IL-10 Antibody (25209) [Unconjugated]

Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2171) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Recombinant Human IL‑10 (Catalog # 217-IL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑10 Monoclonal Antibody (Catalog # MAB2171). The ND50 is typically 0.005‑0.03 µg/mL.

S100A8 and S100A9 mRNA expression increases were partially abrogated by IL-10 blockade.Messenger RNA (mRNA) level of TNF alpha , IL-10, S100A8 and S100A9 in an ex vivo model of endotoxin tolerance in the presence or absence of anti-IL-10 antibodies (Ab). Anti IL-10 Ab was used at 100 ng/ml. The mRNA level was normalized to that of the reference gene peptidylpropylisomerase B (PPIB) and then compared to the control group. Black columns represent controls (cells without any lipopolysaccharide (LPS)), white columns represent LPS-unprimed cells (only stimulated once with 100 ng/ml LPS) and grey columns represent LPS-primed cells (stimulated twice: 2 ng/ml followed by 100 ng/ml). †p<0.01, Wilcoxon paired test. Median (+/− interquartile range) data from 9 independent experiments are given. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24956170), licensed under a CC-BY license. Not internally tested by R&D Systems.

S100A8 and S100A9 mRNA expression increases were partially abrogated by IL-10 blockade.Messenger RNA (mRNA) level of TNF alpha , IL-10, S100A8 and S100A9 in an ex vivo model of endotoxin tolerance in the presence or absence of anti-IL-10 antibodies (Ab). Anti IL-10 Ab was used at 100 ng/ml. The mRNA level was normalized to that of the reference gene peptidylpropylisomerase B (PPIB) and then compared to the control group. Black columns represent controls (cells without any lipopolysaccharide (LPS)), white columns represent LPS-unprimed cells (only stimulated once with 100 ng/ml LPS) and grey columns represent LPS-primed cells (stimulated twice: 2 ng/ml followed by 100 ng/ml). †p<0.01, Wilcoxon paired test. Median (+/− interquartile range) data from 9 independent experiments are given. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24956170), licensed under a CC-BY license. Not internally tested by R&D Systems.

S100A8 and S100A9 mRNA expression increases were partially abrogated by IL-10 blockade.Messenger RNA (mRNA) level of TNF alpha , IL-10, S100A8 and S100A9 in an ex vivo model of endotoxin tolerance in the presence or absence of anti-IL-10 antibodies (Ab). Anti IL-10 Ab was used at 100 ng/ml. The mRNA level was normalized to that of the reference gene peptidylpropylisomerase B (PPIB) and then compared to the control group. Black columns represent controls (cells without any lipopolysaccharide (LPS)), white columns represent LPS-unprimed cells (only stimulated once with 100 ng/ml LPS) and grey columns represent LPS-primed cells (stimulated twice: 2 ng/ml followed by 100 ng/ml). †p<0.01, Wilcoxon paired test. Median (+/− interquartile range) data from 9 independent experiments are given. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24956170), licensed under a CC-BY license. Not internally tested by R&D Systems.

S100A8 and S100A9 mRNA expression increases were partially abrogated by IL-10 blockade.Messenger RNA (mRNA) level of TNF alpha , IL-10, S100A8 and S100A9 in an ex vivo model of endotoxin tolerance in the presence or absence of anti-IL-10 antibodies (Ab). Anti IL-10 Ab was used at 100 ng/ml. The mRNA level was normalized to that of the reference gene peptidylpropylisomerase B (PPIB) and then compared to the control group. Black columns represent controls (cells without any lipopolysaccharide (LPS)), white columns represent LPS-unprimed cells (only stimulated once with 100 ng/ml LPS) and grey columns represent LPS-primed cells (stimulated twice: 2 ng/ml followed by 100 ng/ml). †p<0.01, Wilcoxon paired test. Median (+/− interquartile range) data from 9 independent experiments are given. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24956170), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, B/N
• Clone: 25209
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-10 Antibody (25209) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IL-10
  Ser19-Asn178
  Accession # P22301
• Specificity: Detects human IL-10 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse IL‑10.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL10
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.005-0.03 ug/mL
  • Western Blot 5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-10 Antibody (25209) [Unconjugated]

• CSIF
• CSIFMGC126450
• Cytokine synthesis inhibitory factor
• GVHDS
• IL10
• IL-10
• IL10A
• IL-10MGC126451
• interleukin 10
• interleukin-10
• TGIF

Background

IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-10 Antibody (MAB2171)(17)

