IGFBP-1 Antibodies

871-B1), Recombinant Mouse IGFBP-1 (Catalog # 1588-B1) and Recombinant Human IGFBP-5 (Catalog # 875-B5). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IGFBP-1 Monoclonal Antibody (Catalog # MAB675) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGFBP-1 at approximately 26 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox"> 871-B1), Recombinant Mouse IGFBP-1 (Catalog # 1588-B1) and Recombinant Human IGFBP-5 (Catalog # 875-B5). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IGFBP-1 Monoclonal Antibody (Catalog # MAB675) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGFBP-1 at approximately 26 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Human IGFBP-1 (Catalog # 871-B1), Recombinant Mouse IGFBP-1 (Catalog # 1588-B1) and Recombinant Human IGFBP-5 (Catalog # 875-B5). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IGFBP-1 Monoclonal Antibody (Catalog # MAB675) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGFBP-1 at approximately 26 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

871-B1) inhibits Recombinant Human IGF-I (Catalog # 291-G1) induced proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF-I (6 ng/mL) activity elicited by Recombinant Human IGFBP-1 (5 µg/mL) is neutralized (green line) by increasing concentrations of Human IGFBP-1 Monoclonal Antibody (Catalog # MAB675). The ND₅₀ is typically 10-40 µg/mL." class="big_lightbox"> 871-B1) inhibits Recombinant Human IGF-I (Catalog # 291-G1) induced proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF-I (6 ng/mL) activity elicited by Recombinant Human IGFBP-1 (5 µg/mL) is neutralized (green line) by increasing concentrations of Human IGFBP-1 Monoclonal Antibody (Catalog # MAB675). The ND₅₀ is typically 10-40 µg/mL." class="big_thumb" />

871-B1) was serially diluted and captured by Mouse Anti-Human IGFBP‑1 Monoclonal Antibody (Catalog # MAB675) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGFBP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF871) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 871-B1) was serially diluted and captured by Mouse Anti-Human IGFBP‑1 Monoclonal Antibody (Catalog # MAB675) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGFBP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF871) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing liver. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing liver. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="IGFBP-1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse IGFBP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1240) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing liver. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF019). A specific band was detected for IGFBP-1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF019). A specific band was detected for IGFBP-1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IGFBP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF871) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IGFBP-1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

871-B1) was serially diluted and captured by Mouse Anti-Human IGFBP‑1 Monoclonal Antibody (Catalog # MAB675) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGFBP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF871) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 871-B1) was serially diluted and captured by Mouse Anti-Human IGFBP‑1 Monoclonal Antibody (Catalog # MAB675) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGFBP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF871) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />