IGF-I/IGF-1 Antibody

Western Blot: IGF-I/IGF-1 Antibody [NBP2-16929] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IGF1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: IGF-I/IGF-1 Antibody [NBP2-16929] - SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF1 protein stained by IGF1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: IGF-I/IGF-1 Antibody [NBP2-16929] - Human breast carcinoma. IGF1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Gp, RM, Sh
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

IGF-I/IGF-1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human IGF1. The exact sequence is proprietary.
• Localization: Secreted
• Predicted Species: Rat (94%), Porcine (96%), Bovine (97%), Guinea Pig (100%), Rhesus Macaque (100%), Canine (98%), Sheep (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (80%), Rabbit (82%), Chicken (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol (pH7).
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for IGF-I/IGF-1 Antibody

• IBP1
• IGF1
• IGF-1
• IGF1A
• IGFI
• IGF-I
• IGF-IA
• IGF-IB
• insulin-like growth factor 1 (somatomedin C)
• insulin-like growth factor 1
• insulin-like growth factor I
• insulin-like growth factor IA
• insulin-like growth factor IB
• Mechano growth factor
• MGF
• Somatomedin A
• Somatomedin C
• somatomedin-C

Background

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for IGF-I/IGF-1 Antibody (NBP2-16929)(5)

Publications using NBP2-16929

• Jia ZQ, Zuo C, Yue WF Kurarinone alleviates hemin-induced neuroinflammation and microglia-mediated neurotoxicity by shifting microglial M1/M2 polarization via regulating the IGF1/PI3K/Akt signaling The Kaohsiung journal of medical sciences 2022-09-28 [PMID: 36169245] (WB, Human)
  
  Details:
  Dilution used in WB 1:1000
• Yan X, Managlia E, Zhao YY et al. Macrophage-derived IGF-1 protects the neonatal intestine against necrotizing enterocolitis by promoting microvascular development Communications biology 2022-04-06 [PMID: 35388142] (WB, Mouse)
• MaihOfer J, Madry H, Rey-Rico A et al. Hydrogel-Guided, rAAV-Mediated IGF-I Overexpression Enables Long-Term Cartilage Repair and Protection against Perifocal Osteoarthritis in a Large-Animal Full-Thickness Chondral Defect Model at One Year In Vivo Advanced materials (Deerfield Beach, Fla.) 2021-03-18 [PMID: 33734514] (IHC)
• Song J, Hao L, Wei W et al. A SNP in the 3'UTR of porcine IGF-1 gene interacts with miR-new14 to affect IGF-1 expression, proliferation and apoptosis of PK-15 cell Domest Anim Endocrinol 2020-03-15 [PMID: 32171113]
• Lin CW Jan MS Kuo JH. Exploring MicroRNA Expression Profiles Related to the mTOR Signaling Pathway in Mouse Embryonic Fibroblast Cells Treated with Polyethylenimine. Mol Pharm. [PMID: 26158199]

Review for IGF-I/IGF-1 Antibody (NBP2-16929) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 12/16/2013

Summary

• Application: Western Blot
• Sample Tested: Mouse
• Species: Mouse
• Lot: 40807

Bioinformatics

• Gene Symbol: IGF1