Publications using MAB2171

• von Glehn, F;Pochet, N;Thapa, B;Raheja, R;Mazzola, MA;Jangi, S;Beynon, V;Huang, J;Farias, AS;Paul, A;Santos, LMB;Gandhi, R;Murugaiyan, G;Weiner, HL;Baecher-Allan, CM; Defective Induction of IL-27-Mediated Immunoregulation by Myeloid DCs in Multiple Sclerosis International journal of molecular sciences 2023-04-28 [PMID: 37175706] (Neutralization, Human)
• MR Shokri, M Bozorgmehr, A Ghanavatin, R Falak, M Aleahmad, S Kazemnejad, F Shokri, AH Zarnani Human menstrual blood-derived stromal/stem cells modulate functional features of natural killer cells Sci Rep, 2019-07-10;9(1):10007. 2019-07-10 [PMID: 31292483] (Bioassay, Human)
• Hongxiao Song, Guangyun Tan, Yang Yang, An Cui, Haijun Li, Tianyang Li et al. Hepatitis B Virus–Induced Imbalance of Inflammatory and Antiviral Signaling by Differential Phosphorylation of STAT1 in Human Monocytes The Journal of Immunology 2019-04-15 [PMID: 30842274]
• R Tzach-Nahm, R Nashef, O Fleissig, A Palmon, L Shapira, A Wilensky, G Nussbaum Oral fibroblasts modulate the macrophage response to bacterial challenge Sci Rep, 2017-09-14;7(1):11516. 2017-09-14 [PMID: 28912533] (Neutralization, Human)
• S Nakachi, S Sumitomo, Y Tsuchida, H Tsuchiya, M Kono, R Kato, K Sakurai, N Hanata, Y Nagafuchi, S Tateishi, H Kanda, T Okamura, K Yamamoto, K Fujio Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis Arthritis Res. Ther., 2017-05-16;19(1):97. 2017-05-16 [PMID: 28511719] (Neutralization, Human)
• Fontaine M, Planel S, Peronnet E, Turrel-Davin F, Piriou V, Pachot A, Monneret G, Lepape A, Venet F S100A8/A9 mRNA induction in an ex vivo model of endotoxin tolerance: roles of IL-10 and IFNgamma. PLoS ONE, 2014-06-23;9(6):e100909. 2014-06-23 [PMID: 24956170] (Neutralization, Human)
• Planes R, BenMohamed L, Leghmari K, Delobel P, Izopet J, Bahraoui E HIV-1 Tat protein induces PD-L1 (B7-H1) expression on dendritic cells through tumor necrosis factor alpha- and toll-like receptor 4-mediated mechanisms. J Virol, 2014-04-02;88(12):6672-89. 2014-04-02 [PMID: 24696476] (Neutralization, Human)
• Svajger U, Obermajer N, Jeras M IFN-gamma-rich environment programs dendritic cells toward silencing of cytotoxic immune responses. J Leukoc Biol, 2013-08-07;95(1):33-46. 2013-08-07 [PMID: 23924658] (Neutralization, Human)
• Wang YC, Sung WW, Wu TC, Wang L, Chien WP, Cheng YW, Chen CY, Shieh SH, Lee H Interleukin-10 haplotype may predict survival and relapse in resected non-small cell lung cancer. PLoS ONE, 2012-07-27;7(7):e39525. 7/27/2012 [PMID: 22848356]
• Nicolaidou V, Wong MM, Redpath AN Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation. PLoS ONE, 2012-07-03;7(7):e39871. 2012-07-03 [PMID: 22802946] (Neutralization, Human)
• Svajger U, Obermajer N, Anderluh M, Kos J, Jeras M DC-SIGN ligation greatly affects dendritic cell differentiation from monocytes compromising their normal function. J. Leukoc. Biol., 2011-03-29;89(6):893-905. 2011-03-29 [PMID: 21447648] (Neutralization, Human)
• Lina A. Chusid, Lucy Pereira-Argenziano, Veronika Miskolci, Ivana Vancurova, Dennis Davidson Transcriptional Control of Cytokine Release from Monocytes of the Newborn: Effects of Endogenous and Exogenous Interleukin-10 versus Dexamethasone Neonatology 2010-01-01 [PMID: 19713717] (Neutralization, Human)
• Tran DQ, Glass DD, Uzel G, Darnell DA, Spalding C, Holland SM, Shevach EM Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function. J. Immunol., 2009-03-01;182(5):2929-38. 2009-03-01 [PMID: 19234188] (Neutralization, Mouse)
• Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. Eur. J. Immunol., 2008-04-01;38(4):1012-23. 2008-04-01 [PMID: 18350544] (Flow Cytometry, Human)
• Hajishengallis G, Shakhatreh MA, Wang M, Liang S Complement receptor 3 blockade promotes IL-12-Mediated Clearance of Porphyromonas gingivalis and Negates Its Virulence In Vivo. J. Immunol., 2007-08-15;179(4):2359-67. 2007-08-15 [PMID: 17675497] (Neutralization, Human)
• Susanne Nylén, Radheshyam Maurya, Liv Eidsmo, Krishna Das Manandhar, Shyam Sundar, David Sacks Splenic accumulation of IL-10 mRNA in T cells distinct from CD4+CD25+ (Foxp3) regulatory T cells in human visceral leishmaniasis The Journal of Experimental Medicine 2007-04-16 [PMID: 17389235]
• Perez S, Mahaira L, Sotiropoulou P, Gritzapis A, Iliopoulou E, Niarchos D, Cacoullos N, Kavalakis Y, Antsaklis A, Sotiriadou N, Baxevanis C, Papamichail M Effect of IL-21 on NK cells derived from different umbilical cord blood populations. Int Immunol, 2005-11-15;18(1):49-58. 2005-11-15 [PMID: 16291655] (Neutralization, Human)

Bioinformatics

• Gene Symbol: IL10
• Uniprot:
  • P22301()