291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-I/IGF-1 (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA). The ND₅₀ is typically 3-12 µg/mL." class="big_lightbox"> 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-I/IGF-1 (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA). The ND₅₀ is typically 3-12 µg/mL." alt="Recombinant Human IGF-I/IGF-1 (291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-I/IGF-1 (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA). The ND₅₀ is typically 3-12 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

291-G1) was serially diluted and captured by Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 291-G1) was serially diluted and captured by Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

791-MG) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-I/IGF-1 (15 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791). The ND₅₀ is typically 0.2-1.0 µg/mL." class="big_lightbox"> 791-MG) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-I/IGF-1 (15 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791). The ND₅₀ is typically 0.2-1.0 µg/mL." alt="Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-I/IGF-1 (15 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791). The ND₅₀ is typically 0.2-1.0 µg/mL." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing brain and muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing brain and muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

791-MG) was serially diluted and captured by Hamster Anti-Mouse IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB791) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 791-MG) was serially diluted and captured by Hamster Anti-Mouse IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB791) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

291-G1), Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG), Recombinant Rat IGF-I/IGF-1 (Catalog # 4326-RG), and Recombinant Human IGF-II (Catalog # 292-G2). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGF-I/IGF-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_lightbox"> 291-G1), Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG), Recombinant Rat IGF-I/IGF-1 (Catalog # 4326-RG), and Recombinant Human IGF-II (Catalog # 292-G2). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGF-I/IGF-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows 25 ng of Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1), Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG), Recombinant Rat IGF-I/IGF-1 (Catalog # 4326-RG), and Recombinant Human IGF-II (Catalog # 292-G2). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGF-I/IGF-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />

291-G1) was serially diluted and captured by Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 291-G1) was serially diluted and captured by Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

791-MG) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-I/IGF-1 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB791). The ND₅₀ is typically 0.3-1.5 µg/mL." class="big_lightbox"> 791-MG) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-I/IGF-1 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB791). The ND₅₀ is typically 0.3-1.5 µg/mL." alt="Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IGF-I/IGF-1 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB791). The ND₅₀ is typically 0.3-1.5 µg/mL." class="big_thumb" />

791-MG) was serially diluted and captured by Hamster Anti-Mouse IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB791) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 791-MG) was serially diluted and captured by Hamster Anti-Mouse IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB791) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF791) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in decidual cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in decidual cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="IGF-I/IGF-1 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human IGF-I/IGF-1 Recombinant Monoclonal Antibody (Catalog # MAB2911) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in decidual cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IGF-I/IGF-1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB2912). The ND₅₀ is typically 0.1-0.8 µg/mL." class="big_lightbox"> 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IGF-I/IGF-1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB2912). The ND₅₀ is typically 0.1-0.8 µg/mL." alt="Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IGF-I/IGF-1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB2912). The ND₅₀ is typically 0.1-0.8 µg/mL." class="big_thumb" />

NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="IGF-I/IGF-1 was detected in immersion fixed MDA-MB-123 human breast cancer cell line (left panel, positive stain) and PC-3 human prostate cancer cell line (right panel, negative stain) using Rat Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB2913) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />