IGF-I/IGF-1 Antibody - BSA Free

Immunohistochemistry-Paraffin: IGF-I/IGF-1 Antibody [NBP2-16929] - Human breast carcinoma. IGF1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 antibody (NBP2-16929) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 antibody (NBP2-16929) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 antibody (NBP2-16929) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

IGF1 antibody detects IGF1 protein at Golgi apparatus membrane by immunofluorescent analysis.Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: IGF1 protein stained by IGF1 antibody (NBP2-16929) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Gp, RM, Sh
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IGF-I/IGF-1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IGF-I/IGF-1 Antibody - BSA Free (NBP2-16929) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-IGF-I/IGF-1 Antibody: Cited in 10 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human IGF-I/IGF-1. The exact sequence is proprietary.
• Localization: Secreted
• Predicted Species: Rat (94%), Porcine (96%), Bovine (97%), Guinea Pig (100%), Rhesus Macaque (100%), Canine (98%), Sheep (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGF1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Armadillo (100%), Xenopus laevis (80%), Rabbit (82%), Chicken (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for IGF-I/IGF-1 Antibody - BSA Free

• IBP1
• IGF1
• IGF-1
• IGF1A
• IGFI
• IGF-I
• IGF-IA
• IGF-IB
• insulin-like growth factor 1 (somatomedin C)
• insulin-like growth factor 1
• insulin-like growth factor I
• insulin-like growth factor IA
• insulin-like growth factor IB
• Mechano growth factor
• MGF
• Somatomedin A
• Somatomedin C
• somatomedin-C

Background

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for IGF-I/IGF-1 Antibody (NBP2-16929)(10)

Publications using NBP2-16929

• Suzuki T, Scala F, Demetrius DL et al. IL-15 Prolongs Anagen, Stimulates Proliferation, and Suppresses Apoptosis in the Hair Matrix of Human Scalp Hair Follicles The Journal of investigative dermatology 2023-08-04 [PMID: 37543243]
• Shamardl HAMA, Ibrahim NA, Merzeban DH et al. Resveratrol and Dulaglutide ameliorate adiposity and liver dysfunction in rats with diet-induced metabolic syndrome: Role of SIRT-1 / adipokines / PPAR? and IGF-1 Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences 2023-03-29 [PMID: 36991247] (IHC, Rat)
• Ladraa S, Zerbib L, Bayard C et al. PIK3CA gain-of-function mutation in adipose tissue induces metabolic reprogramming with Warburg-like effect and severe endocrine disruption Science advances 2022-12-09 [PMID: 36490341]
• Jia ZQ, Zuo C, Yue WF Kurarinone alleviates hemin-induced neuroinflammation and microglia-mediated neurotoxicity by shifting microglial M1/M2 polarization via regulating the IGF1/PI3K/Akt signaling The Kaohsiung journal of medical sciences 2022-09-28 [PMID: 36169245] (WB, Human)
  
  Details:
  Dilution used in WB 1:1000
• Luo M, Zeng Q, Jiang K et al. Estrogen deficiency exacerbates learning and memory deficits associated with glucose metabolism disorder in APP/PS1 double transgenic female mice Genes & Diseases 2022-09-01 [PMID: 35873026]
• Yan X, Managlia E, Zhao YY et al. Macrophage-derived IGF-1 protects the neonatal intestine against necrotizing enterocolitis by promoting microvascular development Communications biology 2022-04-06 [PMID: 35388142] (WB, Mouse)
• MaihOfer J, Madry H, Rey-Rico A et al. Hydrogel-Guided, rAAV-Mediated IGF-I Overexpression Enables Long-Term Cartilage Repair and Protection against Perifocal Osteoarthritis in a Large-Animal Full-Thickness Chondral Defect Model at One Year In Vivo Advanced materials (Deerfield Beach, Fla.) 2021-03-18 [PMID: 33734514] (IF/IHC)
• Shymaa M. El-Komy, Ayman A. Saleh, Tamer M. Abdel-Hamid, Mohammed A. El-Magd Association of GHR Polymorphisms with Milk Production in Buffaloes Animals : an Open Access Journal from MDPI 2020-07-15 [PMID: 32679878]
• Song J, Hao L, Wei W et al. A SNP in the 3'UTR of porcine IGF-1 gene interacts with miR-new14 to affect IGF-1 expression, proliferation and apoptosis of PK-15 cell Domest Anim Endocrinol 2020-03-15 [PMID: 32171113]
• Lin CW Jan MS Kuo JH. Exploring MicroRNA Expression Profiles Related to the mTOR Signaling Pathway in Mouse Embryonic Fibroblast Cells Treated with Polyethylenimine. Mol Pharm. [PMID: 26158199]

Review for IGF-I/IGF-1 Antibody (NBP2-16929) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Andrew Cosgrove WB Mouse 12/16/2013

Summary

• Application: Western Blot
• Sample Tested: Mouse
• Species: Mouse
• Lot: 40807

Bioinformatics

• Gene Symbol: IGF1