IL-1 beta/IL-1F2 Antibody [Biotin]

IL‑1 beta /IL‑1F2 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line using Goat Anti-Human IL‑1 beta /IL‑1F2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF201) at 5 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of THP-1 cells treated with 200nM PMA for 24 hours then 10ug/mL LPS for 24 hours. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL-1 beta /IL-1F2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL-1 beta /IL-1F2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Differences in Functional Profiles between Three Patients with Different Clinical Outcomesgp100 209–2M spots co-spotted with anti-IFN gamma , anti-TNF alpha , anti-IL1b, and anti-IL-6 are shown for three separate patient samples. Patient 10735, who remains free of disease at this writing, displays strong IFN gamma , TNF alpha , IL-1b, and IL-6 secretion. Patients 10710 and 10737 show strong IFN gamma secretion, but weak to no TNF alpha , IL-1b, and IL-6 secretion; both patients experienced disease recurrence soon after these samples were drawn. Note the diffuse pattern of IL-1b and IL-6 capture, which differs from the focal capture of IFN gamma and TNF alpha . Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/16162034), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, ELISA(Det), ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-1 beta/IL-1F2 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human IL-1 beta /IL-1F2
  Ala117-Ser269
  Accession # NP_000567
• Specificity: Detects human IL-1 beta /IL-1F2 in ELISAs and Western blots. In sandwich immunoassays, less than 4% cross-reactivity with recombinant rat
  IL‑1 beta is observed and less than 0.1% cross-reactivity with recombinant mouse IL‑1 beta and recombinant porcine IL‑1 beta is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL1B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human IL-1 beta/IL-1F2 Antibody (Catalog # MAB601)
  ELISA Detection: Human IL-1 beta/IL-1F2 Biotinylated Antibody (Catalog # BAF201)
  Standard: Recombinant Human IL-1 beta/IL-1F2 (Catalog # 201-LB)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-1 beta/IL-1F2 Antibody [Biotin]

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta . IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-1 beta/IL-1F2 Antibody (BAF201)(30)

