang, C Yang BMSCs pre-treatment ameliorates inflammation-related tissue destruction in LPS-induced rat DIC model Cell Death Dis, 2018-10-03;9(10):1024. 2018-10-03 [PMID: 30282969] (Neutralization, Rat)
• Victoria L. Palmer, Alexandra N. Worth, Robyn L. Scott, Greg A. Perry, Mei Yan, Quan-Zhen Li et al. IL10 restrains autoreactive B cells in transgenic mice expressing inactive RAG1 Cellular Immunology 9/1/2018 [PMID: 30017086]
• L Tian, S Song, B Zhu, S Liu Electroacupuncture at ST-36 Protects Interstitial Cells of Cajal via Sustaining Heme Oxygenase-1 Positive M2 Macrophages in the Stomach of Diabetic Mice Oxid Med Cell Longev, 2018-04-26;2018(0):3987134. 2018-04-26 [PMID: 29854081] (Western Blot, Mouse)
• M William, LP Leroux, V Chaparro, J Lorent, TE Graber, MN M'Boutchou, T Charpentie, A Fabié, CM Dozois, S Stäger, LC van Kempen, T Alain, O Larsson, M Jaramillo eIF4E-Binding Proteins 1 and 2 Limit Macrophage Anti-Inflammatory Responses through Translational Repression of IL-10 and Cyclooxygenase-2 J. Immunol., 2018-04-30;0(0):. 4/30/2018 [PMID: 29712774]
• S Dyck, H Kataria, A Alizadeh, KT Santhosh, B Lang, J Silver, S Karimi-Abd Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTP? receptors promotes a beneficial inflammatory response following spinal cord injury J Neuroinflammation, 2018-03-20;15(1):90. 2018-03-20 [PMID: 29558941] (IHC, Neutralization, Rat)
• JA Varner, P Foubert, MM Kaneda PI3Kgamma activates integrin alpha4 and promotes immune suppressive myeloid cell polarization during tumor progression Cancer Immunol Res, 2017-09-28;0(0):. 2017-09-28 [PMID: 28963139] (Neutralization, Mouse)
• LX Li, JC Labuda, DM Imai, SM Griffey, SJ McSorley CCR7 Deficiency Allows Accelerated Clearance of Chlamydia from the Female Reproductive Tract J. Immunol., 2017-08-11;0(0):. 2017-08-11 [PMID: 28801359] (ELISA Development (Capture), Mouse)
• Ya-Jun Ren, Lei Zhang, Tao Bai, Hong-Lu Yu, Ying Li, Wei Qian et al. Transfer of CD11c+ lamina propria mononuclear phagocytes from post-infectious irritable bowel syndrome causes mucosal barrier dysfunction and visceral hypersensitivity in recipient mice International Journal of Molecular Medicine 6/1/2017 [PMID: 28440501]
• DC Nascimento, PH Melo, AR Piñeros, RG Ferreira, DF Colón, PB Donate, FV Castanheir, A Gozzi, PG Czaikoski, W Niedbala, MC Borges, DS Zamboni, FY Liew, FQ Cunha, JC Alves-Filh IL-33 contributes to sepsis-induced long-term immunosuppression by expanding the regulatory T cell population Nat Commun, 2017-04-04;8(0):14919. 2017-04-04 [PMID: 28374774] (Functional Assay, Mouse)
• W Dawicki, C Li, J Town, X Zhang, JR Gordon Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3(+)CD49b(-)Foxp3(-) regulatory T�cells J. Allergy Clin. Immunol., 2016-10-26;139(5):1608-1620.e3. 2016-10-26 [PMID: 28277274] (ELISA Development (Capture), Mouse)
• Andrew Leber, Josep Bassaganya-Riera, Nuria Tubau-Juni, Victoria Zoccoli-Rodriguez, Pinyi Lu, Victoria Godfrey et al. Lanthionine Synthetase C-Like 2 Modulates Immune Responses to Influenza Virus Infection Frontiers in Immunology 2/21/2017 [PMID: 28270815]
• AJ Byrne, M Weiss, SA Mathie, SA Walker, HL Eames, D Saliba, CM Lloyd, IA Udalova A critical role for IRF5 in regulating allergic airway inflammation Mucosal Immunol, 2016-10-19;10(3):716-726. 2016-10-19 [PMID: 27759022] (ELISA Development (Capture), Mouse)
