Novus Biologicals products are now on

IL-10 Antibody (25209) [Unconjugated]


Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL-10 Monoclonal more
Recombinant Human IL‑10 (Catalog # 217-IL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑10 (5 ng/mL) is more
S100A8 and S100A9 mRNA expression increases were partially abrogated by IL-10 blockade.Messenger RNA (mRNA) level of TNF alpha , IL-10, S100A8 and S100A9 in an ex vivo model of endotoxin tolerance in the presence or more
S100A8 and S100A9 mRNA expression increases were partially abrogated by IL-10 blockade.Messenger RNA (mRNA) level of TNF alpha , IL-10, S100A8 and S100A9 in an ex vivo model of endotoxin tolerance in the presence or more
S100A8 and S100A9 mRNA expression increases were partially abrogated by IL-10 blockade.Messenger RNA (mRNA) level of TNF alpha , IL-10, S100A8 and S100A9 in an ex vivo model of endotoxin tolerance in the presence or more
S100A8 and S100A9 mRNA expression increases were partially abrogated by IL-10 blockade.Messenger RNA (mRNA) level of TNF alpha , IL-10, S100A8 and S100A9 in an ex vivo model of endotoxin tolerance in the presence or more

Product Details

Reactivity HuSpecies Glossary
Applications WB, B/N

Order Details

View Available Formulations
Catalog# & Formulation Size Price

IL-10 Antibody (25209) [Unconjugated] Summary

E. coli-derived recombinant human IL-10
Accession # P22301
Detects human IL-10 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse IL‑10.
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Neutralization 0.005-0.03 ug/mL
  • Western Blot 5 ug/mL
Read Publications using
MAB2171 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10 Antibody (25209) [Unconjugated]

  • CSIF
  • CSIFMGC126450
  • Cytokine synthesis inhibitory factor
  • IL10
  • IL-10
  • IL10A
  • IL-10MGC126451
  • interleukin 10
  • interleukin-10
  • TGIF


IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Mu
Applications: ELISA
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Species: Mu
Applications: ELISA
Species: Hu
Applications: BA
Species: Mu
Applications: ELISA
Species: Hu
Applications: BA
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
Species: Bv, Hu, Mu, Rt
Applications: Dual ISH-IHC, Flow, IB, ICC/IF, IHC, IHC-P, IP, Single-Cell Western, WB
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KO, WB
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, Neut
Species: Hu
Applications: ELISA

Publications for IL-10 Antibody (MAB2171)(17)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Bioassay, Flow Cytometry, Neutralization.

Filter By Application
Flow Cytometry
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using MAB2171 Applications Species
von Glehn, F;Pochet, N;Thapa, B;Raheja, R;Mazzola, MA;Jangi, S;Beynon, V;Huang, J;Farias, AS;Paul, A;Santos, LMB;Gandhi, R;Murugaiyan, G;Weiner, HL;Baecher-Allan, CM; Defective Induction of IL-27-Mediated Immunoregulation by Myeloid DCs in Multiple Sclerosis International journal of molecular sciences 2023-04-28 [PMID: 37175706] (Neutralization, Human) Neutralization Human
MR Shokri, M Bozorgmehr, A Ghanavatin, R Falak, M Aleahmad, S Kazemnejad, F Shokri, AH Zarnani Human menstrual blood-derived stromal/stem cells modulate functional features of natural killer cells Sci Rep, 2019-07-10;9(1):10007. 2019-07-10 [PMID: 31292483] (Bioassay, Human) Bioassay Human
Hongxiao Song, Guangyun Tan, Yang Yang, An Cui, Haijun Li, Tianyang Li et al. Hepatitis B Virus–Induced Imbalance of Inflammatory and Antiviral Signaling by Differential Phosphorylation of STAT1 in Human Monocytes The Journal of Immunology 2019-04-15 [PMID: 30842274]
R Tzach-Nahm, R Nashef, O Fleissig, A Palmon, L Shapira, A Wilensky, G Nussbaum Oral fibroblasts modulate the macrophage response to bacterial challenge Sci Rep, 2017-09-14;7(1):11516. 2017-09-14 [PMID: 28912533] (Neutralization, Human) Neutralization Human
S Nakachi, S Sumitomo, Y Tsuchida, H Tsuchiya, M Kono, R Kato, K Sakurai, N Hanata, Y Nagafuchi, S Tateishi, H Kanda, T Okamura, K Yamamoto, K Fujio Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis Arthritis Res. Ther., 2017-05-16;19(1):97. 2017-05-16 [PMID: 28511719] (Neutralization, Human) Neutralization Human
Fontaine M, Planel S, Peronnet E, Turrel-Davin F, Piriou V, Pachot A, Monneret G, Lepape A, Venet F S100A8/A9 mRNA induction in an ex vivo model of endotoxin tolerance: roles of IL-10 and IFNgamma. PLoS ONE, 2014-06-23;9(6):e100909. 2014-06-23 [PMID: 24956170] (Neutralization, Human) Neutralization Human
Planes R, BenMohamed L, Leghmari K, Delobel P, Izopet J, Bahraoui E HIV-1 Tat protein induces PD-L1 (B7-H1) expression on dendritic cells through tumor necrosis factor alpha- and toll-like receptor 4-mediated mechanisms. J Virol, 2014-04-02;88(12):6672-89. 2014-04-02 [PMID: 24696476] (Neutralization, Human) Neutralization Human
Svajger U, Obermajer N, Jeras M IFN-gamma-rich environment programs dendritic cells toward silencing of cytotoxic immune responses. J Leukoc Biol, 2013-08-07;95(1):33-46. 2013-08-07 [PMID: 23924658] (Neutralization, Human) Neutralization Human
Wang YC, Sung WW, Wu TC, Wang L, Chien WP, Cheng YW, Chen CY, Shieh SH, Lee H Interleukin-10 haplotype may predict survival and relapse in resected non-small cell lung cancer. PLoS ONE, 2012-07-27;7(7):e39525. 7/27/2012 [PMID: 22848356]
Nicolaidou V, Wong MM, Redpath AN Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation. PLoS ONE, 2012-07-03;7(7):e39871. 2012-07-03 [PMID: 22802946] (Neutralization, Human) Neutralization Human
Show All 17 Publications.

Reviews for IL-10 Antibody (MAB2171) (0)

There are no reviews for IL-10 Antibody (MAB2171). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-10 Antibody (MAB2171) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional IL-10 Products

Blogs on IL-10.

  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

How To Identify B Cell Subsets Using Flow Cytometry
By Victoria OsinskiUsing Flow Cytometry to Identify B Cell SubsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified...  Read full blog post.

PPAR gamma - An important target in human metabolism
Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells by interacting with members of the nuclear hormone receptor superfamily known as peroxisome proliferator activated receptors (PPARs). There are f...  Read full blog post.

CD11b: Marker for a New Type of B Cell that Participates in Cell-Mediated Immunity
Think B lymphocytes just produce antibodies? Think again! Although, of course, B cells are vital for the humoral immune response, many studies in recent years have begun to uncover antibody-independent actions of B cells: regulating T cells and thus a...  Read full blog post.

mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our IL-10 Antibody (25209) [Unconjugated] and receive a gift card or discount.


Gene Symbol IL10