Publications using BAF201

• An, J;Kim, BS;Yoon, HJ; Combination of gut microbiota, proinflammatory cytokine, and 18F-FDG PET as potential indicators for predicting breast cancer recurrence Scientific reports 2025-03-10 [PMID: 40065046] (ELISA Detection, Human)
• Marina Chan, Siddharth Vijay, John McNevin, M Juliana McElrath, Eric C Holland, Taranjit S Gujral Machine learning identifies molecular regulators and therapeutics for targeting SARS‐CoV2‐induced cytokine release Molecular Systems Biology 9/6/2021 [PMID: 34486798]
• Lai J, Wu D, Wu C et al. Mitochondrial CMPK2 mediates immunomodulatory and antiviral activities through IFN-dependent and IFN-independent pathways iScience 6/18/2021 [PMID: 34142025]
• Nikki P. L. Toledo, Hongzhao Li, Robert W. Omange, Tamara G. Dacoba, Jose Crecente-Campo, Dane Schalk et al. Cervico-Vaginal Inflammatory Cytokine and Chemokine Responses to Two Different SIV Immunogens Frontiers in Immunology 8/25/2020 [PMID: 32983121]
• Mehdi Bouhaddou, Danish Memon, Bjoern Meyer, Kris M. White, Veronica V. Rezelj, Miguel Correa Marrero et al. The Global Phosphorylation Landscape of SARS-CoV-2 Infection Cell 8/1/2020 [PMID: 32645325]
• Larisa I Labzin, Maria Bottermann, Pablo Rodriguez‐Silvestre, Stian Foss, Jan Terje Andersen, Marina Vaysburd et al. Antibody and DNA sensing pathways converge to activate the inflammasome during primary human macrophage infection The EMBO Journal 11/4/2019 [PMID: 31468569]
• Sandra Sacre, Albert Jaxa-Chamiec, Caroline M. R. Low, Giselle Chamberlain, Cathy Tralau-Stewart Structural Modification of the Antidepressant Mianserin Suggests That Its Anti-inflammatory Activity May Be Independent of 5-Hydroxytryptamine Receptors Frontiers in Immunology 5/24/2019 [PMID: 31244825]
• Tomas Castro-Dopico, Thomas W. Dennison, John R. Ferdinand, Rebeccah J. Mathews, Aaron Fleming, Dean Clift et al. Anti-commensal IgG Drives Intestinal Inflammation and Type 17 Immunity in Ulcerative Colitis Immunity 4/16/2019 [PMID: 30876876]
• Y Ninomiya, SI Tanuma, M Tsukimoto Differences in the effects of four TRPV1 channel antagonists on lipopolysaccharide-induced cytokine production and COX-2 expression in murine macrophages Biochem. Biophys. Res. Commun, 2017-01-31;0(0):. 2017-01-31 [PMID: 28153725] (Western Blot, Mouse)
• Agnetha Folestad, Martin Ålund, Susanne Asteberg, Jesper Fowelin, Ylva Aurell, Jan Göthlin et al. Offloading treatment is linked to activation of proinflammatory cytokines and start of bone repair and remodeling in Charcot arthropathy patients Journal of Foot and Ankle Research 12/10/2015 [PMID: 26692902]
• Manfè Valentina, Fleckner Jan, Nørby Lisby Peder, Zhang Bo, Dai Hongjie, Keller Pernille Cytokine detection and simultaneous assessment of rheumatoid factor interference in human serum and synovial fluid using high-sensitivity protein arrays on plasmonic gold chips BMC Biotechnology 12/1/2015 [PMID: 26268325]
• Stoppelkamp S, Veseli K, Stang K, Schlensak C, Wendel H, Walker T Identification of Predictive Early Biomarkers for Sterile-SIRS after Cardiovascular Surgery. PLoS ONE, 2015-08-11;10(8):e0135527. 8/11/2015 [PMID: 26263001]
• Duong B, Onizawa M, Oses-Prieto J, Advincula R, Burlingame A, Malynn B, Ma A A20 restricts ubiquitination of pro-interleukin-1beta protein complexes and suppresses NLRP3 inflammasome activity. Immunity, 2015-01-20;42(1):55-67. 2015-01-20 [PMID: 25607459] (Western Blot, Mouse)
• Hong YS, Moon SJ, Moon S, Joo YB, Joo Y, Jeon CH, Jeon C, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis. J Korean Med Sci, 2011-09-01;26(9):1132-9. 2011-09-01 [PMID: 21935266] (ELISA Development, Human)
• Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin. J. Immunol., 2009-10-07;183(9):5799-806. 2009-10-07 [PMID: 19812208] (ELISA Development, Human)
• Hu H, Kwun J, Aizenstein BD, Knechtle SJ Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation, 2009-06-27;87(12):1814-20. 2009-06-27 [PMID: 19543058] (Antibody Array Development, Human)
• Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA Endogenous IL-32 controls cytokine and HIV-1 production. J. Immunol., 2008-07-01;181(1):557-65. 2008-07-01 [PMID: 18566422] (Electrochemiluminescent Assay, Human)
• Mitchell CM, Balkus J, Agnew KJ, Cohn S, Luque A, Lawler R, Coombs RW, Hitti JE Bacterial vaginosis, not HIV, is primarily responsible for increased vaginal concentrations of proinflammatory cytokines. AIDS Res. Hum. Retroviruses, 2008-05-01;24(5):667-71. 2008-05-01 [PMID: 18462081] (ELISA Development, Human)
• Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res. Hum. Retroviruses, 2008-03-01;24(3):453-62. 2008-03-01 [PMID: 18327985] (Luminex Development, Human)
• Pfleger C, Schloot N, ter Veld F Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J. Immunol. Methods, 2007-10-22;329(1):214-8. 2007-10-22 [PMID: 17964594] (Luminex Development, Human)
• Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P Ultrasensitive flow-based immunoassays using single-molecule counting. Clin. Chem., 2007-09-21;53(11):1990-5. 2007-09-21 [PMID: 17890441] (ELISA Development, Human)
• Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. AIDS, 2007;21(10):1263-72. 2007 [PMID: 17545702] (Luminex Development, Human)
• Emsley HC, Smith CJ, Gavin CM Clinical outcome following acute ischaemic stroke relates to both activation and autoregulatory inhibition of cytokine production. BMC Neurol, 2007-02-28;7(0):5. 2007-02-28 [PMID: 17328808] (ELISA Development, Human)
• Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood, 2007-02-08;109(10):4272-9. 2007-02-08 [PMID: 17289812] (Luminex Development, Human)
• Miranda LA, Braga F, Fischer RG, Sztajnbok FR, Figueredo CM, Gustafsson A Changes in periodontal and rheumatological conditions after 2 years in patients with juvenile idiopathic arthritis. J. Periodontol., 2006-10-01;77(10):1695-700. 2006-10-01 [PMID: 17032112] (ELISA Development, Human)
• Peng H, Erdmann N, Whitney N, Dou H, Gorantla S, Gendelman HE, Ghorpade A, Zheng J HIV-1-infected and/or immune activated macrophages regulate astrocyte SDF-1 production through IL-1beta. Glia, 2006-11-01;54(6):619-29. 2006-11-01 [PMID: 16944452] (ELISA Development, Human)
• Kiryushko D, Pavon L, Novitskaya V, Sandoval-Lopez G, Soroka V, Eugenia Hernandez M, Klingelhofer J, Loria F, Lukanidin E, Estrada I, Berezin V, Perez M, Bock E, Moreno J, Avila U, Leff P, Anton B, Heinze G Th2 cytokine response in Major Depressive Disorder patients before treatment. J. Neuroimmunol., 2006-02-02;172(1):156-65. 2006-02-02 [PMID: 16457895] (ELISA Development, Human)
• Chen DS, Soen Y, Stuge TB Marked differences in human melanoma antigen-specific T cell responsiveness after vaccination using a functional microarray. PLoS Med., 2005-09-20;2(10):e265. 2005-09-20 [PMID: 16162034] (Functional Assay, Human)
• Deiva K, Geeraerts T, Salim H, Leclerc P, Hery C, Hugel B, Freyssinet JM, Tardieu M Fractalkine reduces N-methyl-d-aspartate-induced calcium flux and apoptosis in human neurons through extracellular signal-regulated kinase activation. Eur. J. Neurosci., 2004-12-01;20(12):3222-32. 2004-12-01 [PMID: 15610155] (ICC, IHC-Fr, Human)
• Copeland S, Siddiqui J, Remick D Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. J. Immunol. Methods, 2004-01-01;284(1):99-106. 2004-01-01 [PMID: 14736420] (Array Development, Human)

Bioinformatics

• Gene Symbol: IL1B
• Uniprot:
  • NP_000567()