IGF-II R/IGF2R Antibody (372604) [Unconjugated]

IGF-II R/IGF2R was detected in immersion fixed A172 human glioblastoma cell line using Mouse Anti-Human IGF-II R/IGF2R Monoclonal Antibody (Catalog # MAB2447) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 372604
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IGF-II R/IGF2R Antibody (372604) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IGF-II R/IGF2R
  Ser1510-Phe2108
  Accession # P11717.2
• Specificity: Detects human IGF-II R/IGF2R in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IGF‑I R/IGF1R is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGF2R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGF-II R/IGF2R Antibody (372604) [Unconjugated]

• 300 kDa mannose 6-phosphate receptor
• cation-independent mannose-6 phosphate receptor
• cation-independent mannose-6-phosphate receptor
• CD222 antigen
• CD222
• CI Man-6-P receptor
• CIMPR
• CI-MPR
• IGF2R
• IGF-II R
• IGF-II receptor
• IGFIIR
• IGF-IIR
• insulin-like growth factor 2 receptorM6P/IGF2R
• Insulin-like growth factor II receptor
• M6P/IGF2 receptor
• M6PR
• M6P-R
• MPR 300
• MPR1
• MPRI
• MPRIM6PR

Background

The type 2 insulin-like growth factor receptor (also known as cation-independent mannose-6 phosphate receptor/CI-MPR) is a 300 kDa member of the P-type lectin family of molecules. P-type lectins generate functional eukaryotic lysosomes by binding and sorting lysosomal enzymes expressing phosphorylated mannose residues (M6P) (1-3). IGF-II R is a type I transmembrane glycoprotein that contains a 2,264 amino acid (aa) extracellular region, a 23 aa transmembrane segment and a 124 aa cytoplasmic tail (4, 5). The extracellular region consists of 15 contiguous “binding” repeats of about 150 aa each. The odd-numbered repeats interact with “ligands” while the even-numbered repeats likely generate a nondisulfide homodimer in the membrane (1). Repeat #11 binds IGF-II, while repeats 3 and 9 bind mannose-6 phosphate; repeat #13 contains a fibronectin type II motif and assists in IGF-II binding (1, 2). In the extracellular region of IGF-II R expressed by R&D Systems (600 aa’s), human IGF-II R is 85% aa identical to both mouse and bovine IGF-II R. This expressed region includes binding repeats #11, 12, and 13. In addition to IGF-II, CI-MPR/IGF-II R binds non-M6P containing ligands such as retinoic acid, urokinase-type plasminogen-activator receptor and plasminogen, plus M6P-containing molecules such as lysosomal enzymes, TGF-beta 1 precursor, proliferin, LIF, CD26, herpes simplex glycoprotein D and granzymes A and B (2, 6). IGF-II R regulates many diverse biological functions that range from intracellular trafficking to the internalization of extracellular factors and modulation of cellular responses. It delivers newly synthesized M6P-tagged lysosomal enzymes from the trans-golgi network to endosomes, and facilitates the clearance of extracellular lysosomal and matrix degrading enzymes by internalization into clathrin-coated vesicles and delivery into endosomes. With respect to IGF-II biology, it would appear that IGF-II R is principally a regulator of local IGF-II levels, targeting IGF-II for destruction in lysosomes (2). However, some evidence suggests the receptor will signal via G‑proteins, an effect that has yet to be conclusively shown (6).

1. Ghosh, P. et al. (2003) Nat. Rev. Mol. Cell. Biol. 4:202.
2. Dahms, N.M. and M.K. Hancock (2002) Biochim. Biophys. Acta. 1572:317.
3. Zaina, S. and J. Nilsson (2003) Curr. Opin. Lipidol. 14:483.
4. Morgan, D.O. et al. (1987) Nature 329:301.
5. Oshima, A. et al. (1988) J. Biol. Chem. 263:2553.
6. Hawkes, C. and S. Kar (2004) Brain Res. Rev. 44:117.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGF-II R/IGF2R Antibody (MAB2447)(1)

Publication using MAB2447

• P Bella, A Farini, S Banfi, D Parolini, N Tonna, M Meregalli, M Belicchi, S Erratico, P D'Ursi, F Bianco, M Legato, C Ruocco, C Sitzia, S Sangiorgi, C Villa, G D'Antona, L Milanesi, E Nisoli, P Mauri, Y Torrente Blockade of IGF2R improves muscle regeneration and ameliorates Duchenne muscular dystrophy EMBO Mol Med, 2019-12-02;0(0):e11019. 2019-12-02 [PMID: 31793167] (Cell Culture, Mouse)

Bioinformatics

• Gene Symbol: IGF2R
• Uniprot:
  • P11717.2()