• Yaoqin Hu Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response Mediators Inflamm, 2016-07-27;2016(0):3735452. 2016-07-27 [PMID: 27546994] (Neutralization, Mouse)
• Jung-Yeon Lim Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis Sci Rep, 2016-06-01;6(0):26851. 2016-06-01 [PMID: 27246365] (ELISA Development (Capture), Mouse)
• Ting Liu, Jun Ren, Wei Wang, Xia-wei Wei, Guo-bo Shen, Yan-tong Liu et al. Treatment of dextran sodium sulfate-induced experimental colitis by adoptive transfer of peritoneal cells Scientific Reports 11/13/2015 [PMID: 26565726]
• Yang Jiang, Qi Gao, Liyang Wang, Chun Guo, Faliang Zhu, Bo Wang et al. Deficiency of programmed cell death 4 results in increased IL-10 expression by macrophages and thereby attenuates atherosclerosis in hyperlipidemic mice Cellular & Molecular Immunology 7/1/2016 [PMID: 26166769]
• Besnard A, Guabiraba R, Niedbala W, Palomo J, Reverchon F, Shaw T, Couper K, Ryffel B, Liew F IL-33-mediated protection against experimental cerebral malaria is linked to induction of type 2 innate lymphoid cells, M2 macrophages and regulatory T cells. PLoS Pathog, 2015-02-06;11(2):e1004607. 2015-02-06 [PMID: 25659095] (Mouse)
• McKelvey R, Berta T, Old E, Ji R, Fitzgerald M Neuropathic pain is constitutively suppressed in early life by anti-inflammatory neuroimmune regulation. J Neurosci, 2015-01-14;35(2):457-66. 2015-01-14 [PMID: 25589741] (Neutralization, Mouse)
• Ohama H, Asai A, Ito I, Suzuki S, Kobayashi M, Higuchi K, Suzuki F M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections. Am J Pathol, 2014-12-06;185(2):420-31. 2014-12-06 [PMID: 25485859] (Functional Assay, Mouse)
• Lalani A, Moore C, Luo C, Kreider B, Liu Y, Morse H, Xie P Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice. J Immunol, 2014-11-24;194(1):334-48. 2014-11-24 [PMID: 25422508] (ELISA Development, Mouse)
• Hsu , Kenneth, Chung , Yuen Min, Endoh , Yasumi, Geczy , Carolyn TLR9 ligands induce S100A8 in macrophages via a STAT3-dependent pathway which requires IL-10 and PGE2. PLoS ONE, 2014-08-06;9(8):e103629. 2014-08-06 [PMID: 25098409] (Neutralization, Mouse)
• Kothari P, Pestana R, Mesraoua R, Elchaki R, Khan K, Dannenberg A, Falcone D IL-6-mediated induction of matrix metalloproteinase-9 is modulated by JAK-dependent IL-10 expression in macrophages. J Immunol, 2013-11-27;192(1):349-57. 2013-11-27 [PMID: 24285838] (Bioassay, Mouse)
• Kimura T, Sugaya M, Blauvelt A, Okochi H, Sato S Delayed wound healing due to increased interleukin-10 expression in mice with lymphatic dysfunction. J Leukoc Biol, 2013-05-06;94(1):137-45. 2013-05-06 [PMID: 23650621] (IHC, Mouse)
• Wang YC, Sung WW, Wu TC, Wang L, Chien WP, Cheng YW, Chen CY, Shieh SH, Lee H Interleukin-10 haplotype may predict survival and relapse in resected non-small cell lung cancer. PLoS ONE, 2012-07-27;7(7):e39525. 2012-07-27 [PMID: 22848356] (Neutralization, Mouse)
• Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse)
• Aline Bolpetti, João S Silva, Luisa L Villa, Ana Paula Lepique Interleukin-10 production by tumor infiltrating macrophages plays a role in Human Papillomavirus 16 tumor growth BMC Immunology 12/1/2010 [PMID: 20525400]
• Lu H, Wagner WM, Gad E, Yang Y, Duan H, Amon LM, Van Denend N, Larson ER, Chang A, Tufvesson H, Disis ML Treatment failure of a TLR-7 agonist occurs due to self-regulation of acute inflammation and can be overcome by IL-10 blockade. J. Immunol., 2010-03-22;184(9):5360-7. 2010-03-22 [PMID: 20308630] (Neutralization, Mouse)
