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IL-10 Antibody (997935) [Unconjugated]

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IL‑10 was detected in immersion fixed paraffin-embedded sections of human tonil using Rat Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB92101) at 0.5 µg/mL for 1 hour at room temperature followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
997935
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated

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Catalog# & Formulation Size Price

IL-10 Antibody (997935) [Unconjugated] Summary

Immunogen
Human embryonic kidney cell, HEK293-derived human IL-10
Met1-Asn178
Accession # P22301
Specificity
Detects human IL-10 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Purity Statement
Protein A or G purified from cell culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 0.5-25 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB92101 in the following application:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10 Antibody (997935) [Unconjugated]

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • GVHDS
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF

Background

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19,
IL‑20, IL-22, IL-24, and IL-26/AK155 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2‑5). Mature human IL-10 shares 72%‑86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (6, 8, 9). The IL-10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (10). IL-10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL‑20 R alpha , IL-22 R alpha , or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL‑29
(11‑13). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (14‑16). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

  1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  2. O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425.
  3. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
  4. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
  5. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
  6. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
  7. Hsu, D.-H. et al. (1990) Science 250:830.
  8. Windsor, W.T. et al. (1993) Biochemistry 32:8807.
  9. Syto, R. et al. (1998) Biochemistry 37:16943.
  10. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  11. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  12. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
  13. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  14. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
  15. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
  16. Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-10 Antibody (MAB92101) (0)

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Review for IL-10 Antibody (MAB92101) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB92101:
Filter by Applications
IHC-Fr
(1)
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(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen IL-10 MAB92101
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5
reviewed by:
Tyler Crowe
IHC-Fr Human 12/14/2018
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFrozen human skin sections
SpeciesHuman
LotCLVE011812A

Comments

CommentsSections fixed, permeablized, blocked with 5% BSA, and stained with antibody diluted 1:200 in blocking solution

FAQs for IL-10 Antibody (MAB92101) (0)

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Tyler Crowe
12/14/2018
Application: IHC-Fr
Species: Human

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