IGFBP-1 Antibody (176110)

• Reactivity: Mu
• Applications: WB
• Clone: 176110
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

IGFBP-1 Antibody (176110) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IGFBP-1
  Ala26-Asn272
  Accession # P47876
• Specificity: Detects mouse IGFBP-1 in direct ELISAs and Western blots. In Western blots, weak cross-reactivity with recombinant mouse (rm) IGFBP-5 is observed and no cross-reactivity with recombinant human (rh) IGFBP-1, rmIGFBP-2, rmIGFBP-3, rhIGFBP-4, rmIGFBP-6, or rhIGFBP-7 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: IGFBP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGFBP-1 Antibody (176110)

• alpha-pregnancy-associated endometrial globulin
• amniotic fluid binding protein
• binding protein-25
• binding protein-26
• binding protein-28
• growth hormone independent-binding protein
• hIGFBP-1
• IBP1
• IBP-1
• IGF-binding protein 1
• IGFBP1
• IGFBP-1
• IGF-BP25
• insulin-like growth factor binding protein 1
• insulin-like growth factor-binding protein 1
• Placental protein 12
• PP12AFBP

Background

The insulin-like growth factor binding protein (IGFBP) family consists of six structurally related proteins that bind IGF with high affinity (1, 2). These proteins share conserved cysteine-rich N- and C- terminal regions that participate in IGF binding. IGFBPs regulate the bioavailability of IGFs and modulate their biological activities, both positively and negatively. Some IGFBPs also have intrinsic bioactivity that is IGF-independent. Post-transitional modifications of the IGFBPs, including glycosylation, phosphorylation and proteolysis, influence IGF binding affinities and tissue localization, affecting both the IGF-dependent and independent functions (1, 2). Mouse IGFBP-1 cDNA encodes a 272 amino acid (aa) precursor protein with a putative 25 aa signal peptide (3). Mature mouse IGFBP-1 contains potential phosphorylation and proteolytic cleavage sites, an Arg-Gly-Asp (RGD) integrin receptor recognition sequence, but lacks potential N-linked glycosylation sites. IGFBP-1 binds equally well to IGF-I and IGF-II. Phosphorylation of human IGFBP-1, but not rat IGFBP-1, increases IGF affinity. Mouse IGFBP-1 shares 67% and 93% aa sequence identity with human and rat IGFBP-1, respectively. IGFBP-1 is expressed in liver, decidua, and kidneys and is the most abundant IGFBP in amniotic fluid. Hepatic production of IGFBP-1 is down-regulated by insulin and up-regulated by glucocorticoids. Circulating IGFBP-1 levels are elevated under various catabolic conditions including bacterial or viral infections, trauma and diabetes (4). IGFBP-1 has been shown to either potentiate or inhibit the activities of IGF in a variety of cells. IGFBP-1, through its RGD motif, also interacts with alpha 5 beta 1 intergrin to stimulate changes in cell adhesion and migration in the absence of IGFs (1, 2).

1. Firth, S.M. and R.C. Baxter (2002) Endocrine Reviews 23:824.
2. Rajaram, S. et al. (1997) Endocrine Reviews 18:801.
3. Schuller, A.G. et al. (1994) Mol. Cell. Endocrinol. 104:57.
4. Lang, C.H. et al. (2003) Endocrinology 144:3922.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGFBP-1 Antibody (MAB1240)(4)

Publications using MAB1240

• M Lee, SH Kim, JH Jhee, TY Kim, HY Choi, HJ Kim, HC Park Microparticles derived from human erythropoietin mRNA-transfected mesenchymal stem cells inhibit epithelial-to-mesenchymal transition and ameliorate renal interstitial fibrosis Stem Cell Res Ther, 2020-09-29;11(1):422. 2020-09-29 [PMID: 32993806] (Western Blot, Human)
• Parrella E, Maxim T, Maialetti F, Zhang L, Wan J, Wei M, Cohen P, Fontana L, Longo V Protein restriction cycles reduce IGF-1 and phosphorylated Tau, and improve behavioral performance in an Alzheimer's disease mouse model. Aging Cell, 2013-03-11;12(2):257-68. 2013-03-11 [PMID: 23362919] (ELISA Development (Capture), Mouse)
• Williams NG, Interlichia JP, Jackson MF Endocrine actions of myostatin: systemic regulation of the IGF and IGF binding protein axis. Endocrinology, 2010-12-08;152(1):172-80. 2010-12-08 [PMID: 21147879] (ELISA Development, Mouse)
• Yamada PM, Mehta HH, Hwang D Evidence of a role for insulin-like growth factor binding protein (IGFBP)-3 in metabolic regulation. Endocrinology, 2010-10-06;151(12):5741-50. 2010-10-06 [PMID: 20926583] (ELISA Development, Mouse)

Bioinformatics

• Gene Symbol: IGFBP1
• Uniprot:
  • P47876()