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IKK epsilon/IKBKE [p Thr501] Antibody

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 (1 Review)
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

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IKK epsilon/IKBKE [p Thr501] Antibody Summary

Immunogen
IKKe phospho peptide corresponding to a region of the human protein surrounding [p Thr501] conjugated to KLH.
Modification
p Thr501
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IKBKE
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • ELISA 1:5000-1:25000
Application Notes
Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. An 85 kDa band corresponding to human IKKe is detected. HeLa cells or TNF inducible KBM-5 cells can be used as a positive control.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-77829 in the following applications:
Publications
Read Publications using
NBP1-77829 in the following applications:

  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IKK epsilon/IKBKE [p Thr501] Antibody

  • EC 2.7.11
  • EC 2.7.11.10
  • I-kappa-B kinase epsilon
  • IKBKE
  • IKK epsilon
  • IKK-E
  • IKKEMGC125297
  • IKK-epsilon
  • IKKI
  • IKK-I
  • IKK-iMGC125295
  • Inducible I kappa-B kinase
  • inducible IkappaB kinase
  • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
  • inhibitor of nuclear factor kappa-B kinase subunit epsilon
  • KIAA0151IKK-related kinase epsilon
  • MGC125294

Background

Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibit NF-kB activity. IkB proteins are phosphorylated by an IkB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg. Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKe is required for the activation of NF-kB by mitogens and T cell receptors but not by TNFaor IL-1. LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IkBa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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(26 Publications)
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Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
HGK/MAP4K4
Antibody (4A5)
H00009448-M07
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(2 Publications)
Species: Hu, Bv, Pa
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
JNK1 Antibody
(228601) [Unconjugate
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Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
JNK1 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(2 Reviews)
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC
TANK Antibody
NB100-56144
Immunohistochemistry: TANK Antibody - Unpurified [NB100-56144] - TANK Antibody [NB100-56144] - Immunohistochemical analysis of TANK/I-TRAF1 expression in formalin-fixed, paraffin-embedded normal human colon using this antibody at 1:2000. A-A3, low and high magnification images. Hematoxylin-eosin counterstain.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
IKK epsilon/IKBKE
[p Thr501] Antibody
NBP1-77829
IKK epsilon/IKBKE [p Thr501] Antibody
Species: Hu
Applications: WB, ELISA

Publications for IKK epsilon/IKBKE Antibody (NBP1-77829)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Review for IKK epsilon/IKBKE Antibody (NBP1-77829) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-77829:
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Images Ratings Applications Species Date Details
Western Blot IKK epsilon/IKBKE NBP1-77829
Enlarge 		5
reviewed by:
Preeti Bharaj
 WB Human 12/21/2017
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Summary

ApplicationWestern Blot
Sample Tested293 whole cell lysate
SpeciesHuman
Lot30472

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3
0
2
0
1
0
Western Blot IKK epsilon/IKBKE NBP1-77829
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 Preeti Bharaj
12/21/2017
Application: WB
Species: Human
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Bioinformatics

Gene Symbol IKBKE
Entrez
Uniprot