Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. An 85 kDa band corresponding to human IKKe is detected. HeLa cells or TNF inducible KBM-5 cells can be used as a positive control.
Read 1 Review rated 5 using NBP1-77829 in the following applications:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.1% Sodium Azide
Immunogen affinity purified
Alternate Names for IKK epsilon/IKBKE [p Thr501] Antibody
I-kappa-B kinase epsilon
Inducible I kappa-B kinase
inducible IkappaB kinase
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
inhibitor of nuclear factor kappa-B kinase subunit epsilon
KIAA0151IKK-related kinase epsilon
Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibit NF-kB activity. IkB proteins are phosphorylated by an IkB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg. Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKe is required for the activation of NF-kB by mitogens and T cell receptors but not by TNFaor IL-1. LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IkBa.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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