• M. Nedim Ince, David E. Elliott, Tommy Setiawan, Ahmed Metwali, Arthur Blum, Hung-lin Chen et al. Role of T cell TGF-beta signaling in intestinal cytokine responses and helminthic immune modulation European Journal of Immunology 6/2/2010 [PMID: 19544487]
• Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis. Microbes Infect., 2009-03-13;11(4):524-30. 2009-03-13 [PMID: 19286469] (ELISA Development, Mouse)
• Ye F, Yan S, Xu L, Jiang Z, Liu N, Xiong S, Wang Y, Chu Y Tr1 regulatory T cells induced by ConA pretreatment prevent mice from ConA-induced hepatitis. Immunol. Lett., 2009-02-04;122(2):198-207. 2009-02-04 [PMID: 19200442] (Neutralization, Mouse)
• Meiron M, Zohar Y, Anunu R, Wildbaum G, Karin N CXCL12 (SDF-1alpha) suppresses ongoing experimental autoimmune encephalomyelitis by selecting antigen-specific regulatory T cells. J. Exp. Med., 2008-10-13;205(11):2643-55. 2008-10-13 [PMID: 18852294] (Functional Assay, IHC-P, Neutralization, Mouse)
• Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis. Immunology, 2008-04-03;125(2):272-9. 2008-04-03 [PMID: 18393969] (ELISA Development, Mouse)
• Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP Tumor-specific Th17-polarized cells eradicate large established melanoma. Blood, 2008-03-19;112(2):362-73. 2008-03-19 [PMID: 18354038] (ELISA Development, Mouse)
• Ismail N, Crossley EC, Stevenson HL, Walker DH Relative importance of T-cell subsets in monocytotropic ehrlichiosis: a novel effector mechanism involved in Ehrlichia-induced immunopathology in murine ehrlichiosis. Infect. Immun., 2007-06-11;75(9):4608-20. 2007-06-11 [PMID: 17562770] (Neutralization, Mouse)
• Hamdi H, Godot V, Maillot MC, Prejean MV, Cohen N, Krzysiek R, Lemoine FM, Zou W, Emilie D Induction of antigen-specific regulatory T lymphocytes by human dendritic cells expressing the glucocorticoid-induced leucine zipper. Blood, 2007-03-13;110(1):211-9. 2007-03-13 [PMID: 17356131] (Neutralization, Mouse)
• Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors. Infect. Immun., 2006-09-18;74(12):6730-8. 2006-09-18 [PMID: 16982824] (ELISA Development, Human)
• Osuchowski MF, Welch K, Siddiqui J, Remick DG Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality. J. Immunol., 2006-08-01;177(3):1967-74. 2006-08-01 [PMID: 16849510] (ELISA Development, Mouse)
• Wen H, Hogaboam CM, Gauldie J, Kunkel SL Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile. Am. J. Pathol., 2006-06-01;168(6):1940-50. 2006-06-01 [PMID: 16723709] (ELISA Development, Mouse)
• Osuchowski MF, Siddiqui J, Copeland S, Remick DG Sequential ELISA to profile multiple cytokines from small volumes. J. Immunol. Methods, 2005-07-01;302(1):172-81. 2005-07-01 [PMID: 16023134] (ELISA Development, Mouse)
• Ellaban E, Bolgos G, Remick D Selective macrophage suppression during sepsis. Cell. Immunol., 2005-02-26;231(1):103-11. 2005-02-26 [PMID: 15919375] (ELISA Development, Mouse)
• Rosini L, Matlack R, Taylor J, Howell KF, Yeh K, Pennello A, Riggs JE Nonlymphoid peritoneal cells suppress the T cell response to Mls. Immunobiology, 2004-01-01;209(8):575-84. 2004-01-01 [PMID: 15638126] (Neutralization, Mouse)
• Bednarski JJ, Warner RE, Rao T Attenuation of autoimmune disease in Fas-deficient mice by treatment with a cytotoxic benzodiazepine. Arthritis Rheum., 2003-03-01;48(3):757-66. 2003-03-01 [PMID: 12632430] (ELISpot Development, Mouse)

Bioinformatics

• Gene Symbol: IL10
• Uniprot:
  • P18893()