IL-10 Antibody (262715) [Unconjugated]

IL-10 was detected in immersion fixed porcine peripheral blood mononuclear cells treated with calcium ionomycin and PMA using Mouse Anti-Porcine IL-10 Monoclonal Antibody (Catalog # MAB6932) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Po
• Applications: ICC/IF
• Clone: 262715
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-10 Antibody (262715) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant porcine IL-10
  Ser19-Asn175
  Accession # Q29055
• Specificity: Detects porcine IL-10 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant canine IL-10, recombinant feline IL-10, recombinant human IL-10, and recombinant viral IL-10 is observed and no cross-reactivity with recombinant cotton rat IL-10, recombinant equine IL-10, recombinant mouse IL-10, recombinant rat IL-10, or recombinant human IL-22 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL10
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10 Antibody (262715) [Unconjugated]

• CSIF
• CSIFMGC126450
• Cytokine synthesis inhibitory factor
• GVHDS
• IL10
• IL-10
• IL10A
• IL-10MGC126451
• interleukin 10
• interleukin-10
• TGIF

Background

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (Th2) clones that inhibited the cytokine production by Th1 clones which are dependent upon stimulation with antigen in the presence of antigen presenting cells (APC). Murine IL-10 is produced by Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, and LY-1⁺ (CD5⁺) and normal B cells. Human IL-10 has cross-species actvities and is active on mouse cells. Murine IL-10 is species-specific and does not act on human cells. Porcine IL-10 shares 71% and 78% amino acid sequence identity with mouse and human
IL-10, respectively. IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is a potent immunosuppressant of macrophage functions. In vitro, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by, among other effects, down‑regulating class II MHC expression. Thus, IL-10 can inhibit monocyte/macrophage-dependent, antigen stimulated cytokine synthesis (especially IFN-gamma ) by human PBMNC and NK, and mouse Th1 cells. Additionally, IL-10 is a potent inhibitor of monocyte/macrophage activation and its resultant cytotoxic effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation, Ig secretion, and class II MHC expression. Aside from B lymphocytes, IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development.

1. Moore, K.W. et al. (1993) Annu. Rev. Immunol. 11:165.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-10 Antibody (MAB6932)(2)

Publications using MAB6932

• Jiwakanon Jatesada, Persson Elisabeth, Dalin Anne-Marie Seminal plasma did not influence the presence of transforming growth factor-beta 1, interleukine-10 and interleukin-6 in porcine follicles shortly after insemination Acta Veterinaria Scandinavica 12/1/2013 [PMID: 24020676]
• Nadworny PL, Wang J, Tredget EE Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis. J Inflamm (Lond), 2010-02-19;7(0):13. 2010-02-19 [PMID: 20170497] (IHC-P, Porcine)

Review for IL-10 Antibody (MAB6932) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer Flow Other 02/25/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 23359497
• Species: Other

Bioinformatics

• Gene Symbol: IL10
• Uniprot:
  • Q29055()