IKK epsilon/IKBKE [p Thr501] Antibody - BSA Free

• Reactivity: Hu
• Applications: WB, Simple Western, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IKK epsilon/IKBKE [p Thr501] Antibody - BSA Free Summary

• Description: This antibody was affinity purified from monospecific antiserum by immunoaffinity purification against the phosphopeptide and cross adsorption against the non-phosphorylated form of the peptide followed by non-adsorption against a non-specific peptide backbone to further reduce non-specific reactivity
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: IKK epsilon/IKBKE phospho peptide corresponding to a region of the human protein surrounding pT501 conjugated to KLH. (Uniprot: Q14164)
• Modification: p Thr501
• Specificity: This phospho specific polyclonal antibody is specific for phosphorylated pT501 human IKK epsilon/IKBKE. Reactivity with non-phosphorylated IKK epsilon/IKBKE is minimal. Cross reactivity with pT501 phosphorylated IKK epsilon/IKBKE from mouse, rat or other species has not been determined.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IKBKE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:25000
  • Simple Western
  • Western Blot 1:500-1:3000
• Application Notes: This product is tested in ELISA, western blotting, and although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. An 85 kDa band corresponding to human IKKe is detected. HeLa cells or TNF inducible KBM-5 cells can be used as a positive control. Researchers should determine optimal titers for other applications.
  
  See Simple Western Antibody Database for Simple Western validation: tested in HeLa-/+EGF, Jurkat/TNF; antibody dilution of 1:50; separated by charge

Reactivity Notes

This phospho specific polyclonal antibody is specific for phosphorylated pT501 human IKK epsilon/IKBKE. Reactivity with non-phosphorylated IKK epsilon/IKBKE is minimal. Cross reactivity with pT501 phosphorylated IKK epsilon/IKBKE from mouse, rat or other species has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.1% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for IKK epsilon/IKBKE [p Thr501] Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.10
• I-kappa-B kinase epsilon
• IKBKE
• IKK epsilon
• IKK-E
• IKKEMGC125297
• IKK-epsilon
• IKKI
• IKK-I
• IKK-iMGC125295
• Inducible I kappa-B kinase
• inducible IkappaB kinase
• inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
• inhibitor of nuclear factor kappa-B kinase subunit epsilon
• KIAA0151IKK-related kinase epsilon
• MGC125294

Background

Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibit NF-kB activity. IkB proteins are phosphorylated by an IkB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg. Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKe is required for the activation of NF-kB by mitogens and T cell receptors but not by TNFaor IL-1. LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IkBa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IKK epsilon/IKBKE Antibody (NBP1-77829)(4)

Publications using NBP1-77829

• van Tol S, Atkins C, Bharaj P et al. VAMP8 Contributes to the TRIM6-Mediated Type I Interferon Antiviral Response during West Nile Virus Infection J. Virol. 2020-01-06 [PMID: 31694946] (WB)
• Bharaj P, Wang YE, Dawes BE et al. The Matrix Protein of Nipah Virus Targets the E3-Ubiquitin Ligase TRIM6 to Inhibit the IKKE Kinase-Mediated Type-I IFN Antiviral Response PLoS Pathog. 2016-09-01 [PMID: 27622505] (WB, Human)
• Rajsbaum R, Versteeg GA, Schmid S et al. Unanchored k48-Linked Polyubiquitin Synthesized by the E3-Ubiquitin Ligase TRIM6 Stimulates the Interferon-Ikk-epsilon kinase-Mediated Antiviral Response. Immunity. 2014-05-28 [PMID: 24882218] (WB, Human)
• Sweeney SE, Mo L, Firestein GS et al. Antiviral gene expression in rheumatoid arthritis: role of IKKepsilon and interferon regulatory factor 3. Arthritis Rheum 2007-03-01 [PMID: 17328045]

Review for IKK epsilon/IKBKE Antibody (NBP1-77829) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Preeti Bharaj WB Human 12/21/2017

Summary

• Application: Western Blot
• Sample Tested: 293 whole cell lysate
• Species: Human
• Lot: 30472

Bioinformatics

• Gene Symbol: IKBKE
• Entrez:
  • 9641(Human)
• Uniprot:
  • Q14164()
  • Q14164()
  • Q14164()
  • Q14164()
  • Q14164()